铅胁迫对黄瓜幼苗抗氧化酶活性及同工酶的影响

应用生态学报

铅胁迫对黄瓜幼苗抗氧化酶活性及同工酶的影响

刘素纯¹;萧浪涛¹;廖柏寒¹;鲁旭东^1,2;匡逢春¹;赵文魁¹;童建华¹

¹湖南农业大学湖南省植物激素与生长发育重点实验室，长沙 410128；²孝感学院生命科学院，孝感 432000

收稿日期:2005-08-11 修回日期:2005-10-14 出版日期:2006-02-18 发布日期:2006-02-18

Effects of lead stress on anti-oxidative enzyme activities and isoenzymes in cucumber seedlings

LIU Suchun¹;XIAO Langtao¹;LIAO Bohan¹;LU Xudong^1,2;KUANG Fengchun¹;ZHAO Wenkui¹;TONG Jianhua¹

¹Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Hunan Agricultural University,Changsha 410128,China；²College of Life Sciences,Xiaogan University,Xiaogan 432000,China

Received:2005-08-11 Revised:2005-10-14 Online:2006-02-18 Published:2006-02-18

摘要/Abstract

摘要： 采用水培法和聚丙烯酰胺凝胶电泳法，研究铅胁迫对黄瓜幼苗过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及同工酶的影响.结果表明,铅胁迫下黄瓜幼苗地上部POD活性除第5天外均随铅浓度的增加而逐渐降低，POD同工酶谱带和表达量减少.在0～500 mg·L^-1铅浓度范围内，SOD活性随铅浓度的增加而增加，第7天达到最大值后急剧下降，低于同期对照值，900 mg·L^-1铅处理SOD活性随时间的延长逐步降低，SOD酶谱带和表达量与铅浓度呈负相关.CAT酶谱带无明显变化，而表达量存在差异.

关键词: 分子标记, 植物种群, AFLP, RAPD RFLP SSR

Abstract: With hydroponics and polyacrylamide gel electrophoresis (PAGE),this paper studied the effects of lead stress on the activities of peroxidase (POD),superoxide dismutase (SOD) and catalase (CAT),and their isoenzymic band numbers in cucumber seedlings.The results showed that the isoenzymic bands and expression quantity of POD decreased gradually with increasing lead concentration.The SOD activity increased with increasing lead concentration within the range of 0～500 mg Pb·L^-1,reached the maximum at 7th day,and then decreased rapidly,being lower than the control of the same period.Under the concentration of 900 mg Pb·L^-1,the SOD activity decreased with time.The isoenzymic bands and expression quantity of SOD were negatively related to lead concentration.No significant change was observed in the isoenzymic bands of CAT,but a significant difference was found in the expression quantity of CAT isoenzymes.

Key words: Molecular markers, Plant population, AFLP, RAPD, RFLP, SSR

刘素纯¹;萧浪涛¹;廖柏寒¹;鲁旭东^1,2;匡逢春¹;赵文魁¹;童建华¹. 铅胁迫对黄瓜幼苗抗氧化酶活性及同工酶的影响[J]. 应用生态学报.

LIU Suchun¹;XIAO Langtao¹;LIAO Bohan¹;LU Xudong^1,2;KUANG Fengchun¹;ZHAO Wenkui¹;TONG Jianhua¹ . Effects of lead stress on anti-oxidative enzyme activities and isoenzymes in cucumber seedlings[J]. Chinese Journal of Applied Ecology.

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