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应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3547-3553.doi: 10.13287/j.1001-9332.202010.036

• 研究论文 • 上一篇    下一篇

木屑添加对刺芹侧耳菌丝共生细菌多样性和群落结构的影响

许欣, 柳婷, 孔旭强, 陈剑秋, 陈利丁, 黄胜, 孙淑静*   

  1. 福建农林大学生命科学学院, 福州 350002
  • 收稿日期:2020-02-01 接受日期:2020-07-17 出版日期:2020-10-15 发布日期:2021-04-15
  • 通讯作者: * E-mail: shjsun2004@126.com
  • 作者简介:许 欣, 男, 1995年生, 硕士研究生. 主要从事食用菌与细菌互作研究. E-mail: 1282549120@qq.com
  • 基金资助:
    国家自然科学基金项目(31801556)、福建省区域发展项目(2019N3018)、福建省杰出青年项目(2014J06010)和福建农林大学专项基金项目(CXZX2017397,CXZX2017505)资助

Effects of sawdust dosage on the diversity and community structure of bacteria associated with the hyphae of Pleurotus eryngii

XU Xin, LIU Ting, KONG Xu-qiang, CHEN Jian-qiu, CHEN Li-ding, HUANG Sheng, SUN Shu-jing*   

  1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2020-02-01 Accepted:2020-07-17 Online:2020-10-15 Published:2021-04-15
  • Contact: * E-mail: shjsun2004@126.com
  • Supported by:
    National Natural Science Foundation of China (31801556), the District Development Program of Fujian Province (2019N3018), the Natural Science Foundation of Fujian Province for Distinguished Young Scholar (2014J06010), and the Specific Fund of Fujian Agriculture and Forestry University (CXZX2017397,CXZX2017505).

摘要: 细菌作为许多真菌的共生菌,能够有效地促进真菌的代谢生长,而细菌多样性及群落结构能够反映真菌的生长和利用营养物质的状况。本研究利用基于细菌16S rRNA 基因V3-V4区的高通量测序技术分析不同木屑用量对刺芹侧耳菌丝共生细菌群落结构和多样性的影响。结果表明: 5组样品共检测细菌25门52纲114目199科406属,主要的优势菌门为变形菌门(35.0%~85.9%)和厚壁菌门(6.5%~38.4%),优势菌属为不动杆菌属(14.8%~71.6%)和假单胞菌属(1.7%~22.3%)。相较于完全培养基,添加木屑能够提高刺芹侧耳菌丝中的细菌多样性,并使其中优势细菌10个门类、9个属类结构发生显著变化。刺芹侧耳菌丝在5 g木屑培养基上的菌丝生长速度最快,菌丝浓密,边缘整齐,长势优于其他几类培养基;假单胞菌属和乳酸菌属丰度及物种多样性在5 g木屑培养基上都具有一定的优势,且假单胞菌属和乳酸菌属丰度与菌丝长势具有显著的正相关性。木屑作为重要碳源之一,对刺芹侧耳生长发育及其共生细菌群落结构和多样性都有显著影响,这为进一步探索木屑及共生细菌对刺芹侧耳生长发育影响的机理研究奠定了基础。

关键词: 刺芹侧耳, 有机碳源, 菌丝生长, 高通量测序, 细菌多样性, 细菌群落结构

Abstract: Some fungi engage symbiosis with bacteria, which can effectively promote the metabolism and growth of fungi. The diversity and community structure of bacteria can reflect the growth and substrate utilization of fungi. In this study, we analyzed the effects of different sawdust dosages on the community structure and diversity of bacteria associated with the hyphae of Pleurotus eryngii using high-throughput sequencing technology (HST) based on PCR-amplified 16S rRNA V3-V4 fragments. The results showed that the high-quality sequences from five groups of mycelia samples were clustered into 25 phyla, 52 classes, 114 orders, 199 families, and 406 genera. Proteobacteria (35.0%-85.9%) and Firmicutes (6.5%-38.4%) were the most abundant bacterial phyla, while Acinetobacter (14.8%-71.6%) and Pseudomonas (1.7%-22.3%) were the dominant symbiotic genera. Compared with the mycelia grown on the complete culture medium, sawdust addition could increase the diversity of bacteria coexisting with P. eryngii mycelia, and change the community structure of 10 dominant phyla and 9 dominant genera. P. eryngii cultivated in substrate containing 5 g sawdust had the fastest mycelium growth rate, thick mycelia, and neatly edges. Furthermore, judging from the abundance and diversity, Pseudomonas and Lactobacillus became the dominant genera, which were positively correlated with the mycelia growth vigour. Sawdust, as an important carbon source, could affect the growth and development of P. eryngii and the community structure and diversity of bacteria coexisting with mycelia. This study would lay a theoretical foundation for exploring the molecular mechanism of sawdust and mycelium symbiosis affecting the growth and development of P. eryngii.

Key words: Pleurotus eryngii, organic carbon source, mycelial growth, high-throughput sequencing, bacterial diversity, bacterial community structure