多功能植物根际促生菌对东北黑土区玉米的促生效果

doi:10.13287/j.1001-9332.202008.036

应用生态学报 ›› 2020, Vol. 31 ›› Issue (8): 2759-2766.doi: 10.13287/j.1001-9332.202008.036

多功能植物根际促生菌对东北黑土区玉米的促生效果

陈腊¹, 米国华², 李可可¹, 邵慧², 胡栋³, 杨俊鹏¹, 隋新华^1*, 陈文新¹

¹中国农业大学生物学院/农业部土壤微生物学重点实验室, 北京 100193;
²中国农业大学资源与环境学院/植物-土壤相互作用教育部重点实验室, 北京 100193;
³河北省农林科学院遗传生理研究所, 石家庄 050051

收稿日期:2019-12-04 修回日期:2020-04-24 出版日期:2020-08-15 发布日期:2021-02-15
通讯作者: * E-mail: suixh@cau.edu.cn
作者简介:陈腊, 男, 1993年生, 博士研究生。主要从事环境微生物与农业生态研究。E-mail: 819431881@qq.com
基金资助:
国家重点研发计划项目(2017YFD0201801)资助

Effects of multifunctional plant rhizosphere promoting bacteria on maize growth in black soil areas in Northeast China

CHEN La¹, MI Guo-hua², LI Ke-ke¹, SHAO Hui², HU Dong³, YANG Jun-peng¹, SUI Xin-hua^1*, CHEN Wen-xin¹

¹College of Biological Sciences, China Agricultural University/Key Laboratory of Soil Microbiology, Ministry of Agriculture, Beijing 100193, China;
²College of Resources and Environmental Science, China Agricultural University/Key Laboratory of Plant-Soil Interaction, Ministry of Education, Beijing 100193, China;
³Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received:2019-12-04 Revised:2020-04-24 Online:2020-08-15 Published:2021-02-15
Supported by:
This work was supported by the National Key R&D Program of China (2017YFD0201801).

摘要/Abstract

摘要： 应用微生物肥料改良土壤是提高土壤及化肥养分利用率的重要途径之一,但微生物肥料的效果受作物及生态条件的影响较大,目前适合东北地区玉米生产的微生物肥料应用研究较少。本研究针对我国东北黑土类型及气候特点,从当地玉米根际土中筛选分离出5株具有不同程度的合成生长素(IAA)、溶磷、解钾、产铁载体等多功能的植物根际促生菌MZ1、MZ2、MZ3、MZ4和MZ5;生态适应性研究发现5株菌均有不同程度的耐盐、耐干旱、耐酸碱以及耐农药等特性;16S rRNA基因序列分析鉴定出它们分别属于鞘氨醇单胞菌、肠杆菌、假单胞菌、芽孢杆菌和根瘤菌;玉米田间试验结果显示,在减施50%氮肥的条件下, 与不接种对照相比,接种MZ1、MZ3和MZ5可增产19.9%~25.0%。因此,MZ1、MZ3和MZ5具有发展为适合东北地区土壤和气候环境的玉米微生物肥料的潜力。

关键词: 东北地区, 黑土, 玉米, 植物根际促生菌, 生态适应性, 促生

Abstract: The application of microbial fertilizer plays an important role in improving soil restoration and fertilizer utilization. The effects of microbial fertilizer are greatly affected by crop genotypes and ecological conditions. Little is known about the effects of microbial fertilizers on maize production in Northeast China. To develop microbial fertilizer specific to the black soil and the climate characteristics of Northeast China, we isolated five plant rhizosphere-promoting bacteria (PGPR), named as MZ1, MZ2, MZ3, MZ4 and MZ5, with different degrees of biological functions such as IAA synthesis, phosphate-solubilizing, potassium-solubilizing and siderophore-releasing, from the rhizosphere of maize field. The analysis of ecological adaptability showed that those five strains differed in salt resistance, drought tolerance, acid and alkali resistance, pesticide resistance. The 16S rRNA gene sequences analysis showed that the strains MZ1, MZ2, MZ3, MZ4 and MZ5 belonged to the genus of Sphingomonas, Enterobacter, Pseudomonas, Bacillus and Rhizobium, respectively. In maize field experiment with 50% nitrogen fertilizer reduction, the inoculation with MZ1, MZ3 and MZ5 increased grain yield by 19.9%-25.0%. MZ1, MZ3, and MZ5 could be used as microbial fertilizers for maize in Northeast China.

Key words: Northeast China, black soil, maize, PGPR, ecological adaptability, growth promoting

陈腊, 米国华, 李可可, 邵慧, 胡栋, 杨俊鹏, 隋新华, 陈文新. 多功能植物根际促生菌对东北黑土区玉米的促生效果[J]. 应用生态学报, 2020, 31(8): 2759-2766.

CHEN La, MI Guo-hua, LI Ke-ke, SHAO Hui, HU Dong, YANG Jun-peng, SUI Xin-hua, CHEN Wen-xin. Effects of multifunctional plant rhizosphere promoting bacteria on maize growth in black soil areas in Northeast China[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2759-2766.

参考文献

[1] Xu XZ, Xu Y, Chen SC, et al. Soil loss and conservation in the black soil region of Northeast China: A retrospective study. Environmental Science & Policy, 2010, 13: 793-800
[2] Yu H, Gao Q, Shao Z, et al. Decreasing nitrogen ferti-lizer input had little effect on microbial communities in three types of soils. PLoS One, 2016, 11(3): e0151622, doi: 10.1371/journal.pone.0151622
[3] Guo JH, Liu XJ, Zhang Y, et al. Significant acidification in major Chinese croplands. Science, 2010, 327: 1008-1010
[4] Zhou J, Guan D, Zhou B, et al. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biology and Biochemistry, 2015, 90: 42-51
[5] Mahanty T, Bhattacharjee S, Goswami M, et al. Biofertilizers: A potential approach for sustainable agriculture development. Environmental Science and Pollution Research, 2017, 24: 3315-3335
[6] 李俊, 姜昕, 马鸣超, 等. 我国微生物肥料产业需求与技术创新. 中国土壤与肥料, 2019(2): 1-5 [Li J, Jiang X, Ma M-C, et al. Development demand and technical innovation for bio-fertilizer industry in China. Soil and Fertilizer Sciences in China, 2019(2): 1-5]
[7] Vessey JK. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 2003, 255: 571-586
[8] Kloepper JW, Schroth MN. Plant growth-promoting rhizobacteria on radishes. Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Angers, 1978: 879-882
[9] Bardi L, Malus E. Drought and nutritional stresses in plant: Alleviating role of rhizospheric microorganisms// Haryana N, Punj S, eds. Abiotic Stress: New Research. Hauppauge, NY, USA: Nova Science Publishers Inc., 2012: 1-57
[10] Malusa E, Vassilev N. A contribution to set a legal framework for biofertilisers. Applied Microbiology and Biotechnology, 2014, 98: 6599-6607
[11] Adesemoye AO, Torbert HA, Kloepper JW. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology, 2009, 58: 921-929
[12] 胡江春, 薛德林, 马成新, 等. 植物根际促生菌(PGPR)的研究与应用前景. 应用生态学报, 2004, 15(10): 1963-1966 [Hu J-C, Xue D-L, Ma C-X, et al.Research advances in plant growth-promoting rhizobacteria and its application prospects. Chinese Journal of Applied Ecology, 2004, 15(10): 1963-1966]
[13] 王素英, 陶光灿, 谢光辉, 等. 我国微生物肥料的应用研究进展. 中国农业大学学报, 2013, 8(1): 14-18 [Wang S-Y, Tao G-C, Xie G-H, et al. A review of effects of biofertilizers on crop yield and quality. Journal of China Agricultural University, 2013, 8(1): 14-18]
[14] Cassn F, Diaz-Zorita M. Azospirillum sp. in current agriculture: From the laboratory to the field. Soil Biology and Biochemistry, 2016, 103: 117-130
[15] 荣良燕, 姚拓, 黄高宝, 等. 植物根际优良促生菌(PGPR)筛选及其接种剂部分替代化肥对玉米生长影响研究. 干旱地区农业研究, 2013, 31(2): 59-65 [Rong L-Y, Yao T, Huang G-B, et al. Screening of plant growth promoting rhizobacteria strains and effects of inoculant on growth of maize by replacing part of chemical fertilizers. Agricultural Research in the Arid Areas, 2013, 31(2): 59-65]
[16] 陈龙, 孙广正, 姚拓, 等. 干旱区微生物肥料替代部分化肥对玉米生长及土壤微生物的影响. 干旱区资源与环境, 2016, 30(7): 108-113 [Chen L, Sun G-Z, Yao T, et al. Effect of chemical fertilizer partly replaced by microbial fertilizer on maize growth and soil microorganism in arid area. Journal of Arid Land Resources and Environment, 2016, 30(7): 108-113]
[17] 孙淑荣, 吴海燕, 刘春光, 等. PGPR复合制剂对玉米增产效果的研究. 吉林农业科学, 2004, 29(5): 22-28 [Sun S-R, Wu H-Y, Liu C-G, et al. Study on the effect of PGPR complex microbial community on maize yield. Journal of Jilin Agricultural Sciences, 2004, 29(5): 22-28]
[18] Vacheron J, Desbrosses G, Bouffaud ML, et al. Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science, 2013, 4: 356, doi: 10.3389/fpls.2013.00356
[19] 余悦. 黄河三角洲原生演替中土壤微生物多样性及其与土壤理化性质关系. 博士论文. 济南: 山东大学, 2012 [Yu Y. Relationships between Soil Microbial Diversity and Soil Physicochemical Properties along the Primary Succession of Yellow River Delta. PhD Thesis. Ji'nan: Shandong University, 2012]
[20] Pikovskaya RI. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiology, 1948, 17: 362-370
[21] 林启美, 赵小蓉, 孙焱鑫, 等. 四种不同生态系统的土壤解磷细菌数量及种群分布. 土壤与环境, 2000, 9(1): 34-37 [Lin Q-M, Zhao X-R, Sun Y-X, et al.Community characters of soil phosphobacteria in four ecosystems. Soil and Environmental Sciences, 2000, 9(1): 34-37]
[22] Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 1987, 160: 47-56
[23] 刘晓璐, 刘永智, 郭涛, 等. 解钾细菌的筛选、鉴定以及高效培养. 北京科技大学学报, 2013, 35(4): 139-145 [Liu X-L, Liu Y-Z, Guo T, et al. Isolation, identification and high-efficiency cultivation of potassium-releasing bacteria. Journal of University of Science and Technology Beijing, 2013, 35(4): 139-145]
[24] Glickmann E, Dessaux YA. Critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 1995, 61: 793-796
[25] 吴红萍, 万红艳, 王锐萍. 戊唑醇农药降解菌的筛选及其降解效能初探. 农药, 2013, 52(2): 102-104 [Wu H-P, Wan H-Y, Wang R-P. Tebuconazole pesticide degradation bacteria screening and its degradation efficiency trial. Agrochemicals, 2013, 52(2): 102-104]
[26] 王金生, 王君, 吴俊江, 等. 基于GGE-biplot的大豆根瘤菌抗逆性资源筛选. 大豆科学, 2017, 36(6): 894-899 [Wang J-S, Wang J, Wu J-J, et al. Screening the resistance resources of Rhizobium japonicum based on GGE-biplot. Soybean Science, 2017, 36(6): 894-899]
[27] 廖婷婷, 翟磊, 高成华, 等. 一株产甘露聚糖酶菌株的分离鉴定及酶的纯化与性质. 微生物学报, 2011, 51(11): 1520-1526 [Liao T-T, Zhai L, Gao C-H, et al. Purification and characterization of mannanase from an alkaliphilic mannanase producing bacterium HMTS15. Acta Microbiologica Sinica, 2011, 51(11): 1520-1526]
[28] 万兵兵, 刘晔, 吴越, 等. 一株玉米根际多功能促生菌的筛选鉴定及效应研究. 生物技术通报, 2016, 32(8): 169-176 [Wan B-B, Liu Y, Wu Y, et al. Screening and identification of maize growth-promoting rhizobacteria and its promoting effects on maize. Biotechnology Bulletin, 2016, 32(8): 169-176]
[29] 姚拓. 高寒地区燕麦根际联合固氮菌研究: Ⅱ. 固氮菌的溶磷性和分泌植物生长素特性测定. 草业学报, 2004, 13(3): 85-90 [Yao T. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region: Ⅱ. Phosphate-solubilizing power and auxin production. Acta Prataculturae Sinica, 2004, 13(3): 85-90]
[30] 荣良燕, 姚拓, 赵桂琴, 等. 产铁载体 PGPR 菌筛选及其对病原菌的拮抗作用. 植物保护, 2011, 37(1): 59-64 [Rong L-Y, Yao T, Zhao G-Q, et al. Screening of siderophore-producing PGPR bacteria and their anta-gonism against the pathogens. Plant Protection, 2011, 37(1): 59-64]
[31] 陈强, 陈文新, 张小平, 等. 四川省部分豆科植物根瘤菌的遗传多样性. 应用与环境生物学报, 2008, 14(1): 83-89 [Chen Q, Chen W-X, Zhang X-P, et al. Genetic diversity of rhizobia isolated from some legumes in Sichuan, China. Chinese Journal of Applied and Environmental Biology, 2008, 14(1): 83-89]
[32] Lane DJ. 16S/23S rRNA Sequencing// Stackebrandt E, Goodfellow M, eds. Nucleic Acid Techniques in Bacte-rial Systematics. Chichester, UK: John Wiley and Sons, 1991: 115-175
[33] Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016, 33: 1870-1874
[34] 于玲玲, 郭强, 赵贵元. 保护性耕作对华北地区玉米产量及水分利用效率的影响. 内蒙古农业大学学报, 2017, 38(3): 19-23 [Yu L-L, Guo Q, Zhao G-Y. The effects of conservation tillage on maize yield and water use efficiency in North China. Journal of Inner Mongolia Agricultural University, 2017, 38(3): 19-23]
[35] 陈延玲. 协调玉米高产与氮高效转运的机制. 博士论文. 北京:中国农业大学, 2015 [Chen Y-L. Mechanisms for the Coordination of High Yield and High Nitrogen Remobilization Efficiency in Maize. PhD Thesis. Beijing: China Agricultural University, 2015]
[36] Cassn F, Perrig D, Sgroy V, et al. Corrigendum to “Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.) [Eur. J. Soil Biol. 45 (2009) 28-35]”. European Journal of Soil Biology, 2011, 47: 1, doi: 10.1016/j.ejsobi.2011.05.003
[37] Díaz-Zorita M, Grove J. Wheat grain response to nitrogen fertilization and field inoculation with a liquid formulation of Azospirillum brasilense. ASA-CSSA-SSSA International Annual Meetings. Indianapolis, IN, USA, 2006: 25031
[38] 康贻军, 程洁, 梅丽娟, 等. 植物根际促生菌作用机制研究进展. 应用生态学报, 2010, 21(1): 232-238 [Kang Y-J, Cheng J, Mei L-J, et al. Action mechanisms of plant growth-promoting rhizobacteria (PGPR): A review. Chinese Journal of Applied Ecology, 2010, 21(1): 232-238]
[39] Dubeikovsky AN, Mordukhova EA, Kochetkov VV, et al. Growth promotion of blackcurrant softwood cuttings by recombinant strain Pseudomonas fluorescens bsp53a synthesizing an increased amount of indole-3-acetic acid. Soil Biology and Biochemistry, 1993, 25: 1277-1281
[40] Mi GH, Chen FJ, Yuan LX, et al. Ideotype root system architecture for maize to achieve high yield and resource use efficiency in intensive cropping systems. Advances in Agronomy, 2016, 139: 73-97
[41] Haas D, Défago G. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Reviews Microbiology, 2005, 3: 307-319
[42] Barzanti R, Ozino F, Bazzicalupo M, et al. Isolation and characterization of endophytic bacteria from the nickel hyperaccumulator plant Alyssum bertolonii. Microbial Ecology, 2007, 53: 306-316
[43] 曹宁, 符力, 张玉斌, 等. 低温对玉米苗期根系生长及磷养分吸收的影响. 玉米科学, 2008, 16(4): 58-60 [Cao N, Fu L, Zhang Y-B, et al. Effects of low temperature on root of maize seedling growth and phosphorus uptake. Journal of Maize Sciences, 2008, 16(4): 58-60]
[44] Whitelaw MA, Harden TJ, Helyar KR. Phosphate solubilisation in solution culture by the soil fungus Penicil-lium radicum. Soil Biology and Biochemistry, 1999, 31: 655-665

多功能植物根际促生菌对东北黑土区玉米的促生效果

Effects of multifunctional plant rhizosphere promoting bacteria on maize growth in black soil areas in Northeast China

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