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Biological characteristics of Metarhizium anisopliae var.major and its virulence to white grubs

LIN Huafeng;LI Shiguang;ZHANG Lei;WANG Pingli;ZHOU Yiwen   

  1. School of Plant Protection,Anhui Agricultural University,Hefei 230036,China
  • Received:2005-04-21 Revised:2005-10-20 Online:2006-02-18 Published:2006-02-18

Abstract: With Beauveria brongniartii and Metarhizium anisopliae var.anisopliae as the reference,this paper studied the biological characteristics and invading ability of Metarhizium anisopliae var.major,a new variety separated from white grubs.The results showed that the optimal culture condition of M.anisopliae var.major was similar with that of other entomopathogenic species,and the optimal temperature was 25 ℃.Among the three test media,PPDA was the most appropriate one,followed by SDAY and Czapek.M.anisopliaevar.major had a stronger virulence than B.brongniartii at lower temperature,with an accumulative mortality of white grubs being 88.23% and 76.47%,respectively,after treated with soil and through spraying,suggesting that M.anisopliae var.major had a good prospect to widely apply in field.

Key words: Drought stress, Gene expression, ABA, Signaling transduction, Transgenic plant