植物种群研究中的分子标记及其应用

摘要/Abstract

摘要： 综述了植物种群分子生态研究中各种标记法及其应用,包括等位酶、AFLP、RAPD、SSR及RFLP等方法.并根据所涉及的有关研究论述及研究工作总结比较了各种方法应用在植物种群学研究中的利弊.强调应依不同的研究目的采用不同的分子标记方法,各种标记方法对解决不同的问题在质和量上是不同的.

关键词: 分子标记, 植物种群, AFLP, RAPD RFLP SSR

Abstract: This review surveys kinds of markers such as allozyme, AFLP, RAPD, SSR, RFLP and their application in molecular ecology of plant populations. The advantages and disadvantages of these molecular markers in plant population research are discussed based on the comparison of the methods and the experience in research work. It is considered emphatically that the adoption of the molecular markers is dependent on the various research purposes and different quality and quantity of the molecular information are acquired from various molecular marker methods.

Key words: Molecular markers, Plant population, AFLP, RAPD, RFLP, SSR

张军丽, 王峥峰, 李鸣光, 王伯荪. 植物种群研究中的分子标记及其应用[J]. 应用生态学报, 2000, (4): 631-636.

ZHANG Junli, WANG Zhengfeng, LI Mingguang, WANG Bosun. Molecular markers and their application in plant population research[J]. Chinese Journal of Applied Ecology, 2000, (4): 631-636.

参考文献

1. Allard RW, Kahler AL, Clegg MT. 1975. Isozymes in plant population genetics. In: Markert CL ed. Isozymes. Ⅳ Genetics and Evolution. New York: Academic Press. 261～272
2. Beismann H, Barker JH, Karp A et al. 1997. AFLP analysis sheds light on distribution of two Salix species and their hybrid along a natural gradient. Mol Ecol,6: 989～993
3. Brown AHD,Moran GF. 1981. Isozymes and the genetic resources of forest trees. In: Conkle MT ed. Proc. of the Symposium on Isozyme of North American Forest Trees and Insects. Berkeley, California: USDA Forest Service General Technical Report PSW, 1～10
4. Cano RJ, Poinar HN, Pieniazek NJ et al. 1993. Amplification and sequencing of DNA from a 120-135-million-year-old weevil. Nature, 363:536～538
5. Chen XL,Lim SH, Lee YH et al. 1998. The study of orchid (aranda) hybrid by AFLP. Eighteenth International Congress of Genetics. Beijing:Sciemce Press.
6. Chen X-Y(陈小勇), Wang X-H(王希华), Song Y-C(宋永昌). 1997. Genetic diversity and differentiation of Cyclobalanopsis glauca populations in east China. Acta Bot Sin(植物学报),39(2): 149～155 (in Chinese)
7. Clegg MT, Brown AHD, and Whitfield PR. 1984. Chloroplast DNA diversity in wild and cultivated barley: Implications for genetic conservation. Genet Res Camb,43: 339～343
8. Crawford FJ. 1983. Phylogenetic and systematic inferences from electrophoretic studies. In: Tanksley SD, and Orton TJ eds. Isozymes in Plant Genetics and Breeding, Part A,Amsterdam: Elsevier Science Publishers BV. 257～287
9. Dow BD, Ashley MV. 1996. Microsatellite analysis of seed dispersal and parentage of samplings in bur oak Quercus macrocarpa. Mol Ecol,5: 615～627
10. Ge S(葛颂), Wang H-Q(王海群), Zhang C-M(张灿明) et al. 1997. Genetic diversity and population differentiation of Cathaya argyrophylla in Bamian Mountain. Acta Bot Sin(植物学报),39(3): 266～271 (in Chinese)
11. Gillies ACM, Cornelius JP, Newton AC et al. 1997. Genetic variation in Costa Rican populations of the tropical timber species Cedrela odorata L. Assessed using RAPDs. Mol Ecol. 6: 1133～1145
12. Hamrick JL, Linhart YB, Mitton JB. 1979. Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. Annu Rev Ecol Syst, 10:173～200
13. Hamrick JL, Godt MJW. 1990. Allozyme diversity in plant species. In: Brown A H D, Clegg MT, Kahler AL, Weir BS eds. Plant Population Genetics, Breeding and Genetic Resources, Sinauer, Sunderland: Massachusetts Press. 43～63
14. Hamrick JL,Murawski DA. 1991. Levels of allozyme diversity in populations of uncommon neotropical tree species. J Trop Ecol, 7: 395～399
15. Hamrick JL. 1987. Gene flow and distribution of genetic variation in plant populations. In: Urbanska K ed. Differentiation Patterns in Higher Plants. New York: Academic Press. 53～67
16. Hoey MT,Parks CR. 1991. Intercontinental patterns of genetic divergence in Asian, North American and Turkish species of Liquidambar (Hamamelidaceae). Amer J Bot,98: 938～947
17. Hu Z-A(胡志昂), Wang H-X(王洪新). 1985. Genetic structure of wild soybean natural populations in Beijing areas. Acta Bot Sin(植物学报),27(6): 599～604 (in Chinese)
18. Isagi Y, Suhandono S. 1997. PCR primers amplifying microsatellite loci of Quercus myrsinifolia Blume and their conservation between oak species. Mol Ecol, 6: 897～901
19. Lerceteau E, Szmidt AE. 1998. QTL mapping wood production and wood quality traits in Pinus sylvestris L. Using AFLP markers. In: Eighteenth International Congress of Genetics, Beijing Acad Press. 176, 8P240
20. Li J-Q(李俊清). 1996. Studies on intra- and inter- species gene diversity of Chinese beeches. Chin Biodiver(生物多样性),4(2): 63～68 (in Chinese)
21. Li J(李军), Qian B(钱波), Zheng S-Zh(郑师章) et al. 1998. Genetic diversity at isozyme level in seed bank of Glycine soja-A preliminary study. Chin J Appl Ecol(应用生态学报),9 (2): 145～149 (in Chinese)
22. Li R-G(李汝刚). 1997. Application of RAPD markers in identification of apple cultivars. Biotechnol Bull(生物技术通报),6:17～21 (in Chinese)
23. Li WH, Luo CC, Wu CI. 1985. Evolution of DNA sequences. In: Molecular Evolutionary Genetics. New York: Academic Press. 1～92
24. Loveless MD, Hamrick JL. 1984. Ecological determinants of genetic structure in plant populations. Annu Rev Ecol Syst,15: 65～95
25. Lynch M, Milligan BG. 1994. Analysis of population genetic structure with RAPD markers. Mol Ecol,3: 91～99
26. Mashburn SJ, Sharitz RR, Smith MH. 1978. Genetic variation among Typha population of the southeastern United States. Evolution, 32: 681～685
27. Merrell D. 1981. Ecological Genetics. (2nd ed). Cambridge: Mass Press.
28. Miller JC, Ranksley SD. 1990. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet,80:437～448
29. Murphy RW. 1993. The phylogenetic analysis of allozyme data:invalidity of coding alleles by presence/absence and recommended procedures. Biochem Syst Ecol,21:25～38
30. Nei M. 1987. Molecular Evolutionary Genetics. New York: Columbia University Press.
31 Nevo E,Beiles A, Ben-Shlomi R. 1984. The evolutionary significance ofgenetic diversity ecological, demographic and live history correlates. In Mani GS ed. Evolutionary Dynamics of Genetic Diversity Lecture Notes in Biomathematics,53: 13～213
32 Nienhuis J, Helentjaris T, Slocum M et al. 1987. Restricton fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci,27:797～803
33 Parker PG, Snow AA, Schug MD et al. 1998. What molecules can tell us about populations: choosing and using a molecular marker. Ecol,79(2): 361～382
34 Peng Y-D(彭源东), Zhang Zh-Zh(张忠泽). 1998. RAPD study on genetic diversity of Frankia. Chin J Appl Ecol (应用生态学报),9(1): 59～63 (in Chinese)
35 Powell JR. 1994. Molecular techniques in population genetics: A brief history. In: Schierwater B, Streit B, Wagner GP and DeSalle R eds. Mol Ecol and Evolution: Approaches and Applications. Berlin: Birhauser Verlag Basel. 131～156
36 Russell JR, Fuller JD, Macaulay M et al. 1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet, 93: 714～722
37 Schaal BA, O'Kane SL, Rogstad SH. 1991. DNA variation in plant populatons. Tree, 6: 329～333
38 Schierenbeck KA, Skupski M, Leiberman D et al. 1997 Population structure and genetic diversity in four tropical tree species from Costa Rica. Mol Ecol. 6: 137～144
39 Soltis PS,Soltis DE,Wolf PG. 1990. Allozymic divergence in North American Polystichum (Dryopteridaceae). Syst Bot,15(2): 205～215
40 Szmid AE, Wang XR, Lu MZ. 1996. Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis. Heredity, 76: 412～420
41 Vos P,Hogers R, Bleeker M, Reijans M et al. 1995. AFLP: anew technique for DNA fingerprinting. Nucl Acids Res. 23: 4407～4414
42 Wachira FN, Waugh R, Jckett CA, Powell W 1995 Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome, 38: 201～210
43 Wang BS, Li MG, Wang ZF, Zhang JL. 1999. AFLP analysis of Cryptocarya concinna population in lower subtropical forests, China. XVI International Botanical Congress Abstract, St. Louis, New York: Academic Press. 1389
44 Wang H-X(王洪新), Hu Z-A(胡志昂), Zhong M(钟敏) et al. 1997. Genetic differentiation and physiological adaptation of wild soybean (Glycine soja) populations under saline conditions: iaozymatic and random amplified polymorphic DNA study. Acta Bot Sin(植物学报). 39(1): 34～42 (in Chinese)
45 Wang M(汪敏), Qian J(钱吉), Zheng S-Z(郑师章). 1998. Isozymes of different geographic populations of Leymus chinensis. Chin J Appl Ecol (应用生态学报),9(3): 269～272 (in Chinese)
46 Wang X-Q(汪小荃), Zou Y-P(邹俞平), Zhang D-M(张大明) et al. 1996a. RAPD analysis of genetic diversity in Cathaya argyrophylla. Sci China (中国科学)26(5): 436～442 (in Chinese)
47 Wang XR, Szmidt AE, Lindgren D. 1991. Allozyme differentiaiton among populations of Pinus sylvestris L. from Sweden and China. Hereditas,114: 219～226
48 Wang Zh-F(王峥峰)et al. 2000. Molecular ecology of Schima superba and Cryptocarya chinensis populations in different commutities.. Acta Bot Sin(植物学报)(in press)
49 Wang Zh-F(王峥峰), Zhang J-L(张军丽), Wang B-S(王伯荪) et al. 2000. Molecular ecology of Cryptocarya chinesis populations in different communities. Chin J Appl Ecol (应用生态学报), 11(3):342～344 (in Chinese).
50 Wang Zh-R(王中仁). 1996. Analysis of Plant Allozyme Beijing: Scientific Press. (in Chinese)
51 Weining S, Langridge P. 1991. Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor Appl Genet, 82: 209～216
52 Weissenbach J,Gyapay G, Dib C et al. 1992 A second-generation linkage map of the human genome. Nature,359: 794～801
53 Wheeler NC,Guries RP. 1987. A quantitative measure of introgression in lodgepole and jack pines. Can J Bot,65:1876～1885
54 Williams JGK, Kubelik AR, Livak KJ, Rafakski JL, Tingey SV. 1990. DNA polymorphism amplified by arbitrary primers are as genetic markers. Nucl Acids Res,18: 6531～6535
55 Yang Y-P(杨一平), Yin R-X(尹瑞雪), Zhang J-L(张军丽). 1991. Genetic diversity of isozymes in natural populations of Pinus sylvestriformis. J Northeast For Univ(东北林业大学学报), 19(5): 17～25 (in Chinese)
56 Yeh FC and Arnott JT. 1986. Electrophoretic and morphological differentiation of Picea sitchensis, Picea glauca and their hybrids. Can J For Res, 16:791～798
57 Zabeau M, Vos P. 1993. Selective restriction fragment amplifivation: a general method for DNA fingerprinting. European Patent, 0535 858A1, 03～31
58 Zhang J-L(张军丽), Yang Y-P(杨一平), Yin R-X(尹瑞雪). 1994. Genetic analysis of Picea koraiensis naturaln population in Changbai Mountain using isozyme electrophoresis. Res For Ecosy(森林生态系统研究), 7: 86～90 (in Chinese)
59 Zhang J-L(张军丽), Wang Z-F(王峥峰),Wang B-S(王伯荪)et al. 2000. AFLP analysis of Crypyocarya concinna, a dominant population in lower subtropical evergreen broad-leaved forests at Heishiding Natural Reserve. Acta Ecol Sin (生态学报)(in press, in Chinese)

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	0

摘要

最新录用	在线预览	正式出版

0	0	0

	来源

	次数
	比例

[1]	魏丹丹,石俊霞,张夏瑄,陈世春,魏冬,王进军**. 基于转录组数据的桔小实蝇微卫星位点信息分析 [J]. 应用生态学报, 2014, 25(6): 1799-1805.
[2]	王鹤潼1，2,金峰1,江奕君3,林青山4,徐海1,陈温福1,徐正进1**. 不同生态条件下水稻籼粳交后代亚种分化机制 [J]. 应用生态学报, 2013, 24(11): 3123-3130.
[3]	赵冰**,徐曼,司国臣,李厚华,张延龙. 秦岭秀雅杜鹃野生种群遗传多样性和遗传分化的AFLP分析 [J]. 应用生态学报, 2012, 23(11): 2983-2990.
[4]	李新凤1,张光明1,畅引东1,王建明1**,李蕊倩2,李亚立1. 21株马特组镰刀菌遗传多样性的ISSR分析 [J]. 应用生态学报, 2012, 23(05): 1339-1344.
[5]	陈名君,黄勃,刘玉军,李增智. 马尾松纯林中球孢白僵菌种群的遗传异质性 [J]. 应用生态学报, 2010, 21(10): 2627-2634.
[6]	郑文明¹;康振生²;蒋士君³;陈长卿². 小麦条锈菌分子生态学研究进展 [J]. 应用生态学报, 2008, 19(03): 681-685 .
[7]	余仲东;曹支敏;原犇犇. 松杨栅锈菌遗传多样性初步分析 [J]. 应用生态学报, 2006, 17(11): 2102-2106 .
[8]	张军丽, 王峥峰, 王伯荪, 李鸣光, 张炜银. 鹤山人工林大叶相思种群遗传结构的AFLP分析 [J]. 应用生态学报, 2001, 12(4): 491-495.
[9]	张军丽, 王峥峰, 王伯荪, 李鸣光, 张炜银. 鹤山人工林大叶相思种群遗传结构的AFLP分析 [J]. 应用生态学报, 2001, (4): 491-495.
[10]	张军丽, 王峥峰, 李鸣光, 王伯荪. 植物种群研究中的分子标记及其应用 [J]. 应用生态学报, 2000, (4): 631-636.
[11]	王峥峰, 张军丽, 王伯荪, 李鸣光. 厚壳桂种群在不同群落中的分子生态研究 [J]. 应用生态学报, 2000, (3): 342-344.
[12]	王峥峰, 张军丽, 王伯荪, 李鸣光. 厚壳桂种群在不同群落中的分子生态研究 [J]. 应用生态学报, 2000, (3): 342-344.