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应用生态学报 ›› 2021, Vol. 32 ›› Issue (1): 299-308.doi: 10.13287/j.1001-9332.202101.036

• 研究论文 • 上一篇    下一篇

侧孢芽孢杆菌Bl13对番茄早疫病防治效果及机制

孙一凡, 刘喆, 李海洋, 郑泽浩, 纪成隆, 郭俏, 来航线*   

  1. 西北农林科技大学资源环境学院, 陕西杨凌 712100
  • 收稿日期:2020-05-07 接受日期:2020-10-19 出版日期:2021-01-15 发布日期:2021-07-15
  • 通讯作者: * E-mail: laihangxian@163.com
  • 作者简介:孙一凡, 男, 1994年生, 硕士研究生。主要从事微生物资源利用与植物保护研究。E-mail: 401862854@qq.com
  • 基金资助:
    陕西省重点研发计划项目(2019ZDLNY01-07-01)和国家自然科学基金项目(31600407)

Biocontrol effect and mechanism of Bacillus laterosporus Bl13 against early blight disease of tomato

SUN Yi-fan, LIU Zhe, LI Hai-yang, ZHENG Ze-hao, JI Cheng-long, GUO Qiao, LAI Hang-xian*   

  1. College of Natural Resources and Environment, Northwest A&F university, Yangling 712100, Shaanxi, China
  • Received:2020-05-07 Accepted:2020-10-19 Online:2021-01-15 Published:2021-07-15
  • Contact: * E-mail: laihangxian@163.com
  • Supported by:
    Shaanxi Key R&D Program Project (2019ZDLNY01-07-01) and the National Natural Science Foundation of China (31600407).

摘要: 以对番茄早疫病原菌有良好拮抗效果的侧孢芽孢杆菌Bl13为研究对象,采用盆栽试验,通过测定番茄株高、茎粗、番茄早疫病病情指数、叶片内防御酶活性以及根区土壤微生物多样性、微生物群落结构组成等指标,探究侧孢芽孢杆菌Bl13防治番茄早疫病的效果及机制。结果表明: 接种Bl13可显著降低番茄早疫病的病情指数,提高叶片内多酚氧化酶(PPO)、过氧化物酶(POD)等防御酶活性,降低病害对植物地上部分及根系生长发育的影响。同时,改善番茄根区土壤微生物群落结构,使芽孢杆菌属、假单胞菌属等常见有益菌属相对丰度显著提高,油壶菌属、血赤壳属相对丰度显著降低。侧孢芽孢杆菌Bl13可通过提高番茄叶片内防御酶活性并增加根区中有益微生物的数量来增强植物对番茄早疫病的抗性,从而实现对番茄早疫病的防治。

关键词: 侧孢芽孢杆菌, 番茄, 早疫病, 防御酶, 微生物群落结构

Abstract: Bacillus laterosporus Bl13 has good antagonistic effect on the pathogen of tomato early blight (TEB) disease. A pot experiment was conducted to investigate the effect and mechanism of B. laterosporus Bl13 against TEB disease by measuring biological traits such as plant height, stem diameter, disease index of TEB, activity of plant defense enzyme in leaves, and microbial diversity and community composition in root area. The results showed that B. laterosporus Bl13 could significantly reduce the disease index of TEB disease incidence, increase the activity of defensive enzymes including polyphenol oxidase (PPO) and peroxidase (POD) in leaves, and reduce the effects of the disease on the aboveground and root growth of tomato. Meanwhile, B. laterosporus Bl13 improved soil microbial community structure in the root zone, by significantly increasing the relative abundance of plant growth-promoting bacteria (Bacillus and Pseudomonas) and decreasing that of Olpidium and Haematonectria. The results showed that B. laterosporus Bl13 could enhance plant resistance against TEB disease through improving the activity of defensive enzymes in tomato leaves and increasing the number of beneficial microbes in the root zone.

Key words: Bacillus laterosporus, tomato, early blight, defensive enzyme, microbial community structure