珍稀濒危植物天目木兰(Magnolia amoena)遗传多样性的RAPD分析

应用生态学报 ›› 2004, Vol. ›› Issue (7): 1139-1142.

珍稀濒危植物天目木兰(Magnolia amoena)遗传多样性的RAPD分析

刘登义, 储玲, 杨月红

安徽师范大学生物多样性中心, 重要生物资源保护与利用研究安徽省重点实验室, 芜湖 241000

收稿日期:2003-10-08 修回日期:2004-04-18 出版日期:2004-07-15
通讯作者: 刘登义,男,1958年9月生,博士,教授,博导;主要从事植物种群生态学、生物多样性与保护生物学和环境生态学研究,发表论文60余篇,出版专著两部.Tel:0553-3869233;E-mail:ldy@mail.ahnu.edu.cn
基金资助:
安徽省自然科学基金项目(03043501);教育部科学技术重点研究项目;重要生物资源保护与利用研究安徽省重点实验室专项基金资助项目

Genetic diversity of rare and endangered plant Magnolia amoena

LIU Dengyi, CHU Ling, YANG Yuehong

Biodiversity Research Center, The Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources in Anhui, Anhui Normal University, Wuhu 241000, China

Received:2003-10-08 Revised:2004-04-18 Online:2004-07-15

摘要/Abstract

摘要： 运用随机扩增多态DNA(RAPD)技术,对天目木兰(Magnolia amoena)居群的遗传多样性进行了研究.从40个10-mer随机引物中筛选出14个能得到清晰、稳定扩增带的引物进行扩增,14个引物共检测了94个位点,其中多态性位点为23,占24.4%.计算了12个居群之间的遗传相似度和遗传距离,并运用UPGMA法进行了聚类分析,结果显示相同产地个体间(居群内)的遗传距离较小,遗传多样性水平很低;不同产地个体间(居群间)遗传距离较大,遗传多样性水平较前者高,即天目木兰个体间遗传多样性水平与它的地理分布有关.天目木兰总体较低的遗传多样性是导致它濒危的原因之一.

关键词: 天目木兰, 聚类分析, RAPD

Abstract: The genetic variations within and between eleven natural populations and one cultured population of Magnolia amoena from different distribution regions were investigated at the DNA level by employing RAPD.Out of 40 random primers,fourteen random primers were screened which could generate highly reproducible and clear RAPD fragments for further population analysis.With these primers,a total of 94 discernible DNA fragments were obtained and 23(24.4%) were polymorphic,which indicated that low levels of genetic variation existed in the investigated populations.In addition,UPGMA map were made according to the genetic similarity and distance of the twelve populations calculated in this article.The result showed that the genetic diversity between individuals within population was lower than that between populations.The individual genetic differentiation might be relation to their geographic distribution.The low total genetic diversity of Magnolia amoena was one reason for its endangerment.

Key words: Magnolia amoena, Cluster analysis, RAPD

中图分类号:

Q948.3

刘登义, 储玲, 杨月红. 珍稀濒危植物天目木兰(Magnolia amoena)遗传多样性的RAPD分析[J]. 应用生态学报, 2004, (7): 1139-1142.

LIU Dengyi, CHU Ling, YANG Yuehong. Genetic diversity of rare and endangered plant Magnolia amoena[J]. Chinese Journal of Applied Ecology, 2004, (7): 1139-1142.

参考文献

[1] Bureau of the National Environmental Conservation(国家环境保护总局),Institute of Botany of the Chinese Academy of Sciences(中国科学院植物研究所).1987.Lists of Chinese Rare and Endangered Plants. Beijing: Science Press. (in Chinese)
[2] Caetano-Anolles G. 1993.Amplifying DNA with arbitrary oligonucleotide primers. PCR Methods and Applications, 3: 85 ～ 94
[3] Cai X(蔡霞),Hu Z-H(胡正海).2000.Comparative studies on leaf structure and oil cells of the Magnoliaceaein China.Acta Phytotaxon Sin(植物分类学报),38(3):218～230(in Chinese)
[4] Chen R-Y(陈瑞阳),Zhang W(张玮),Wu Q-A(武全安).1989.A report on chromosome numbers of partly Magnoliaceae plants in Yunnan,China.Acta Bota Yunnanica(云南植物研究),11(2):234～238(in Chinese)
[5] Dawson IK, Chalmers KJ, Waugh R, Powell W. 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israle using RAPD markers. Mol Ecol, 2:151～159
[6] Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 9(1):11 ～ 15
[7] Du D-L (杜道林), Su J (苏洁), et al. Genetic diversity of Cephalotaxus mannii, a rare and endangered plant. Acta Bot Sin(植物学报),44(2):193～198(in Chinese)
[8] Falk DA, Holsinger KE. 1991. Genetics and Conservation of Rare Plants. New York:Oxford University Press Inc.
[9] Fu L-G (傅立国), et al. 1991. China Plant Red Data Book-Rare and Endangered Plants. Volume I.Beijing:Science Press. (in Chinese)
[10] Lanner C, Bryngelsson T, Gustafsson M. 1996. Genetic validity of RAPD markers at the intra-and inter-specific in wild Brassica species with n = 9. Theor Appl Genet, 93: 9～ 14
[11] Liu Y-H(刘玉壶). 1996. Flora Reipublicae Popularis Sinicae. Vol.30. Beijing: Science Press. 82 ～ 198 (in Chinese)
[12] Liu Z-L(刘占林), Zhao G-F(赵桂仿). 1999. Population geneticand its implications for conservation of rare and endangered plants.Chin Biodiver ( 生物多样性), 7 (4): 340～ 346 ( in Chinese)
[13] Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 76: 5269 ～ 5273
[14] Pei Y-L(裴颜龙),Zou Y-P(邹喻苹),et al.1995.Preliminary report of RAPD analysis in Paeonia suffruticosa subsp.sopontanea and Paeonia rockii.Acta Phytotaxon Sin(植物分类学报),33(4):350～356(in Chinese)
[15] Peng S-L(彭少麟),Zhang T-P(张太平).2000.Anintroduction to molecular ecology of cultivated plants.J Wuhan Bot Res(武汉植物研究),18(2):165～168(in Chinese)
[16] Qiao A-M(乔爱民),Liu P-Y(刘佩瑛),Lei J-J(雷建军).1998.RAPD analysis of sixteen varieties ofmustard.Acta BotSin(植物学报),40(10):915～921(in Chinese)
[17] Williams JPK, Kubelik AR, Livak KJ. et al. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucleic Acids Res, 18: 6531～ 6535
[18] Wu S-M(吴树明), Lin J-X(林金星), Li Z-L(李正理). 1993.Comparative studies on wood rays anatomy off sixty-six plant species in nine genera of Magnoliaceae in China. Acta Bot Sin (植物学报), 35(4) :268 ～ 279
[19] Zhang J-Y(张继益), Dong Y-C(董玉琛),Jia Z-Z(贾增增), et al.1999. RAPD analysis for the germplasm resources of genus Eremopyrum. Acta Gene Sin (遗传学报), 26 ( 1 ): 54 ～ 60 (in Chinese)
[20] Zou Y-P(邹喻苹), et al. 2001. Molecular Markers in Systematic and Evolutional Botany. Beijing: Science Press. 34 ～ 35 (in Chinese)