江苏省稻田马唐对3种乙酰辅酶A羧化酶抑制剂的抗性和靶标基因突变

doi:10.13287/j.1001-9332.202507.015

摘要/Abstract

摘要： 为明确江苏省稻田马唐对乙酰辅酶A羧化酶(ACCase)抑制剂类除草剂的抗性及靶标基因突变,利用区分剂量法测定了采自江苏省马唐发生严重稻区的59个田间种群对噁唑酰草胺、氰氟草酯、精噁唑禾草灵3种ACCase抑制剂类除草剂的抗性,并对ACCase基因突变进行分析。结果表明: 在噁唑酰草胺90 g·hm^-2的区分剂量下,2个马唐种群被标记为高抗性种群,8个种群被标记为抗性种群,3个种群被标记为发展中抗性种群;在氰氟草酯105 g·hm^-2的区分剂量下,8个马唐种群被标记为高抗性种群,14个种群被标记为抗性种群,8个种群被标记为发展中抗性种群;在精噁唑禾草灵20.7 g·hm^-2的区分剂量下,2个马唐种群被标记为高抗性种群,7个种群被标记为抗性种群,8个种群被标记为发展中抗性种群。对30个抗性马唐种群的靶标基因ACCase进行测序发现,18个种群发生了3种基因突变类型,分别是ACCase基因2027位点色氨酸(TGG)突变为半胱氨酸(TGC/TGT)、2027位点色氨酸(TGG)突变为丝氨酸(TCG)、2041位点异亮氨酸(ATT)突变为天冬酰胺(AAT)。综上,江苏省北部稻区马唐对ACCase抑制剂类除草剂的抗性发生较为严重,ACCase基因突变是导致其抗性的主要原因。

关键词: 马唐, 乙酰辅酶A羧化酶, 抗性监测, 基因突变, 水稻田

Abstract: To determine the acetyl-CoA carboxylase (ACCase) inhibitors resistance and diversity of the target ACCase gene mutations in Digitaria spp., we assessed the metamifop, cyhalofop-butyl, and fenoxaprop-P-ethyl resistance of 59 populations collected from rice fields in Jiangsu Province that were highly infested by Digitaria spp. by the discriminating dose assays, and analyzed the ACCase gene mutations. The results showed that there were 2, 8, and 3 Digitaria spp. populations marked as highly resistant, resistant, and developing resistant populations at the discriminating dose (90 g·hm^-2) of metamifop, respectively. There were 8, 14, and 8 populations identified as highly resistant, resistant, and developing resistant populations at the discriminating dose (105 g·hm^-2) of cyhalofop-butyl, respectively. There were 2, 7, and 8 populations confirmed as highly resistant, resistant, and deve-loping resistant populations at the discriminating dose (20.7 g·hm^-2) of fenoxaprop-P-ethyl, respectively. Target ACCase gene sequencing in 30 resistant Digitaria spp. populations revealed that there were three ACCase resistance mutations in 18 populations, including Trp (TGG)-2027-Cys (TGC/TGT), Trp (TGG)-2027-Ser (TCG), and Ile (ATT)-2041-Asn (AAT). In conclusion, the resistance of Digitaria spp. in northern region of Jiangsu Province to ACCase inhibitors herbicides was relatively severe, with ACCase gene mutations as the main cause of its resistance.

Key words: Digitaria spp., acetyl-CoA carboxylase, resistance detection, gene mutation, rice field

杨倩, 卫甜, 朱锦磊, 刘怀阿, 吕敏. 江苏省稻田马唐对3种乙酰辅酶A羧化酶抑制剂的抗性和靶标基因突变[J]. 应用生态学报, 2025, 36(7): 2083-2091.

YANG Qian, WEI Tian, ZHU Jinlei, LIU Huai’a, LYU Min. Resistance of three acetyl-CoA carboxylase (ACCase) inhibitors and the target gene mutations in Digitaria spp. from rice fields of Jiangsu Province, China[J]. Chinese Journal of Applied Ecology, 2025, 36(7): 2083-2091.

参考文献

[1] 杜浩, 刘学敏, 周劲松, 等. 马唐的综合防控研究进展. 安徽农业科学, 2022, 50(8): 26-28
[2] 郭文磊, 于超杰, 田兴山. 马唐密度对旱直播稻生长和产量性状的影响及其防治经济阈值. 西北农林科技大学学报: 自然科学版, 2023, 51(5): 101-109
[3] 戴魏真, 陆灯清, 许锦程, 等. 直播稻田马唐对噁唑酰草胺的抗性机制研究. 东北农业大学学报, 2023, 54(10): 1-10
[4] 郭文磊, 张泰劼. 张纯, 等. 马唐种子萌发及幼苗建成对不同环境因子的响应. 植物保护, 2022, 8(2): 85-93
[5] Yang Q, Zhu JL, Yang X, et al. Ile-1781-Leu target mutation and non-target-site mechanism confer resistance to acetyl-CoA carboxylase-inhibiting herbicides in Digitaria ciliaris var. chrysoblephara. Journal of Agricultural and Food Chemistry, 2023, 71: 7988-7995
[6] Kaundun SS. Resistance to acetyl-CoA carboxylase-inhibiting herbicides. Pest Management Science, 2014, 70: 1405-1417
[7] Heap I. The international survey of herbicide resistant weeds[EB/OL].[2025-11-28]. https://www.weedscience.org
[8] 蒋易凡, 陈国奇, 董立尧. 稻田马唐对稻田常用茎叶处理除草剂的抗性水平研究. 杂草学报, 2017, 35(2): 67-72
[9] 谷承文, 张立磊, 范玉洁, 等. 安徽稻区马唐对噁唑酰草胺的抗性测定及防治药剂筛选. 安徽科技学院学报, 2023, 37(4): 42-47
[10] Yang Q, Deng W, Liu LW, et al. Resistance patterns and molecular basis to ACCase-inhibiting herbicides. Weed Science, 2024, 72: 352-359
[11] Powles SB, Yu Q. Evolution in action: Plants resistant to herbicides. Annual Review of Plant Biology, 2010, 61: 317-347
[12] Gaines TA, Duke SO, Morran S, et al. Mechanisms of evolved herbicide resistance. Journal of Biological Che-mistry, 2020, 295: 10307-10330
[13] Zhang Y, Chen LP, Song W, et al. Diverse mechanisms associated with cyhalofop-butyl resistance in Chinese sprangletop (Leptochloa chinensis (L.) Nees): Characterization of target-site mutations and metabolic resis-tance-related genes in two resistant populations. Frontiers in Plant Science, 2022, 13: 990085
[14] Deng W, Li Y, Yao S, et al. Current status of cyhalofop-butyl and metamifop resistance and diversity of the ACCase gene mutations in Chinese sprangletop (Leptochloa chinensis) from China. Pesticide Biochemistry and Physiology, 2023, 197: 105648
[15] Deng W, Li Y, Yao S, et al. ACCase gene mutations and P450-mediated metabolism contribute to cyhalofop-butyl resistance in Eleusine indica biotypes from direct-seeding paddy fields. Pesticide Biochemistry and Physio-logy, 2023, 194: 105530
[16] Basak S, McElroy JS, Brown AM, et al. Plastidic ACCase Ile-1781-Leu is present in pinoxaden-resistant southern crabgrass (Digitaria ciliaris). Weed Science, 2020, 68: 41-50
[17] Iwakami S, Kamidate Y, Yamaguchi T, et al. CYP81A P450s are involved in concomitant cross-resistance to acetolactate synthase and acetyl-CoA carboxylase herbicides in Echinochloa phyllopogon. New Phytologist, 2019, 221: 2112-2122
[18] Cummins I, Wortley DJ, Sabbadin F, et al. Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110: 5812-5817
[19] Deng W, Yang MT, Li Y, et al. Enhanced metabolism confers a high level of cyhalofop-butyl resistance in a Chinese sprangletop (Leptochloa chinensis (L.) Nees) population. Pest Management Science, 2021, 77: 2576-2583
[20] Han HP, Yu Q, Beffa R, et al. Cytochrome P450 CYP81A10v7 in Lolium rigidum confers metabolic resistance to herbicides across at least five modes of action. Plant Journal, 2021, 105: 79-92
[21] Chen W, Bai DY, Liao YX, et al. PfGSTF2 endows resistance to quizalofop-p-ethyl in Polypogon fugax by GSH conjugation. Plant Biotechnology Journal, 2025, 23: 216-231
[22] Moss SR, Clarke JH, Blair AM, et al. The occurrence of herbicide-resistant grass-weeds in the United Kingdom and a new system for designating resistance in screening assays. Crop Protection Conference on Weeds, Brighton, UK, 1999: 179-184
[23] 邓维. 抗苯磺隆播娘蒿抗性机理及抗性突变对乙酰乳酸合成酶功能影响. 博士论文. 北京: 中国农业大学, 2017
[24] Bagavathiannan MV, Norsworthy JK, Smith KL, et al. Modeling the simultaneous evolution of resistance to ALS-and ACCase-inhibiting herbicides in barnyardgrass (Echinochloa crus-galli) in Clearfield^© Rice. Weed Technology, 2014, 28: 89-103
[25] 王红春, 徐蓬, 孙钰晨, 等. 江苏省稻田杂草的发生现状与防控建议. 杂草学报, 2019, 37(4): 1-5
[26] 袁国徽, 田志慧, 高原, 等. 上海市水稻田千金子对3种乙酰辅酶A羧化酶抑制剂的抗性现状及酶突变机制. 农药学学报, 2022, 24(3): 492-500
[27] Délye C, Jasieniuk M, Le Corre V. Deciphering the evolution of herbicide resistance in weeds. Trends in Gene-tics, 2013, 29: 649-658
[28] Cao JJ, Tao Y, Zhang ZC, et al. Mechanism of metamifop resistance in Digitaria ciliaris var. chrysoblephara from Jiangsu, China. Frontiers in Plant Science, 2023, 14: 1133798
[29] Délye C. Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: A major challenge for weed science in the forthcoming decade. Pest Management Science, 2013, 69: 176-187
[30] Liu XY, Hou ZL, Zhang YY, et al. Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop-P-methyl resistance in Digitaria sanguinalis. Pest Management Science, 2023, 79: 3950-3958
[31] 李淑顺, 强胜, 焦骏森. 轻型栽培技术对稻田潜杂草群落多样性的影响. 应用生态学报, 2009, 20(10): 2437-2445
[32] 张丹, 闵庆文, 成升魁, 等. 不同稻作方式对稻田杂草群落的影响. 应用生态学报, 2010, 21(6): 1603-1608
[33] 戴晓琴, 欧阳竹, 李运生. 耕作措施和施肥方式对麦田杂草密度和生物量的影响. 生态学杂志, 2011, 30(2): 234-240