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不同耕作方式下稻田土壤的氮素形态及氮素转化菌特征

顾明华1;区惠平1;刘昔辉2;黄景1;黎晓峰1;李柳霞1;沈方科1;江立庚1   

  1. 1广西大学农学院, 南宁 530005;2广西甘蔗研究所, 南宁 530007
  • 收稿日期:2008-11-17 修回日期:1900-01-01 出版日期:2009-06-20 发布日期:2009-06-20

Characteristics of N forms and N-transforming bacteria in paddy soil under different tillage patterns.

GU Ming-hua1;OU Hui-ping1;LIU Xi-hui2;HUANG Jing1;LI Xiao-feng1;LI Liu-xia1;SHEN Fang-ke1;JIANG Li-geng1   

  1. 1College of Agronomy, Guangxi University, Nanning 530005, China;2Guangxi Sugarcane Research Institute, Nanning 530007, China
  • Received:2008-11-17 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20

摘要: 在水稻不同生育期分层采集稻田土壤剖面样品,研究不同耕作方式(常耕CT、免耕NT、稻草还田常耕CTS、稻草还田免耕NTS)、不同土层稻田土壤的氮素(N)形态及氮素转化菌特征.结果表明:在N素转化菌方面,水稻整个生育期0~5 cm土层氨化细菌的数量以NTS处理最多;0~5 cm和5~12 cm土层亚硝化细菌数量CT处理高于NT处理,12~20 cm土层则相反;NTS较CTS处理降低了0~20 cm土层的亚硝化细菌和反硝化细菌数量;在水稻拔节期和成熟期,NT处理较CT处理提高了0~5 cm土层的嫌气性固氮菌数量.在N素形态方面,水稻整个生育期NT处理碱解N和全N较集中分布在0~5 cm土层,明显高于CT处理,而5~12 cm和12~20 cm土层比CT处理低;12~20 cm土层的铵态氮和硝态氮含量NT与CT处理差异不显著,而NTS处理0~20 cm土层的铵态氮和硝态氮含量均有所提高.相关分析和多元回归分析表明,铵态氮与氨化细菌、亚硝化细菌、反硝化细菌正相关程度最高,而碱解氮与嫌气性固氮菌正相关程度最高,均达极显著水平.综合各土层氮素转化菌数量与不同形态N含量,NTS更有利于稻田氮素供应与养分积累.

关键词: 番茄, 光合诱导, 气孔调节, 水分胁迫

Abstract: Paddy soil samples were collected in layers (0〖KG-*2〗-〖KG-*7〗5, 5〖KG-*2〗- 〖KG-*7〗12, and 12〖KG-*2〗-〖KG-*7〗20 cm) during rice growth period to invest igate the characteristics of the N forms and N-transforming bacteria in the soi l profile under different tillage patterns (no-tillage with straw returning, NT S; conventional tillage with straw returning, CTS; no-tillage, NT; and conventi onal tillage, CT). In the whole rice growth period, ammonifying bacteria in 0〖K G-*2〗-〖KG-*7〗5 cm soil layer had the highest number under NTS, and nitrosobac teria in 0〖KG-*2〗-〖KG-*7〗5 cm and 5〖KG-*2〗-〖KG-*7〗12 cm soil layers were more abundant but in 12〖KG-*2〗-〖KG-*7〗20 cm soil layer were lesser under CT than under NT. Nitrosobacteria and denitrobacteria in 0〖KG-*2〗-〖KG-*7〗20 cm soil layer were lesser under NTS than under CTS. At elongating and ripening sta ges, anaerobic N-fixing bacteria in 0〖KG-*2〗-〖KG-*7〗5 cm soil layer were mo re abundant under NT than under CT. In the whole rice growth period, the alkali -hydrolyzable N and total N contents in 0〖KG-*2〗-〖KG-*7〗5 cm soil layer wer e significantly higher but in 5〖KG-*2〗-〖KG-*7〗12 cm and 12〖KG-*2〗-〖KG-*7 〗20 cm soil layers were lower under NT than under CT, and the NH4+-N and N O3--N contents in 0〖KG-*2〗-〖KG-*7〗20 cm soil layer were higher under NT S but in 12〖KG-*2〗-〖KG-*7〗20 cm soil layer had no significant differences be tween NT and CT. Correlation analysis and multiple polynomial regression analysi s further revealed that there were significant relationships between soil NH4 +-N and soil ammonifying bacteria, nitrosobacteria and denitrobacteria, and b etween soil alkali-hydrolyzable N and soil anaerobic N-fixing bacteria. Among the test tillage patterns, NTS could be the more desirable one for the N supply and fertility maintenance of paddy soil.

Key words: tomato, photosynthetic induction, stomatal regulation, water stress.