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供氮水平对菠菜营养品质和体内抗氧化酶活性的影响

张英鹏;林咸永;章永松   

  1. 浙江大学环境与资源学院,杭州 310029

  • 收稿日期:2004-07-02 修回日期:2004-11-22 出版日期:2005-03-18

Effects of nitrogen supply on nutritional quality and antioxidative enzyme activities of spinach

ZHANG Yingpeng,LIN Xianyong,ZHANG Yongsong   

  1. College of Environment and Resource,Zhejiang University,Hangzhou 310029, China
  • Received:2004-07-02 Revised:2004-11-22 Online:2005-03-18

摘要: 通过水培实验,研究了供氮水平对菠菜营养品质和抗氧化酶活性的影响.结果表明,供氮水平由4 mmol·L-1增加到8 mmol·L-1,菠菜产量显著增加,叶片中的维生素C(Vc)含量随着供氮浓度由4 mmol·L-1提高到8 mmol·L-1,再提高供氮水平,Vc含量则明显下降.叶片硝酸盐含量随着氮浓度的提高而增加.供氮浓度从4 mmol·L-1增加到8 mmol·L-1,叶片可溶态草酸含量略有下降,再提高供氮水平则明显上升,而草酸总量随供氮水平提高,先显著升高然后略有降低.SOD和POD酶的活性随供氮水平由4 mmol·L-1提高到8 mmol·L-1而增加,再提高供氮水平,酶活性显著下降;CAT活性随供氮水平的增加而降低,叶片MDA含量先降低后显著升高,而游离脯氨酸含量随氮水平的升高而增加.可见供氮水平为8 mmol·L-1时,菠菜叶片具有较高的生物量、Vc含量和抗氧化酶活性,较低的硝酸盐和草酸含量以及较低的MDA和游离脯氨酸含量,表明供氮浓度8 mmol·L-1有利于提高菠菜的产量、营养品质和抗逆能力,是菠菜生长较适宜的供氮水平.

关键词: 城乡交错带, 景观格局, 南京市

Abstract: A hydroponic experiment was carried out to study the effects of nitrogen (N) supply on the nutritional quality and antioxidative enzyme activities of spinach.The results showed that when the N supply increased from 4 to 8 mmol·L-1,the biomass of spinach and its leaf vitamin C (Vc) content increased significantly,but a further increase of the N supply induced an obvious decrease of Vc content.An increasing N supply increased the nitrate content in leaves.The soluble oxalate content in leaves decreased slightly with the N supply from 4 to 8 mmol·L-1 N,but increased with a further increase of supplied N.Total oxalate content increased firstly,and then decreased with increasing N supply.SOD and POD activities increased with the N level up to 8 mmol·L-1 N,but drastically decreased with increasing N levels.CAT activity decreased when N level elevated.MDA content decreased firstly and then increased with increasing N levels,while free proline content decreased with increasing N supply.All of these indicated that 8 mmol·L-1 N was an appropriate N supply level for spinach to improve its biomass, nutritional quality,and resistance.

Key words: Urban-rural ecotone, Landscape pattern, Nanjing city