长白山阔叶红松林优势树种叶际真菌群落结构

doi:10.13287/j.1001-9332.202209.035

应用生态学报 ›› 2022, Vol. 33 ›› Issue (9): 2405-2412.doi: 10.13287/j.1001-9332.202209.035

长白山阔叶红松林优势树种叶际真菌群落结构

王星^1,2, 杨腾³, 毛子昆¹, 蔺菲¹, 叶吉¹, 房帅¹, 戴冠华¹, 胡家瑞¹, 郝占庆⁴, 王绪高^1,5, 原作强^1,4*

¹中国科学院沈阳应用生态研究所中国科学院森林生态与管理重点实验室, 沈阳 110016;
²中国科学院大学, 北京 100049;
³中国科学院南京土壤研究所, 南京 210008;
⁴西北工业大学生态环境学院, 西安 710072;
⁵辽宁省陆地生态系统碳中和重点实验室, 沈阳 110016

收稿日期:2021-09-02 接受日期:2022-07-06 出版日期:2022-09-15 发布日期:2023-03-15
通讯作者: * E-mail: zqyuan@iae.ac.cn.
作者简介:王星, 男, 1996年生, 硕士研究生。主要从事森林生物多样性研究。E-mail: 18511178023@163.com
基金资助:
美丽中国生态文明建设科技工程专项(XDA 23080301)、国家自然科学基金项目(32171581,31691133027)和辽宁省自然科学基金计划(2021-MS-028)资助。

Community structure of phyllosphere fungi associated with dominant tree species in a broad-leaved Korean pine forest of Changbai Mountain, Northeast China

WANG Xing^1,2, YANG Teng³, MAO Zi-kun¹, LIN Fei¹, YE Ji¹, FANG Shuai¹, DAI Guan-hua¹, HU Jia-rui¹, HAO Zhan-qing⁴, WANG Xu-gao^1,5, YUAN Zuo-qiang^1,4*

¹Chinese Academy of Sciences Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
²University of Chinese Academy of Sciences, Beijing 100049, China;
³Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
⁴School of Ecology and Enviornment, Northwestern Polytechnical University, Xi’an 710072, China;
⁵Key Laboratory of Terrestrial Ecosystem Carbon Neutrality, Shenyang 110016, China

Received:2021-09-02 Accepted:2022-07-06 Online:2022-09-15 Published:2023-03-15

摘要/Abstract

摘要： 森林作为陆地生态系统的主体,其林冠承载了地球上大约40%的现存物种,叶际微生物作为冠层生物多样性的重要组成部分, 在维持植物健康、提高宿主抵御能力和参与全球生物地球化学循环等生态功能中发挥着重要作用,然而相对于根际微生物,目前关于冠层叶际微生物群落组成的研究还比较缺乏。本研究以吉林省长白山自然保护区内阔叶红松林为主要研究对象,使用高通量测序技术分析6种优势树种(红松、紫椴、蒙古栎、色木槭、水曲柳、春榆)的叶际真菌群落特征,并测定宿主的14种叶片功能性状,对比研究了不同优势树种叶际真菌群落组成与功能群异同,探讨真菌群落组成与叶片功能性状的关联性。结果表明:叶际真菌的优势门和纲分别为子囊菌门、担子菌门,座囊菌纲和外囊菌纲;不同树种林冠叶际真菌组成存在明显差异。LEfSe分析表明,除春榆和色木槭外,其余树种都有显著的差异指示种,如红松的圆孢多臂菌属和蒙古栎的柱隔孢属;对比FUNGuild数据库发现,叶际真菌主要功能群为病理寄生型;冗余分析和envfit分析结果表明,与植物养分获取和抵御病虫害相关的性状是影响林冠叶际真菌群落结构的主要因子。

关键词: 温带森林, 高通量测序, 群落结构, 功能预测, 叶片功能性状

Abstract: Forest is the main component of terrestrial ecosystems that harbors about 40% of the existing species on the earth. As a vital component of biodiversity, phyllosphere microbes in the canopy play a critical and unique role in maintaining plant health, improving host resistance, and influencing global biogeochemical cycle. However, the studies on the community structure of phyllosphere fungi in natural forests are scarce as compared to that on rhizosphere microbes. Consequently, we know litter about how phyllosphere fungi associates with leaf traits. In this study, we analyzed fungal community composition of canopy leaves of six dominant tree species (i.e., Pinus koraiensis, Tilia amurensis, Quercus mongolica, Acer mono, Fraxinus mandshurica, and Ulmus japonica), in a broad-leaved Korean pine forest of Changbai Mountain Nature Reserve in Jilin Province, using high-throughput sequencing. We compared the differences of phyllosphere fungal community structure and functional groups of different dominant tree species. Moreover, 14 key leaf functional traits of their host trees were measured to investigate the relationships between fungal community composition and leaf functional traits. We found that the dominant phyla and class of phyllosphere fungi were Ascomycota and Basidiomycota, and Dothideomycetes and Taphrinomycetes, respectively. Results of LEfSe analysis indicated that all the tree species except Ulmus japonica had significant biomarkers, such as the Eurotiomycetes of Pinus koraiensis and the Ascomycetes of Quercus mongolica. The main functional groups of phyllosphere fungi were pathotroph. The results of redundancy and envfit analysis showed that functional traits related to plant nutrient acquisition as well as resistance to diseases and pests were the main factors influencing the community structure of phyllosphere fungi.

Key words: broad-leaved Korean pine forest, ITS high-throughput sequencing, community structure, functional prediction, leaf functional traits

王星, 杨腾, 毛子昆, 蔺菲, 叶吉, 房帅, 戴冠华, 胡家瑞, 郝占庆, 王绪高, 原作强. 长白山阔叶红松林优势树种叶际真菌群落结构[J]. 应用生态学报, 2022, 33(9): 2405-2412.

WANG Xing, YANG Teng, MAO Zi-kun, LIN Fei, YE Ji, FANG Shuai, DAI Guan-hua, HU Jia-rui, HAO Zhan-qing, WANG Xu-gao, YUAN Zuo-qiang. Community structure of phyllosphere fungi associated with dominant tree species in a broad-leaved Korean pine forest of Changbai Mountain, Northeast China[J]. Chinese Journal of Applied Ecology, 2022, 33(9): 2405-2412.

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长白山阔叶红松林优势树种叶际真菌群落结构

Community structure of phyllosphere fungi associated with dominant tree species in a broad-leaved Korean pine forest of Changbai Mountain, Northeast China

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