盐度和CO2倍增环境下碱蓬幼苗呼吸酶活性的变化

应用生态学报 ›› 1998, Vol. 9 ›› Issue (4): 354-358.

盐度和CO₂倍增环境下碱蓬幼苗呼吸酶活性的变化

刘家尧¹, 衣艳君¹, 白克智², 梁峥²

1. 曲阜师范大学生物系, 曲阜273165;
2. 中国科学院植物研究所, 北京100093

收稿日期:1997-12-23 修回日期:1998-04-21 出版日期:1998-07-25 发布日期:1998-07-25
基金资助:
国家自然科学基金重大项目(39393000);国家攀登计划;山东省自然科学基金(Y94D1244)资助项目

Variation of respiratory enzyme activities of Suaeda salsa seedlings under salinity and doubled CO₂

Liu Jiayao¹, Yi Yanjun¹, Bai Kezhi², Liang Zheng²

1. Department of Biology, Qufu Normal University, Qufu 273165;
2. Institute of Botany, Academia Sinica, Beijing 100093

Received:1997-12-23 Revised:1998-04-21 Online:1998-07-25 Published:1998-07-25

摘要/Abstract

摘要： 研究了生长在正常大气CO₂和CO₂倍增环境中的盐生植物碱蓬(Suaedasalsa)幼苗呼吸酶活性对KCl和NaCl的反应。结果表明,在CO₂倍增(700μl·L^-1)和正常大气CO₂(350μl·L^-1)下,300mmol·L^-1KCl和NaCl均能抑制琥珀酸脱氢酶(SDH)和苹果酸脱氢酶(MDH)活性,而异柠檬酸脱氢酶(IDH)活性为NaCl抑制、KCl促进； NaCl和KCl明显抑制细胞色素氧化酶(CO)和光呼吸中乙醇酸氧化酶(GO)、羟基丙酮酸还原酶(HPR)活性；并指出在KCl胁迫下,CO₂使三羧酸循环(TCAC)的运行变慢,NaCl胁迫下使其加快,TCAC运行限速步骤与MDH无关,CO为盐对呼吸代谢影响的重要位点。另外,K⁺、Na⁺对蛋白表达的影响有差异,CO₂可使盐胁迫下的碱蓬幼苗蛋白表达降低。

关键词: 碱蓬, KCl, NaCl, CO₂倍增, 呼吸酶

Abstract: Studies on the response of respiratory enzyme activities of halophyte Suaeda salsa seedlings growing under normal (350μl·L^-1) and doubled (700μl·L^-1) concentrations of CO₂ to KCl and NaCl show that under normal and doubled concentrations of CO₂, the activities of succinic dehydrogenase(SDH), malic dehydrogenase (MDH), Cytocrome Coxidase (CO), glycolate oxidase (GO) and hydroxypyruvate reductase (HPR) were inhibited by 300mmol·L^-1 KCl and NaCl;the activity of isocitric dehydrogenase (IDH) was decreased by NaCl, but increased by KCl.The operation rate of tricarboxylic acid cycle (TCAC) was deceleratedby CO₂ under KCl stress, but accelerated under NaCl salinity.The steps of limiting speed by TCAC's operation was not related with MDH.COis the important site of salt affecting respiratory metabolism.Moreover, the influence of K⁺ on protein expression was different from that of Na⁺, and CO₂ could reduce the protein expression of Suaeda salsa seedlings under NaCl and KCl stresses.

Key words: Suaeda salsa, KCl, NaCl, Doubled CO₂, Respiratory enzyme

刘家尧, 衣艳君, 白克智, 梁峥. 盐度和CO₂倍增环境下碱蓬幼苗呼吸酶活性的变化[J]. 应用生态学报, 1998, 9(4): 354-358.

Liu Jiayao, Yi Yanjun, Bai Kezhi, Liang Zheng. Variation of respiratory enzyme activities of Suaeda salsa seedlings under salinity and doubled CO₂[J]. Chinese Journal of Applied Ecology, 1998, 9(4): 354-358.

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盐度和CO₂倍增环境下碱蓬幼苗呼吸酶活性的变化

Variation of respiratory enzyme activities of Suaeda salsa seedlings under salinity and doubled CO₂

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