外源茉莉酸甲酯诱导抗性与敏感稗草对二氯喹啉酸抗性的差异及机理

doi:10.13287/j.1001-9332.202007.038

应用生态学报 ›› 2020, Vol. 31 ›› Issue (7): 2293-2298.doi: 10.13287/j.1001-9332.202007.038

外源茉莉酸甲酯诱导抗性与敏感稗草对二氯喹啉酸抗性的差异及机理

曹晶晶¹, 彭琼², 杨霞¹, 杨倩¹, 柏连阳², 李永丰^1*, 张自常¹, 谷涛¹

¹江苏省农业科学院植物保护研究所, 南京 210014;
²杂草生物学及安全防控湖南省重点实验室, 长沙 410125

收稿日期:2019-12-31 接受日期:2020-04-18 出版日期:2020-07-15 发布日期:2021-01-15
通讯作者: E-mail: liyongfeng_2010@163.com
作者简介:曹晶晶, 女, 1990年生, 博士。主要从事农田草害研究。E-mail: caojingjing2017@163.com
基金资助:
国家自然科学基金项目(31801773,31672042)和杂草生物学及安全防控湖南省重点实验室开放项目(2015TP1016)资助

Differences in exogenous methyl jasmonate-induced quinclorac resistance between resistant and sensitive barnyardgrass and the underlying mechanism

CAO Jing-jing¹, PENG Qiong², YANG Xia¹, YANG Qian¹, BAI Lian-yang², LI Yong-feng^1*, ZHANG Zi-chang¹, GU Tao¹

¹Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
²Hunan Provincial Key Laboratory for Biology and Control of Weeds, Changsha 410125, China

Received:2019-12-31 Accepted:2020-04-18 Online:2020-07-15 Published:2021-01-15
Contact: E-mail: liyongfeng_2010@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31801773, 31672042) and the Opening Foundation of Hunan Provincial Key Laboratory for Biology and Control of Weeds (2015TP1016).

摘要/Abstract

摘要： 以遗传背景一致的抗二氯喹啉酸型和敏感型稗草为供试材料,分析了经外源茉莉酸甲酯(MeJA)预处理后,稗草种群间应对二氯喹啉酸胁迫的抗性水平及4种内源激素含量的变化。结果表明: 外源MeJA处理可显著提高抗性稗草对二氯喹啉酸的抗性,而对敏感稗草的抗性影响不显著。供试稗草间植株体内生长素(IAA)、脱落酸(ABA)、水杨酸(SA)和茉莉酸(JA)的含量及其变化幅度存在显著差异。二氯喹啉酸处理显著增加了稗草ABA、SA和JA的含量,且敏感稗草ABA和JA含量升高幅度明显高于抗性稗草;外源MeJA预处理能显著增强二氯喹啉酸对稗草ABA和JA含量的诱导,且敏感稗草变化幅度较大。表明敏感稗草较快的激素变化不利于其应对除草剂胁迫,抗性稗草相对平缓的激素变化赋予了其对除草剂的适应性,且MeJA处理增强了其对二氯喹啉酸的抗性。稗草植株体内ABA和JA激素信号在其抵抗二氯喹啉酸胁迫过程中可能发挥重要的生物学功能。

关键词: 稗草, 二氯喹啉酸, 抗药性, 植物激素, 外源茉莉酸甲酯

Abstract: The effects of exogenous methyl jasmonate (MeJA) on the resistance levels to quinclorac and endogenous hormone levels were examined using the resistant and sensitive biotypes of barnyardgrass with the same genetic background. The results showed that exogenous MeJA could significantly increase the resistance of resistant plants to quinclorac, but did not affect the resistance of sensitive plants. There were significant differences in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA) among the tested materials. Quinclorac treatment could significantly increase the contents of ABA, SA, and JA in barnyardgrass, with stronger increase of ABA and JA contents in the sensitive plants. Exogenous MeJA could significantly enhance the induction of ABA and JA contents by quinclorac, especially in the sensitive biotypes. These results showed that rapid hormone variations in sensitive plants inhibited their response to herbicide, while gentle hormone variations endowed resistant plants with a strong adapta-bility to herbicide, which closely related to the enhanced resistance by MeJA. ABA and JA hormone signals might play an important role in the resistance of barnyardgrass to quinclorac stress.

Key words: barnyardgrass, quinclorac, herbicide resistance, phytohormone, exogenous MeJA

曹晶晶, 彭琼, 杨霞, 杨倩, 柏连阳, 李永丰, 张自常, 谷涛. 外源茉莉酸甲酯诱导抗性与敏感稗草对二氯喹啉酸抗性的差异及机理[J]. 应用生态学报, 2020, 31(7): 2293-2298.

CAO Jing-jing, PENG Qiong, YANG Xia, YANG Qian, BAI Lian-yang, LI Yong-feng, ZHANG Zi-chang, GU Tao. Differences in exogenous methyl jasmonate-induced quinclorac resistance between resistant and sensitive barnyardgrass and the underlying mechanism[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2293-2298.

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外源茉莉酸甲酯诱导抗性与敏感稗草对二氯喹啉酸抗性的差异及机理

Differences in exogenous methyl jasmonate-induced quinclorac resistance between resistant and sensitive barnyardgrass and the underlying mechanism

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