温州蜜柑叶片光系统反应中心光能分配的变化

应用生态学报 ›› 2004, Vol. ›› Issue (11): 2087-2090.

温州蜜柑叶片光系统反应中心光能分配的变化

郭延平¹, 宋丽丽², 徐凯^1,2, 张良诚¹

1. 浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室, 杭州 310029;
2. 安徽农业大学园艺系, 合肥 230036

收稿日期:2003-09-08 修回日期:2004-05-02 出版日期:2004-11-15
通讯作者: 郭延平,男,1963年生,博士后,副教授,主要从事果树生理与环境生物学研究,发表论文30多篇.E-mail:guoyping@mail.hz.zj.cn.
基金资助:
国家自然科学基金项目(39970525);浙江省自然科学基金资助项目(399470)

Changes of light energy distribution in reaction centers of Citrus unshiu leaf photosystem under different light intensities

GUO Yanping¹, SONG Lili², XU Kai^1,2, ZHANG Liangcheng ¹

1. State Agriculture Ministry Laboratory of Horticultural Plant Growth Development and Biotechnology, Department of Horticulture, Zhejiang University, Hangzhou 310029, China;
2. Department of Horticulture, Anhui Agricultural University, Hefei 230036, China

Received:2003-09-08 Revised:2004-05-02 Online:2004-11-15

摘要/Abstract

摘要： 为深入了解果树光化学反应中心光能分配的状况,以柑橘为试材,采用调制荧光法对叶片光系统在高光强和低光强下的状态转换进行了研究.结果表明, 光系统在100 μmol·m^-2·s^-1的低光强下,由于QA的还原使PQ库处于还原状态,导致光能由PSⅡ转向PSⅠ分配,光系统处于状态2;在1 000 μmol·m^-2·s^-1 的高光强下, PQ库无法得到电子而处于氧化状态,导致光能分配由PSⅠ转向PSⅡ,光系统处于状态1.叶片经磷酸酯酶抑制剂NaF处理后,光系统从高光强下状态2到状态1的转换受到抑制.高光强下过多的光能由PSⅠ向PSⅡ分配是导致PSⅡ光破坏的重要原因.

关键词: 温州蜜柑, 光强, 叶绿素荧光, 状态转换, PQ库

Abstract: In order to further understand the light energy distribution in photochemical reaction centers of fruit trees,this paper employed modulated fluorescence technique to study the state transition of photosystem in Citrus unshill leaves under high and low light intensities.The results indicated that under low light intensity of 100 μmol·m^-2·s^-1,the PQ pool was in reduction state due to Q_A reduction,which made the light energy distribution in photosystem changed from PSⅡ to PSⅠ; while under high light intensity of 1 000 μmol·m^-2·s^-1,the PQ pool could not get electron and was in oxidation state,making light energy distribution changed from state 2 to state 1.When the leaves were treated by a phosphatase inhibitor NaF,the state 2 to state 1 transition of photosystem was inhibited under high light intensity,and the excessive light energy distribution from PSⅠ to PSⅡ caused damage to PSⅡ.These findings would be helpful to understand the mechanisms of the photoinhibition of photosynthesis and the photodamage of photosynthetic apparatus of fruit trees.

Key words: Citrus unshiu, Light intensity, Chlorophyll fluorescence, State transition, PQ pool

中图分类号:

郭延平, 宋丽丽, 徐凯, 张良诚. 温州蜜柑叶片光系统反应中心光能分配的变化[J]. 应用生态学报, 2004, (11): 2087-2090.

GUO Yanping, SONG Lili, XU Kai, ZHANG Liangcheng . Changes of light energy distribution in reaction centers of Citrus unshiu leaf photosystem under different light intensities[J]. Chinese Journal of Applied Ecology, 2004, (11): 2087-2090.

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