基于稳定同位素技术的增殖放流牙鲆群体鉴别

doi:10.13287/j.1001-9332.202201.023

应用生态学报 ›› 2022, Vol. 33 ›› Issue (1): 261-267.doi: 10.13287/j.1001-9332.202201.023

基于稳定同位素技术的增殖放流牙鲆群体鉴别

王青林^1,2,3, 于姗姗^1,2, 靳晓敏^1,2*, 任建功⁴, 司飞⁴, 孙桂清^3,5, 孙朝徽⁴, 白曾齐¹

¹河北科技师范学院, 海洋资源与环境学院, 河北秦皇岛 066000;
²河北省海洋动力过程与资源环境重点实验室, 河北秦皇岛 066000;
³河北省海洋生物与资源重点实验室, 河北秦皇岛 066000;
⁴中国水产科学研究院北戴河中心实验站, 河北秦皇岛 066000;
⁵河北省海洋与水产科学研究院, 河北秦皇岛 066000

收稿日期:2021-04-29 接受日期:2021-10-31 出版日期:2022-01-15 发布日期:2022-07-15
通讯作者: * E-mail: xicom@163.com
作者简介:王青林, 男, 1984年生, 博士。主要从事水产增养殖研究。E-mail: wangqinglin_1984@163.com
基金资助:
国家自然科学基金项目(31702350)、河北科技师范学院博士研究启动基金项目(2019YB018)和河北科技师范学院省属高校基本科研业务费专项(2020JK013)

Identification of released population of Japanese flounder based on stable isotopes analysis

WANG Qing-lin^1,2,3, YU Shan-shan^1,2, JIN Xiao-min^1,2*, REN Jian-gong⁴, SI Fei⁴, SUN Gui-qing^3,5, SUN Zhao-hui⁴, BAI Zeng-qi¹

¹College of Marine Resources & Environment, Hebei Normal University of Science & Technology, Qinhuangdao 066000, Hebei, China;
²Hebei Key Laboratory for Ocean Dynamics, Resources and Environments, Qinhuangdao 066000, Hebei, China;
³Marine Living Resources and Environment Key Laboratory of Hebei Province, Qinhuangdao 066000, Hebei, China;
⁴Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066000, Hebei, China;
⁵Ocean Fisheries Science Research Institute of Hebei Province, Qinhuangdao 066000, Hebei, China

Received:2021-04-29 Accepted:2021-10-31 Online:2022-01-15 Published:2022-07-15

摘要/Abstract

摘要： 牙鲆是我国黄渤海重要的增殖放流鱼类,野生群体和放流群体的鉴别是评估牙鲆增殖效果的前提。为了研究稳定同位素技术在增殖放流牙鲆群体鉴别中的应用,本研究以秦皇岛近海增殖放流区捕捞牙鲆幼鱼为对象,先使用项目组前期开发的形态学和分子标记相结合的方法区分野生群体和放流群体,再分别测定肌肉碳、氮稳定同位素值和耳石(全耳石和耳石核心区域)碳、氧稳定同位素值,并以养殖群体为对照。结果表明: 利用肌肉δ¹³C值(野生群体:-17.19‰±0.73‰;放流群体:-17.10‰±0.61‰;养殖群体:-20.75‰±0.07‰)和δ¹⁵N值(野生群体:11.81‰±0.38‰;放流群体:11.62‰±0.48‰;养殖群体:8.64‰±0.60‰)及全耳石中δ¹³C值(野生群体:-4.47‰±0.35‰;放流群体:-4.63‰±0.29‰;养殖群体:-6.59‰±0.58‰)可鉴别出养殖群体,但是无法区分野生群体和放流群体;利用耳石核心区δ¹³C值(野生群体:-4.66‰±0.30‰;放流群体:-5.41‰±0.21‰;养殖群体:-5.37‰±0.19‰)可鉴别出野生群体。3个群体全耳石和耳石核心区δ¹⁸O值均有重叠,无法对群体进行鉴别。表明利用耳石核心区δ¹³C值可鉴别重建牙鲆幼鱼群体中的野生群体和放流群体,并在生殖洄游亲鱼群体的鉴别方面有一定的应用前景,这为后续估计增殖放流对牙鲆早期资源量的补充及深入开展增殖放流效果评估工作提供了基础数据和技术手段。

关键词: 稳定同位素, 牙鲆, 野生群体, 放流群体, 养殖群体, 耳石, 鉴别

Abstract: Japanese flounder (Paralichthys olivaceus) is an important releasing fish in the Yellow and Bohai Seas of China. The identification of wild and releasing population is the premise to evaluate the enhancement effects. In order to study the application of stable isotope in the identification of released P. olivaceus population, captured juveniles in the offshore releasing area of Qinhuangdao were distinguished into wild and released population using previous method (combination of morphology and molecular). Then, the contents of carbon and nitrogen stable isotope in muscle and otoliths (including the whole and the core region) were measured. The cultured population was set as control. The results showed that δ¹³C values (wild population: -17.19‰±0.73‰; released population: -17.10‰±0.61‰; cultured population: -20.75‰±0.07‰) and δ¹⁵N values (wild population: 11.81‰±0.38‰; released population: 11.62‰±0.48‰; cultured population: 8.64‰±0.60‰) of muscle and δ¹³C value (wild popu-lation: -4.47‰±0.35‰; released population: -4.63‰±0.29‰; cultured population: -6.59‰±0.58‰) of the whole otolith could be used to identify the cultured population, but could not be used to distinguish the wild from the released population. The δ¹³C value (wild population: -4.66‰±0.30‰; released population: -5.41‰±0.21‰; cultured population: -5.37‰±0.19‰) of the core region of otolith could be used to identify the wild popu-lation. The δ¹⁸O values of the whole and the core region of otolith from these three groups were overlapped and could not be used to distinguish different populations. Our results indicated that the δ¹³C value of the core area of otolith could be used to identify wild and released population, with application prospect in the identification of broodstocks participating in spawning migration. This study provided basic data and technical methods for evaluating early resources replenishment and the effects of Japanese flounder enhancement.

Key words: stable isotope, Japanese flounder, wild population, released population, cultured population, otolith, identification

王青林, 于姗姗, 靳晓敏, 任建功, 司飞, 孙桂清, 孙朝徽, 白曾齐. 基于稳定同位素技术的增殖放流牙鲆群体鉴别[J]. 应用生态学报, 2022, 33(1): 261-267.

WANG Qing-lin, YU Shan-shan, JIN Xiao-min, REN Jian-gong, SI Fei, SUN Gui-qing, SUN Zhao-hui, BAI Zeng-qi. Identification of released population of Japanese flounder based on stable isotopes analysis[J]. Chinese Journal of Applied Ecology, 2022, 33(1): 261-267.

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基于稳定同位素技术的增殖放流牙鲆群体鉴别

Identification of released population of Japanese flounder based on stable isotopes analysis

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