二氧化硫对地衣中共生藻菌营养关系影响研究

应用生态学报 ›› 2002, Vol. ›› Issue (2): 151-155.

二氧化硫对地衣中共生藻菌营养关系影响研究

孔繁翔, 胡伟, 桑伟莲, 王连生

南京大学环境学院污染控制与资源化研究国家重点实验室, 南京 210093

收稿日期:2001-07-19 修回日期:2001-09-12 出版日期:2002-02-15
通讯作者: 孔繁翔,男,1957年11月生,博士,教授,博士生导师.E-mail:kongfx@jlonline.com或kongfx3@nju.edu.cn
基金资助:
国家自然科学基金(39570150);教育部骨干教师基金资助项目

Effects of sulphur dioxide on the relationship between symbionts in lichen

KONG Fanxiang, HU Wei, SANG Weilian, WANG Liansheng

School of the Environment, State Key Laboratory of Pollution Control and Resources Reuse, Nanjing University, Nanjing 210093

Received:2001-07-19 Revised:2001-09-12 Online:2002-02-15

摘要/Abstract

摘要： 为探讨地衣对大气污染特别敏感的原因,分离淡腹黄梅衣(Xanthoparmellia mexicana)的共生藻和共生真菌,并重新合成地衣.研究了地衣及纯化培养的藻和菌在SO₂污染短期胁迫下的生理反应.结果表明,叶绿素a比叶绿素b易受伤害;叶绿素对0.5mg·L^-1的熏气最敏感,水溶液暴露时叶绿素PQa值的下降与SO₂的吸收量有相关性.地衣中酸性磷酸酶活性主要由共生藻所决定.丙二醇含量在共生藻和菌以及地衣样品中无显著差异;还原型谷胱甘肽GSH含量在共生菌中明显高于共生藻,并与SO₂胁迫强度密切相关.可以作为地衣过氧化状态和受损的生物标志物.共生真菌承担了地衣主要的抗氧化任务,由于物质与能量的消耗,共生藻比共生菌更容易受到伤害.

关键词: 淡腹黄梅衣, SO₂, 营养关系, 抗氧化系统

Abstract: Xanthoparmellia mexicana was successfully isolating and culturing from its two symbionts and,preliminarily re-symbiosising its thalli in vitro performed from pure cultured photobiont and mycobiont.The solution exposure method was applied,confirming the toxic effect of sulfite solution with pH4.0.The biochemical responses of lichen thalli and cultured symbionts to the short time stress of SO₂ were studied.The chlorophyll PQa value of photobiont was correlated with its absorption of SO₂.Chlorophyll a was more sensitive than chlorophyll b;chlorophyll was damaged most severely by the exposure of 0.5mg稬^-1 of SO₂ fumigation,but was aggravated with the increase in the concentration of SO₂ by way of solution exposure.The activity of acid phosphatase in lichen was mainly dependent on the photobiont.Reduced glutathione(GSH) was activated by oxidative stress of SO₂.GSHwas confirmed to be highly correlated with SO₂ stress,and was proposed to be a significant and prospective biomarker of the status of lichen antioxidant system and the oxidative damage in lichen.The mycobiont was proposed to mainly resist the oxidative stress by SO₂,while the photobiont was damaged more easily with energy depletion.

Key words: Xanthoparmellia mexicana, SO₂, Antioxidant defense system

中图分类号:

X171.5

孔繁翔, 胡伟, 桑伟莲, 王连生. 二氧化硫对地衣中共生藻菌营养关系影响研究[J]. 应用生态学报, 2002, (2): 151-155.

KONG Fanxiang, HU Wei, SANG Weilian, WANG Liansheng . Effects of sulphur dioxide on the relationship between symbionts in lichen[J]. Chinese Journal of Applied Ecology, 2002, (2): 151-155.

[1]	周丹, 李海燕, 王秀军, 李庆卫. 外源褪黑素对盐胁迫下银杏幼苗渗透调节和抗氧化能力的影响 [J]. 应用生态学报, 2024, 35(2): 431-438.
[2]	赵雷, 靳海笛, 曹晓云, 邓文辉, 杜灵娟. 六个东方铁筷子品种对干旱胁迫的生理响应及抗旱性评价 [J]. 应用生态学报, 2023, 34(10): 2644-2654.
[3]	钱必长, 赵晨, 赵继浩, 赖华江, 李向东, 刘兆新. 不同花生棉花间作模式对花生生育后期生理特性及产量的影响 [J]. 应用生态学报, 2022, 33(9): 2422-2430.
[4]	徐晨潇, 张晓宇, 刘超越, 刘昆, 毕焕改, 艾希珍. 外源褪黑素与钙离子互作对高温胁迫下黄瓜幼苗过氧化伤害的缓解效应 [J]. 应用生态学报, 2022, 33(10): 2725-2735.
[5]	张小琴, 张媛铃, 李炳言, 冯雅楠, 李萍, 张东升, 王利伟, 郝兴宇. CO₂浓度升高对大豆干旱胁迫的缓解效应 [J]. 应用生态学报, 2021, 32(1): 182-190.
[6]	刘兆新,刘婷如,刘妍,杨坚群,甄晓宇,栗鑫鑫,杨东清,李向东. 小麦行距配置对套种花生生理特性和产量的影响 [J]. 应用生态学报, 2018, 29(6): 1851-1959.
[7]	陈展彦, 武海涛, 王云彪, 吕宪国. 基于稳定同位素的湿地食物源判定和食物网构建研究进展 [J]. 应用生态学报, 2017, 28(7): 2389-2398.
[8]	杨微, 王红艳, 于开源, 娄虹, 纪光思, 阮亚男. 高浓度镉、锌及其复合作用对烟草抗氧化系统的影响 [J]. 应用生态学报, 2017, 28(6): 1948-1954.
[9]	丛毓, 贺红士, 谷晓楠, 徐文茹, 刘凯, 宗盛伟, 杜海波. 高山林线形成机理研究进展 [J]. 应用生态学报, 2016, 27(9): 3035-3041.
[10]	施钦, 殷云龙, 王芝权, 范文才, 华建峰. 中山杉及其父母本幼苗对干旱胁迫和复水的响应 [J]. 应用生态学报, 2016, 27(11): 3435-3443.
[11]	吴秀,陆晓民**. 亚适宜温光盐环境下油菜素内酯对黄瓜幼苗抗氧化系统及光合作用的影响 [J]. 应用生态学报, 2015, 26(9): 2751-2757.
[12]	江洪强1,邢兴华1,周琴1,江海东** . 外源α-萘乙酸对花期长期干旱大豆叶片抗氧化系统的影响 [J]. 应用生态学报, 2015, 26(6): 1718-1726.
[13]	陈红星,吴星,毕然,李莉霞,高蜜,李丹,谢凌天**. 水环境中Cr(Ⅵ)对鱼类毒性机理研究进展 [J]. 应用生态学报, 2015, 26(10): 3226-3234.
[14]	鲁艳**,雷加强,曾凡江,徐立帅,刘国军,彭守兰,黄彩变. NaCl胁迫对大果白刺幼苗生长和抗逆生理特性的影响 [J]. 应用生态学报, 2014, 25(3): 711-717.
[15]	庄明浩1,2,陈双林1,李迎春1,郭子武1,杨清平1. CO₂浓度升高对三种地被类观赏竹生理特性的影响 [J]. 应用生态学报, 2013, 24(9): 2408-2414.