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短期高温胁迫对高产小麦品系灌浆后期旗叶光系统Ⅱ功能的影响

陈锋1;田纪春2;孟庆伟1,3;赵世杰1,3   

  1. 1山东农业大学生命科学学院, 泰安 271018;2山东农业大学农学院, 泰安 271018;3山东省作物生物学重点实验室, 泰安 271018
  • 收稿日期:2005-10-31 修回日期:2006-07-31 出版日期:2006-10-18 发布日期:2006-10-18

Effects of short-term high temperature stress on flag leaf photosystem Ⅱ functions of high-yielding wheat at late grain-filling stage

CHEN Feng1; TIAN Jichun2; MENG Qingwei1,3; ZHAO Shijie1,3   

  1. 1College of Life Science, Shandong Agricultural University, Tai’an 271018, China;2College of Agronomy, Shandong Agricultural University, Tai’an 271018, China;3Key Laboratory of Crop Biology of Shandong Province, Tai’an 271018, China
  • Received:2005-10-31 Revised:2006-07-31 Online:2006-10-18 Published:2006-10-18

摘要: 以叶绿素快相荧光动力学曲线(OJIP)为探针,探讨了高温胁迫对高产小麦品系01-35灌浆后期光系统Ⅱ(PSⅡ)功能的影响.结果表明,在37 ℃~43 ℃范围内,随温度升高QA还原程度和还原速率增大,至43 ℃时分别比室温下增加了23.89%和24.09%,表明QA→QB的电子传递受到抑制;43 ℃时PSⅡ的电子受体库降至室温下的47.4%,表明高温胁迫伤害了PSⅡ受体库;而PSⅡ供体侧未受到影响.当温度达到46 ℃时,QA还原程度和还原速率分别比室温下增加了13.95%和20.48%,但比43 ℃时显著下降,而PSⅡ电子受体库与43 ℃时相比无显著变化,表明46 ℃时PSⅡ供体侧受到伤害.与对照品种鲁麦14相比,高温胁迫下高产小麦的捕光色素复合体仍能捕获较多的光能,而且将捕获的光能更多的用于电子传递,表明高产小麦的捕光色素复合体及电子传递体耐受高温的能力较强,能够维持较高的电子传递能力.

关键词: 兰花, 菌根菌, 分泌物, 抽提物

Abstract: By using fast OJIP chlorophyll a (Chl a) fluorescence transients, this paper studied the effects of short-term high temperature stress on the flag leaf photosystem Ⅱ (PSⅡ) functions of high-yielding winter wheat cultivar 01-35 at its late grain filling stage. The results showed that with the temperature raised from 37 ℃ to 43 ℃, the extent and velocity of QA reduction enhanced gradually, being 23.89% and 24.09% higher at 43 ℃ than at room temperature, respectively, which suggested that the electron transport from QA to QB was inhibited. The electron acceptor pool of PSⅡ including QA, QB and PQ at 43 ℃ decreased to 47.4% of that at room temperature, indicating its being damaged. The electron donor side of PSⅡ was not damaged from 37 ℃ to 43 ℃. When temperature raised to 46 ℃, the extent and velocity of QA reduction were 13.95% and 20.48% higher than those at room temperature, respectively, but decreased obviously, compared with those at 43 ℃. In contrast, the electron acceptor pool of PSⅡ at 46 ℃ had no obvious change, compared with that at 43 ℃, indicating that the electron donor side of PSⅡat 46 ℃ was damaged. Comparing with cultivar Lumai 14, the light harvesting complex (LHCⅡ) of high-yielding wheat cultivar 01-35 could harvest more sun energy, and distributed more absorbed energy into electron transport, suggesting that the LHCⅡ of cultivar 01-35 could endure a certain higher temperature, and maintain higher electron transport capacity.

Key words: Orchid, Mycorrhiza, Secretion, Extraction