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氯化镉对葡萄根系线粒体特性与根系活力的影响

邵小杰1,2;杨洪强1,2;乔海涛2;张龙2;由淑贞2   

  1. 1山东农业大学作物生物学国家重点实验室, 山东泰安 271018;2山东农业大学园艺科学与工程学院, 山东泰安 271018
  • 收稿日期:2008-11-17 修回日期:1900-01-01 出版日期:2009-06-20 发布日期:2009-06-20

Effects of CdCl2 on grape root mitochondrial characteristics and root activity.

SHAO Xiao-jie1,2;YANG Hong-qiang1,2;QIAO Hai-tao2;ZHANG Long2;YOU Shu-zhen2   

  1. 1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, China;2College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2008-11-17 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20

摘要: 以巨峰、玫瑰香、龙眼和泽香4个葡萄品种一年生扦插苗为试材,研究了氯化镉(CdCl2)处理后葡萄根系线粒体过氧化氢(H2O2)、膜通透性转换孔(MPTP)、膜电位(Δψ)、细胞色素C(Cyt c)及根系活力的变化.结果表明:4个葡萄品种经0.5 mmol·L-1的CdCl2处理后,根系线粒体H2O2含量上升,MPTP开放程度增大,Δψ降低,Cyt c含量下降,根系活力明显降低;其中,H2O2含量和Cyt c 含量由高到低的顺序为:巨峰>泽香>玫瑰香>龙眼,而根系活力、MPTP和Δψ由高到低的顺序为:龙眼>玫瑰香>泽香>巨峰.表明巨峰葡萄根系活力比其他品种更易受到CdCl2的影响,而龙眼葡萄根系活力在4个品种中受CdCl2影响最小.

关键词: 径向基函数神经网络模型, 普通克里格法, 回归克里格法, 土壤养分

Abstract: Taking the one-year cutting-seedlings of four grape cultivars (Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondr ial hydrogen peroxide (H2O2) content, membrane permeability transition pore (MPTP), membrane potential (Δψ), cytochrome C (Cyt c) content, and root activity were measured under effects of CdCl2. For all test grape cultivars, the root mitochondrial H2O2 content and MPTP increased, but the Δψ, Cyt c content, and root activity decreased after treated with 05 mmol CdCl2·L-1. The m itochondrial H2O2 content and Cyt c content were in the sequence of Kyoho>Ze Xiang>Muscat Hamburg>Long Yan, while the root activity, MPTP, and Δψ were in ad verse, i.e., Long Yan>Muscat Hamburg>Ze Xiang>Kyoho. Among the test grape cultiv ars, the root activity of Kyoho was easier to be inhibited under Cd stress, while t hat of Long Yan was least affected by CdCl2.

Key words: radial basis function neural network model, ordinary kriging, regression kriging, soil nutrient.