豆链格孢菌对白车轴草生理代谢及活性氧清除系统酶的影响

应用生态学报 ›› 2005, Vol. 16 ›› Issue (3): 529-532.

豆链格孢菌对白车轴草生理代谢及活性氧清除系统酶的影响

李征, 刘登义, 丁佳红, 王育鹏

安徽师范大学生物多样性研究中心, 重点生物资源保护与利用研究安徽省重点实验室, 芜湖, 241000

收稿日期:2004-06-29 修回日期:2004-10-18 出版日期:2005-03-15 发布日期:2005-03-15
通讯作者: 刘登义 E-mail:ldy@mail.ahnu.edu.cn
基金资助:
国家自然科学基金项目(30470270)、重要生物资源保护与利用研究安徽省重点实验室基金项目、安徽省自然科学基金项目(03043501)和教育部科学技术重点研究基金资助项目.

Effects of Alternaria azukiae on physiological metabolism and active oxygen eliminating enzyme activities of Trifolium repens

LI Zheng, LIU Dengyi, DING Jiahong, WANG Yupeng

Provincial Key Laboratory of Conservation and Exploitation of Biological Resources, Biodiversity Research Center, Anhui Normal University, Wuhu 241000, China

Received:2004-06-29 Revised:2004-10-18 Online:2005-03-15 Published:2005-03-15

摘要/Abstract

摘要： 通过盆栽实验,研究了接种豆链格孢菌对白车轴草叶组织细胞膜透性、色素含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响.结果表明,豆链格孢菌使白车轴草叶组织细胞膜透性上升,电导率显著升高,膜脂过氧化加强,MDA水平上升,色素含量下降.电导率和MDA含量均与感病时间呈显著正相关,而色素含量与感病时间呈显著负相关.豆链格孢菌刺激其寄主白车轴草叶片膜脂过氧化作用发生,积累大量的活性氧,使其超出防御酶的清除能力,致使活性氧清除系统遭到破坏,保护酶系统失衡,其中SOD和CAT活性显著下降,而POD活性明显上升.接种12d后,SOD和CAT分别降低了55.2%和37.8%,而POD比对照升高约1.6倍.

关键词: 豆链格孢菌, 白车轴草, 生理代谢, 酶活性

Abstract: A pot experiment was installed to study the effects of Alternaria azukiae inoculation on the cell membrane permeability,pigment and MDA contents,and activities of SOD,POD and CAT of Trifolium repens.The results showed that A.azukiae infection made T.repens leaf tissue increase its cell membrane permeability,electric conductivity,over oxidation and MDA content,but decrease its pigment content.The electric conductivity and MDA content were positively related with infection time,whereas the pigment content was negatively related with it.Due to the stimulation by A.azukiae,the over oxidation of cell membrane was intensified,which resulted in a large amount of active oxygen accumulated and beyond the defense ability,the active oxygen metabolism system was destroyed,and the balance of protective enzyme system was broken.After 12 days of A.azukiae inoculation,the activities of SOD and CAT decreased by 55.2% and 37.8%,respectively,while the POD activity increased by 1.6 times.

Key words: Alternaria azukiae, Trifolium repens, Physiological metabolism, Enzyme activity

中图分类号:

S₄32

李征, 刘登义, 丁佳红, 王育鹏. 豆链格孢菌对白车轴草生理代谢及活性氧清除系统酶的影响[J]. 应用生态学报, 2005, 16(3): 529-532.

LI Zheng, LIU Dengyi, DING Jiahong, WANG Yupeng . Effects of Alternaria azukiae on physiological metabolism and active oxygen eliminating enzyme activities of Trifolium repens[J]. Chinese Journal of Applied Ecology, 2005, 16(3): 529-532.

参考文献

[1] Alscher RG, Donahue JL, Cramer CL. 1997. Reactive oxygen species and antioxidants:Relationships in green cells. Physiol Plant, 100:224～233
[2] Chen J-X(陈建勋),Wang X-F(王晓峰).2002.Plant Physiology Experimentation. Guangzhou:South China Science and Engineering University Press. (in Chinese)
[3] Chis B, et al. 1992. Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Biol, 42(1):83～116
[4] Dong J-G(董金皋),Fan M-Z(樊慕贞),Han J-M(韩建民),et al.1999. The effect of AB-Toxin from Alternaria brassicae on membrane permeability and the activities of SOD and POD in cell of Chinese cabbageleaves.Acta Phytopathol Sin(植物病理学报),29(2):138～141 (in Chinese)
[5] Fridovich I. 1975. The biology of oxygen radical. Science, 201:875～880
[6] Hu D-X(胡迪先),Feng C-B(封朝壁),Zhu B-C(朱邦长).1994.The study on development of white clover.Acta Pratac Sin(草业学报),3(2):44～50(in Chinese)
[7] Hu F(胡飞),Kong C-H(孔垂华),Xu X-H(徐效华),et al.2002. Inhibitory effect of flavones from Ageratum conyzoides on the major pathogensin citrus orchard.Chin J Appl Ecol(应用生态学报),13(9):1166～1168(in Chinese)
[8] Jin Z(金珠),Kuang J(匡晶),Hong F-S(洪法水),et al.1999.Studies on the physiological basis of resistance of Portulaca oleracea to Peronospcra parasitica. J Huaibei Coal Ind Teach Coll(淮北煤炭师范学院学报),20(3):55～59(in Chinese)
[9] Ju H-Y(鞠会艳),Han L-M(韩丽梅),Wang S-Q(王树起),et al.2002. Allelopathic effect of root exudates on pathogenic fungi of root rotin continuous cropping soybean.Chin J Appl Ecol(应用生态学报),13(6):723 727(in Chinese)
[10] Kan G-F(阚光锋),Zhang G-M(张广民),Fang B-H(房保海),et al. 2002. The effect of Pseudomonas syringae pv. tabaci on the defendant enzymesin tobacco cell.J Shandong Agric Univ(山东农业大学学报),33(1):28～31(in Chinese)
[11] Kong C-H(孔垂华),Xu T(徐涛),Hu F(胡飞).1998.Allelopathy of Ageratum conyzoides Ⅱ. Releasing mode and activity ofmain allelochemicals.Chin J Appl Ecol(应用生态学报),9(3):257～260(in Chinese)
[12] Li J(李靖),Li R-Q(利容千),Yuan W-J(袁文静).1991.Changes of enzyme activity of the cucumber leaves after the infection of Peronospora parasitica.Acta Phytopathol Sin(植物病理学报),21(4):277～282(in Chinese)
[13] Liu D-Y(刘登义),Wang Y-B(王友保),Zhang X-X(张徐祥),et al. 2002. Effect of sewage irrigation on wheat growth and its activateoxygen metablism.Chin J Appl Ecol(应用生态学报),13(10):1319～1322(in Chinese)
[14] Liu D-Y(刘登义),Wang Y-B(王友保),Lars E.2003.Relationship between resistance and growth of Trifolium repens plants and their disease history.Chin J Appl Ecol(应用生态学报),14(1):35～42 (in Chinese)
[15] Panla KP, Thompson JE. 1984. Evidence for the accumulation of peroxidized lipids in membranes of senescing cotyledons. Plant Physiol, 75:1152～1157
[16] Proinoke XH. 1981. The Analysis Methods of Biochemistry of Plants. Beijing:Science Press. 197～209
[17] Scandalios JG. 1993. Oxygen stress and superoxide dismutase.Plant Physiol, 101(1):7～12
[18] Shandong Agricultural College(山东农学院), eds. 1980. Plant Physiology Experimentation. Jinan:Shandong Science and Technology Press. 109～112(in Chinese)
[19] WangA-G(王爱国).1986.The discussion of MDA be as the index of superoxide of plants.Plant Physiol Comm(植物生理学通讯),6(2):55～58(in Chinese)
[20] Wan Z-X(万佐玺),Zhu J-J(朱晶晶),Qiang S(强胜).2001.The phathogenic mechanism of toxin of Alternaria alternate(Fr.)Keissler to Eupatorium adenophorum Spreng. J Plant Resour Environ(植物资源与环境学报),10(3);47～50(in Chinese)
[21] Zhang J-L(张教林),Cao K-F(曹坤芳).2002.The effect ofirridiance on photosynthetic capacity, heat dissipation, and antioxidants of two tropical rain forest tree species. Acta Phytoecol Sin, 26(6):639～646 (in Chinese)
[22] Zhu G-L(朱广廉),Zhong W-H(钟文海),Zhang A-Q(张爱琴).1990. Plant Physiology Experimentation. Beijing:Peking University Press. 51～53,126～130(in Chinese)
[23] Zhu Z-J(朱祝军),Shou S-Y(寿森炎),Joska G,et al.1998.Effect of light intensity and nitrogen form on the activities of antioxidative enzymesin French bean.J Zhejiang Agric Univ(浙江农业大学学报),24(1):51～56(in Chinese)