产紫篮状菌的生防潜力及其对土壤微生物群落的调控

doi:10.13287/j.1001-9332.202010.037

应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3255-3266.doi: 10.13287/j.1001-9332.202010.037

产紫篮状菌的生防潜力及其对土壤微生物群落的调控

田叶韩, 彭海莹, 王德浩, 李晓芳, 何邦令, 高克祥^*

山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室, 山东泰安 271000

收稿日期:2019-12-30 接受日期:2020-07-16 出版日期:2020-10-15 发布日期:2021-04-15
通讯作者: * E-mail: kxgao63@163.com
作者简介:田叶韩, 男, 1991年生, 博士研究生. 主要从事土传病害综合治理技术的研究. E-mail: tianyehan@163.com
基金资助:
国家公益性行业(农业)科研专项(201503110-12)、山东省重大科技创新工程项目(2017CXGC0207,2019JZZY010714)和山东省重点研发计划项目(2018GNC111005)资助

Biocontrol potential of Talaromyces purpurogenus and its regulation on soil microbial community

TIAN Ye-han, PENG Hai-ying, WANG De-hao, LI Xiao-fang, HE Bang-ling, GAO Ke-xiang^*

College of Plant Protection, Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Tai'an 271000, Shandong, China

Received:2019-12-30 Accepted:2020-07-16 Online:2020-10-15 Published:2021-04-15
Contact: * E-mail: kxgao63@163.com
Supported by:
Special Fund for Agro-Scientific Research in the Public Interest of China (201503110-12), the Key Scientific Innovation Program of Shandong Province (2017CXGC0207, 2019JZZY010714), and the Key Research and Deve-lopment Program of Shandong Province (2018GNC111005).

摘要/Abstract

摘要： 产紫篮状菌Q2菌株是一株分离自健康黄瓜根际的有益微生物。本文通过平皿对峙培养、温室盆栽试验和田间试验评估了Q2菌株对4种土传病害的生防潜力及其与土壤熏蒸技术结合对苦瓜枯萎病的防治效果,并通过平皿稀释培养法、高通量技术和定量PCR技术对其防治苦瓜枯萎病及调控土壤微生物群落的机制进行研究。结果表明: 在温室条件下,Q2菌株对苦瓜枯萎病、烟草黑胫病、烟草根黑腐病和马铃薯茎基腐病具有明显的预防效果,对烟草黑胫病和苦瓜枯萎病防治效果分别达到75.3%和63.4%。在苦瓜枯萎病人工病圃中,单一产紫篮状菌Q2制剂对苦瓜枯萎病的防治效果为51.0%,其结合威百亩土壤熏蒸技术在相同试验条件下对枯萎病的防治效果则达到80%以上。威百亩土壤熏蒸显著降低了土壤中苦瓜枯萎病病原菌即尖镰孢菌的丰度,而Q2菌株有效地抑制了尖镰孢菌数量的恢复趋势。施用Q2菌株显著富集了土壤中的青霉菌、芽孢杆菌和Gaiella等有益微生物,抑制了尖镰孢菌的恢复。土壤熏蒸后,施用产紫篮状菌Q2菌株有助于土壤有益微生物菌群的形成,从而抑制尖镰孢菌,实现对苦瓜枯萎病的防治。

关键词: 产紫篮状菌, 生防微生物, 土壤传播病害, 微生物群落结构, 高通量测序

Abstract: Talaromyces purpurogenus strain Q2 is a kind of beneficial microbe originally separated from the rhizosphere of healthy cucumber. In this study, we evaluated the biocontrol potential of strain Q2 against four soil-borne diseases by plate confrontation culture, potting in the greenhouse. We further estimated the control efficacy of strain Q2 combined metam-sodium fumigation against Fusarium wilt of bitter gourd in the field. The mechanism of strain Q2 controlling bitter gourd wilt and regulating soil microbial community was examined by plate dilution culture, high throughput sequencing and quantitative PCR. The results showed that strain Q2 could efficiently reduce disease incidence of Fusarium wilt of bitter gourd, potato stem canker, black shank of tobacco and black root rot of tobacco in the green house. Its biocontrol efficacy on black shank of tobacco and bitter gourd wilt was 75.3% and 63.4%, respectively. Biocontrol efficacy of strain Q2 on bitter gourd wilt was 51.0% in the artificial disease nursery inoculated pathogen of bitter gourd wilt, while the control efficacy of strain Q2 combined with soil fumigation technology was more than 80% in the same experiment condition. Strain Q2 application and soil fumigation altered soil microbial community composition and recovery trend. Metam-sodium fumigation significantly reduced the abundances of Fusarium oxysporum and causal agent of bitter gourd wilt. Strain Q2 further suppressed the efficient recovery trend of the pathogen. After application of strain Q2, Penicillium was enriched in soil, as well as the beneficial microbes involved in the suppression of F. oxysporum, such as Bacillus and Gaiella. Overall, after soil fumigation, biocontrol efficacy of strain Q2 on soil-borne diseases such as Fusarium wilt could attribute to the formation of beneficial microbial communities in soil and inhibition of strain Q2 on growth and development of F. oxysporum.

Key words: Talaromyces purpurogenus, biocontrol agent, soil-borne disease, microbial community structure, high throughput sequencing

田叶韩, 彭海莹, 王德浩, 李晓芳, 何邦令, 高克祥. 产紫篮状菌的生防潜力及其对土壤微生物群落的调控[J]. 应用生态学报, 2020, 31(10): 3255-3266.

TIAN Ye-han, PENG Hai-ying, WANG De-hao, LI Xiao-fang, HE Bang-ling, GAO Ke-xiang. Biocontrol potential of Talaromyces purpurogenus and its regulation on soil microbial community[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3255-3266.

参考文献

[1] 杨亚东, 王志敏, 曾昭海. 长期施肥和灌溉对土壤细菌数量、多样性和群落结构的影响. 中国农业科学, 2018, 51(2): 290-301 [Yang Y-D, Wang Z-M, Zeng Z-H. Effects of long-term different fertilization and irrigation managements on soil bacterial abundance, diversity and composition. Scientia Agricultura Sinica, 2018, 51(2): 290-301]
[2] Kennedy AC, Smith KL. Soil microbial diversity and the sustainability of agricultural soils. Plant and Soil, 1995, 170: 75-86
[3] 黄新琦, 蔡祖聪. 土壤微生物与作物土传病害控制. 中国科学院院刊, 2017, 32(6): 593-600 [Huang X-Q, Cai Z-C. Soil microorganism and soil borne disease control of crops. Bulletin of Chinese Academy of Sciences, 2017, 32(6): 593-600]
[4] 李天来, 杨丽娟. 作物连作障碍的克服——难解的问题. 中国农业科学, 2016, 49(5): 916-918 [Li T-L, Yang L-J. Overcoming continuous cropping obstacles—The difficult problem. Scientia Agricultura Sinica, 2016, 49(5): 916-918]
[5] 姚小东, 李孝刚, 丁昌峰, 等. 连作和轮作模式下花生土壤微生物群落不同微域分布特征.土壤学报, 2019, 56(4): 975-985 [Yao X-D, Li X-G, Ding C-F, et al. Microzone distribution characteristics of soil microbial community with peanut cropping system, monocropping or rotation. Acta Pedologica Sinica, 2019, 56(4): 975-985]
[6] 王小兵, 骆永明, 李振高, 等. 长期定位施肥对红壤地区连作花生生物学性状和土传病害发生率的影响. 土壤学报, 2011, 48(4): 725-730 [Wang X-B, Luo Y-M, Li Z-G, et al. Effects of long-term stationary fertilization experiment on incidence of soil-borne diseases and biological characteristics of peanut in continuous monocropping system in red soil area. Acta Pedologica Sinica, 2011, 48(4): 725-730]
[7] 沈宗专, 孙莉, 王东升, 等. 石灰碳铵熏蒸与施用生物有机肥对连作黄瓜和西瓜枯萎病及生物量的影响. 应用生态学报, 2017, 28(10): 3351-3359 [Shen Z-Z, Sun L, Wang D-S, et al. Effects of lime-ammonium bicarbonate fumigation and biofertilizer application on Fusarium wilt and biomass of continuous cropping cucumber and watermelon. Chinese Journal of Applied Ecology, 2017, 28(10): 3351-3359]
[8] 刘海洋, 姚举, 张仁福, 等. 黄萎病不同发生程度棉田中土壤微生物多样性. 生态学报, 2018, 38(5): 1619-1629 [Liu H-Y, Yao J, Zhang R-F, et al. Verticillium wilt has different degrees of soil microbial diversity in cotton fields. Acta Ecologica Sinica, 2018, 38(5): 1619-1629]
[9] 马艳, 胡安忆, 杨豪, 等. 菜粕生物熏蒸防控辣椒疫病. 中国农业科学, 2013, 46(22): 4698-4706 [Ma Y, Hu A-Y, Yang H, et al. Effects of biofumigation with rapeseed meal on disease control of Phytophthora blight of chilli pepper. Scientia Agricultura Sinica, 2013, 46(22): 4698-4706]
[10] 张洁, 夏明聪, 刘红彦, 等. 低剂量棉隆熏蒸联合生物菌肥防治黄瓜根结线虫病的应用效果. 植物保护学报, 2019, 46(4): 824-831 [Zhang J, Xia M-C, Liu H-Y, et al. Efficacy of dazomet fumigation and bioorganic fertilizer in integrated control of cucumber root-knot. Journal of Plant Protection, 2019, 46(4): 824-831]
[11] Mohamad O, Li L, Ma JB, et al. Evaluation of the antimicrobial Activity of endophytic bacterial populations from Chinese traditional medicinal plant licorice and characterization of the bioactive secondary metabolites produced by Bacillus atrophaeus against Verticillium dahliae. Frontiers in Microbiology, 2018, 9: 924
[12] Castillo AG, Puig CG, Cumagun C. Non-synergistic effect of Trichoderma harzianum and Glomus spp. in reducing infection of Fusarium wilt in banana. Pathogens, 2019, 8: 43, doi: 10.3390/pathogens8020043
[13] Abbasi S, Safaie N, Sadeghi A, et al. Streptomyces strains induce resistance to Fusarium oxysporum f. sp. lycopersici race 3 in tomato through different molecular mechanisms. Frontiers in Microbiology, 2019, 10: 1505, doi: 10.3389/fmicb.2019.01505
[14] Jiang CH, Yao XF, Mi DD, et al. Comparative transcriptome analysis reveals the biocontrol mechanism of Bacillus velezensis F21 against Fusarium wilt on watermelon. Frontiers in Microbiology, 2019, 10: 652, doi: 10.3389/fmicb.2019.00652
[15] Shi L, Du NS, Shu S, et al. Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community. Scientific Reports, 2017, 7: 41234, doi: 10.1038/srep41234
[16] Chen ZD, Huang RK, Li QQ, et al. Development of pathogenicity and AFLP to characterize Fusarium oxysporum f. sp. momordicae isolates from bitter gourd in China. Journal of Phytopathology, 2015, 163: 202-211
[17] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 烟草病虫害分级及调查方法(GB/T 23222—2008). 北京: 中国标准出版社, 2008 [General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, National Standardization Administration of China. Grade and Investigation Method of Tobacco Disease (GB/T 23222-2008). Beijing: China Standards Press, 2008]
[18] Weinhold AR. Rhizoctonia disease of potato: Effect on yield and control by seed tuber treatment. Plant Disease, 1982, 66: 815, doi: 10.1094/pd-66-815
[19] 高克祥, 田叶韩, 刘晓光, 等. 一株防治苦瓜枯萎病的拮抗真菌及其应用, 中国, ZL201610959637.2. 2016-10-27 [Gao K-X, Tian Y-H, Liu X-G, et al. An antagonistic fungi against Fusarium wilt of bitter gourd and its application. China, ZL201610959637.2. 2016-10-27]
[20] Mardones W, Callegari E, Eyzaguirre J. Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum. Mycology, 2018, 10: 118-125
[21] 牛玉静, 曹红, 黄晓林, 等. 复合诱变Penicillium purpurogenum Li-3选育高产红色素及其生物活性研究. 中国酿造, 2017, 36(3): 30-34 [Niu Y-J, Cao H, Huang X-L, et al. Screening of high red pigment-producing Penicillium purpurogenum Li-3 by compound mutagenese and its biological activity. China Brewing, 2017, 36(3): 30-34]
[22] Santos-Ebinuma VC, Roberto IC, Simas TMF, et al. Improving of red colorants production by a new Penicillium purpurogenum strain in submerged culture and the effect of different parameters in their stability. Biotechnology Progress, 2013, 29: 778-785
[23] Dita M, Barquero M, Heck D, et al. Fusarium wilt of banana: Current knowledge on epidemiology and research needs toward sustainable disease management. Frontiers in Plant Science, 2018, 9: 1468, https://doi: org/10.3389/fpls.2018.01468
[24] Li XG, Zhang YN, Ding CF, et al. Declined soil suppressiveness to Fusarium oxysporum by rhizosphere microflora of cotton in soil sickness. Biology and Fertility of Soils, 2015, 51: 935-946
[25] Shen ZZ, Penton CR, Lyu NN, et al. Banana Fusarium wilt disease incidence is influenced by shifts of soil microbial communities under different monoculture spans. Microbial Ecology, 2017, 75: 739-750
[26] Garbeva P, Van Veen JA, Van Elsas JD. Microbial diversity in soil: Selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 2004, 42: 243-270
[27] Shen ZZ, Xue C, Penton CR, et al. Suppression of banana Panama disease induced by soil microbiome reconstruction through an integrated agricultural strategy. Soil Biology and Biochemistry, 2018, 128: 164-174
[28] 贲海燕, 石延霞, 谢学文, 等. 氰氨化钙土壤消毒对黄瓜根腐病及土壤病原菌的控制效果. 园艺学报, 2016, 43(11): 2173-2181 [Ben H-Y, Shi Y-X, Xie X-W, et al.Studies of soil improvement effect of calcium cyanamide and its control efficiency on soil-borne diseases of vegetable crops. Acta Horticulturae Sinica, 2016, 43(11): 2173-2181]
[29] 卜东欣, 张超, 张鑫, 等. 熏蒸剂威百亩对土壤微生物数量和酶活性的影响. 中国农学通报, 2014, 30(15): 227-233 [Bu D-X, Zhang C, Zhang X, et al. Effects of fumigant metham-sodium on soil microbial population and enzyme activities. Chinese Agricultural Science Bulletin, 2014, 30(15): 227-233]

产紫篮状菌的生防潜力及其对土壤微生物群落的调控

Biocontrol potential of Talaromyces purpurogenus and its regulation on soil microbial community

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郭蓉, 吴旭东, 王占军, 蒋齐, 俞鸿千, 贺婧, 刘文娟, 马琨. 荒漠草原土壤细菌和真菌群落对降水变化的响应 [J]. 应用生态学报, 2023, 34(6): 1500-1508.
[2]	丁爽, 魏圣钊, 陈真亮, 邵婧, 段逢瑞, 严禹, 段兴武. 中国西南典型森林土壤微生物在不同土壤深度下的变化特征 [J]. 应用生态学报, 2023, 34(3): 614-622.
[3]	陶新, 夏世斌, 刘秋梅, 李德军, 何寻阳. 甘蔗黑穗病的微生物防治研究进展 [J]. 应用生态学报, 2023, 34(3): 846-852.
[4]	江尚焘, 栗晗, 彭海英, 梅新兰, 陈廷速, 徐阳春, 董彩霞, 沈其荣. 有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响 [J]. 应用生态学报, 2023, 34(2): 481-490.
[5]	刘艳娇, 刘庆, 贺合亮, 赵文强, 寇涌苹. 亚高山粗枝云杉人工林土壤原核微生物群落结构与功能变化 [J]. 应用生态学报, 2023, 34(12): 3279-3290.
[6]	王星, 杨腾, 毛子昆, 蔺菲, 叶吉, 房帅, 戴冠华, 胡家瑞, 郝占庆, 王绪高, 原作强. 长白山阔叶红松林优势树种叶际真菌群落结构 [J]. 应用生态学报, 2022, 33(9): 2405-2412.
[7]	高慧芳, 孟婷, 熊琦, 章鸿宇, 邱君志, 林文雄, 张燎原. 太子参不同休耕年限土壤理化特征和微生物群落变化 [J]. 应用生态学报, 2022, 33(8): 2196-2204.
[8]	杨贵森, 张志山, 赵洋, 石亚飞, 虎瑞. 沙坡头地区凋落物分解及其对土壤微生物群落的影响 [J]. 应用生态学报, 2022, 33(7): 1810-1818.
[9]	王晓菲, 罗珠珠, 张仁陟, 牛伊宁, 李玲玲, 田建霞, 孙鹏洲, 刘家鹤. 黄土高原苜蓿-粮食作物轮作下土壤细菌群落特征和生态功能预测 [J]. 应用生态学报, 2022, 33(4): 1109-1117.
[10]	杜贞娜, 单之初, 沈赤, 程斐, 郭怀宇, 臧威, 孙剑秋, 潘兴祥. 传统绍兴黄酒酿造真菌群落与多样性发生机制 [J]. 应用生态学报, 2022, 33(4): 1118-1124.
[11]	陈冰冰, 孙志高, 胡星云, 武慧慧, 王晓颖, 毛立, 厉彦哲. 闽江河口互花米草不同入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性 [J]. 应用生态学报, 2022, 33(11): 3007-3015.
[12]	田建霞, 罗珠珠, 李玲玲, 牛伊宁, 蔡立群, 刘家鹤, 孙鹏洲, 王晓菲. 陇中黄土高原半干旱区不同种植年限紫花苜蓿土壤线虫群落分布特征 [J]. 应用生态学报, 2022, 33(10): 2829-2835.
[13]	杨虎, 马巧蓉, 杨君珑, 周亮, 曹兵, 张维江. 宁夏南部生态移民迁出区不同恢复模式土壤微生物群落特征 [J]. 应用生态学报, 2022, 33(1): 219-228.
[14]	肖意, 陈慧娴, 邱丽君, 张扬, 万松泽. 林下植物剔除对毛竹林土壤微生物群落结构的影响 [J]. 应用生态学报, 2021, 32(9): 3089-3096.
[15]	杨雅丽, 马雪松, 解宏图, 鲍雪莲, 梁超, 朱雪峰, 何红波, 张旭东. 保护性耕作对土壤微生物群落及其介导的碳循环功能的影响 [J]. 应用生态学报, 2021, 32(8): 2675-2684.