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应用生态学报 ›› 1998, Vol. 9 ›› Issue (3): 257-260.

• 研究论文 • 上一篇    下一篇

胜红蓟化感作用研究 Ⅱ.主要化感物质的释放途径和活性

孔垂华, 徐涛, 胡飞   

  1. 华南农业大学热带亚热带生态研究所, 广州510642
  • 收稿日期:1997-11-10 修回日期:1998-03-28 出版日期:1998-05-25 发布日期:1998-05-25
  • 基金资助:

    国家自然科学基金(39670141);广东省自然科学青年基金(960427)资助项目

Allelopathy of Ageratum conyzoides Ⅱ. Releasing mode and activity of main allelochemicals

Kong Chuihua, Xu Tao, Hu Fei   

  1. Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642
  • Received:1997-11-10 Revised:1998-03-28 Online:1998-05-25 Published:1998-05-25

摘要: 研究了南方重要杂草胜红蓟(Ageratumconyzoides)挥发物质的化感作用。结果表明,胜红蓟鲜叶的挥发物质和挥发油对所有受试植物的幼苗生长有显着的抑制作用。用色谱法鉴定并分离了胜红蓟挥发油的主要成分早熟素Ⅰ和早熟素Ⅱ,两者分别占总量的30.93%和51.61%.胜红蓟水溶物中的早熟素Ⅰ和Ⅱ是从挥发物中而来,挥发是胜红蓟释放化感物质的主要途径。分离纯化的早熟素Ⅰ和Ⅱ的饱和水溶液对萝卜、番茄和黑麦草的幼苗生长均有显着的抑制作用,特别是早熟素Ⅱ具有强烈的化感作用,在25μg·ml-1低浓度下,仍能抑制植物幼苗的生长,但早熟素Ⅰ和Ⅱ的混合溶液对植物的抑制作用并无显着增强,两者之间不存在协同作用。

关键词: 胜红蓟, 化感作用, 释放途径, 早熟素, 高通量测序, nifH基因, 荧光定量PCR, 豆禾间作, 群落组成

Abstract: Studies on the allelopathy of volatile substances of Ageratum conyzoides, animportant weed in South China, show that the volatiles of its fresh leaves significantly inhibited the seedling growth of all test plants.By means of chromatography, precocene Ⅰ and Ⅱ were isolated and identified, accounting for 30.93% and 51.61% of the volatile oil, respectively. These two substances in aqueous extracts of the weed originated from its volatiles, indicating that volatilization was the main mode by which the weed released allelochemicals. The saturated aqueous solution of the isolated and purified precocene Ⅰ and Ⅱ also significantly inhibited the seedling growth of radish, tomato and ryegrass. Especially, precocene Ⅱ had so strong allelopathic activity that it could inhibit the seedling growth at low concentration of 25μg·ml-1 . The mixed solution of precocene Ⅰ and Ⅱ did not show a significantly increasing inhibition to plants, indicating that there was no synergistic effect between precocene Ⅰ and Ⅱ.

Key words: Ageratum conyzoides, Allelopathy, Releasing mode, Precocene, nifH gene, community composition, legume-oat intercropping, real-time PCR, high-throughput sequencing