Effects of long-term fertilization on soil ammonia-oxidizing microorganisms

doi:10.13287/j.1001-9332.202005.024

Abstract

Abstract: Long-term fertilization can change the supply of soil carbon and nitrogen (N), with consequences on the abundance and community structure of soil microorganisms. Based on the long-term fertilization positioning experiment station of brown earth, we analyzed the dynamics of soil ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) under different fertilization treatments, including no fertilization (CK), low level of inorganic N fertilizer (N₂), high level of inorganic N fertilizer (N₄), and organic manure combined with inorganic N fertilizer (M₂N₂), aiming to provide a basis for microbiological mechanism of soil N transformation and improvement of soil fertility. The results showed that the ratio of AOA to AOB abundance was 2.28-61.95 under different fertilization treatments. Compared with that in CK, the AOA abundance was reduced by 1.6%-13.6% after long-term fertilization. The abundance of AOB in N₄ treatment decreased first and then increased with soil depths, but with contrary results in other treatments. The Shannon diversity index (H), evenness index (J), and Simpson index (S) of AOB were higher than those of AOA. The AOB diversity was increased at 0-20 cm soil layer in M₂N₂ treatment, while that of AOA was decreased. Soil AOB clustered with soil depths, and neither AOA nor AOB community clustered with fertilization treatments. In summary, long-term fertilization altered the composition of AOA and AOB. AOA was sensitive to environment, whereas AOB was more abundant and stable.

Key words: ammonia-oxidizing archaea, ammonia-oxidizing bacteria, long-term fertilization, diversity

LIU Ling-zhi, MA Shi-han, LI Xiu-ling, AN Ting-ting, WANG Jing-kuan. Effects of long-term fertilization on soil ammonia-oxidizing microorganisms[J]. Chinese Journal of Applied Ecology, 2020, 31(5): 1459-1466.

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