Effects of exogenous melatonin on photosynthesis and physiological characteristics of chry-santhemum seedlings under high temperature stress

doi:10.13287/j.1001-9332.202107.025

Abstract

Abstract: We examined the effects of exogenous melatonin (MT) on the resistance of Chrysanthemum morifolium ‘Jinba' to high temperature stress. Chrysanthemum leaves were sprayed with 200 μmol·L^-1MT, and then subjected to high temperature stress at 40 ℃ (day)/ 35 ℃ (night). The ultrastructure of chloroplast and thylakoid of chrysanthemum leaves were observed, and the photosynthetic and physiological indices were measured. The results showed that the chloroplast and thyla-koid structures of chrysanthemum were damaged under high temperature stress. The chlorophyll contents and maximum fluorescence (F_m) were significantly reduced, while the OJIP curve changed with the fluorescence of K and J points increased. The net photosynthetic rate (P_n), transpiration rate (T_r) and stomatal conductance (g_s) were significantly decreased, while the internal CO₂ concentration (C_i) was significantly increased. The relative conductivity (REC), malondialdehyde (MDA), reactive oxygen species (ROS), osmotic adjustment substances content and antioxidant enzyme activity all increased significantly. Spraying exogenous MT onto leaves could maintain the integrity of chloroplast and thylakoid structure under high temperature in chrysanthemum and significantly decrease the increment in the K and J points of OJIP curve. Exogenous application of MT alleviated the inhibition of high temperature stress on photosynthesis and fluorescence of chrysanthemum, as indicated by significantly higher F_m, P_n, g_s, T_r and photosynthetic pigment contents and lower C_i. Exogenous MT also significantly reduced the REC, MDA and ROS contents of chrysanthemum under high temperature stress, and enhanced the osmotic adjustment substances content and antioxidant enzyme activity in chrysanthemum leaves. It suggested that exogenous MT could protect the integrity of chloroplast structure of chrysanthemum leaves, enhance photosynthesis, inhibit the excessive production of ROS in the plants under high temperature stress, improve the activity of antioxidant enzyme system, reduce the level of membrane peroxidation and keep the integrity of lipid membrane, and thus improve the ability of chrysanthemum plants to resist high temperature stress.

Key words: chrysanthemum, high temperature stress, melatonin, photosynthesis, physiologicalmechanism

QI Xiao-yuan, WANG Wen-li, HU Shao-qing, LIU Meng-yu, ZHENG Cheng-shu, SUN Xian-zhi. Effects of exogenous melatonin on photosynthesis and physiological characteristics of chry-santhemum seedlings under high temperature stress[J]. Chinese Journal of Applied Ecology, 2021, 32(7): 2496-2504.

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