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应用生态学报 ›› 2004, Vol. ›› Issue (8): 1344-1348.

• 研究论文 • 上一篇    下一篇

玉米根系内生细菌种群及动态分析

高增贵1, 庄敬华1, 陈捷1,2, 刘限1, 唐树戈1   

  1. 1. 沈阳农业大学植物保护学院农业部北方农作物病害免疫重点开放实验室, 沈阳 110161;
    2. 上海交通大学农业与生物学院, 上海 201101
  • 收稿日期:2002-10-12 修回日期:2003-02-28
  • 通讯作者: 陈捷
  • 基金资助:
    国家“十五”科学技术攻关资助项目(2001BA5090405)

Population of entophytic bacteria in maize roots and its dynamic analysis

GAO Zenggui1, ZHUANG Jinghua1, CHEN Jie1,2, LIU Xian1, TANG Shuge 1   

  1. 1. Key Laboratory of North Crop Immunology China's Ministry of Agriculture, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, China;
    2. Agriculture and Biology College, Shanghai Jiaotong University, Shanghai 201101, China
  • Received:2002-10-12 Revised:2003-02-28

摘要: 2000~2002年,先后对辽宁省14个玉米主栽品种进行了根系内主要细菌种群分析.结果表明,玉米内生细菌的主要种群为芽孢杆菌属(Bucillus spp.),此外还包括肠杆菌属、沙雷氏杆菌属、假单胞菌属、黄单胞菌属和棍状杆菌属.其中Bacillus分布最广,已鉴定出8个种,包括枯草芽孢杆菌、巨大芽孢杆菌、蜡状芽孢杆菌、地衣芽孢杆菌、炭疽芽孢杆菌、蕈状芽孢杆菌、短小芽孢杆菌、环状芽孢杆菌.Bacillus spp.总量占根系内生细菌总量比苗期和成株期分别为75.5%和76.6%.内生细菌在不同玉米品种和不同生育期之间存在程度不同的差异.研究发现,品种的遗传背景与其内生细菌的种类和数量显著相关.

关键词: 玉米, 内生细菌, 种群, 动态分析, 遗传背景

Abstract: In 2001~2002,14 maize cultivars in Liaoning Province were used for the analysis of their entophytic bacteria population.The entophytic bacteria strains with a higher frequency in maize roots were Bacillus spp.,Enterobacter spp.,Serratia spp.,Pseudomonas spp.,Xanthomonas spp.,Clavibacter spp.,Bacillus spp.,Enterobacter spp.and Serratia spp.Comparatively,Bacillus spp.was the most prevalent entophytic bacterium,including 8 species,B.subtilis,B.megaterium,B.cereus,B.licheniformis,B.anthracis, B.mycoides,B.pumilus and B.circulans,and with an average isolation frequency of 75.5% at seedling stage and 77.6% at adult stage.There existed significant differences in the population and dynamics of endophytic bacteria among maize cultivars and growth periods,and a significant correlation was found between maize genetic background and entophytic bacteria population.

Key words: Maize, Entophytic bacteria, Population, Dynamic analysis, Genetic background

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