氮添加和干旱条件下暖温带-亚热带针阔混交林土壤胞外酶活性及化学计量特征

doi:10.13287/j.1001-9332.202608.048

应用生态学报 ›› 2026, Vol. 37 ›› Issue (8): 2491-2498.doi: 10.13287/j.1001-9332.202608.048

氮添加和干旱条件下暖温带-亚热带针阔混交林土壤胞外酶活性及化学计量特征

林雨蝶^1,2,3, 陈志杰^1,2,3, 王世喆^1,2,3, 许敏慧^1,2,3, 江子怡^1,2,3, 施秀珍^1,2,3, 苏磊⁴, 王建青^1,2,3*

¹福建师范大学地理研究所, 福州 350117;
²福建师范大学地理科学学院/碳中和未来技术学院, 福州 350117;
³福建师范大学湿润亚热带生态-地理过程教育部重点实验室, 福州 350117;
⁴河南大学生命科学学院全球变化生态学国际联合实验室, 河南开封 475004

收稿日期:2026-05-14 修回日期:2026-07-11 出版日期:2026-08-18 发布日期:2027-02-18
通讯作者: ^*E-mail: jianqingwang@fjnu.edu.cn
作者简介:林雨蝶, 女, 2003年生, 硕士研究生。主要从事森林生态学研究。E-mail: 3216312490@qq.com
基金资助:
国家自然科学基金项目(32271679)、福建省自然科学基金项目(2024J09029)和福建省中青年教师教育科研项目(JZ230007)

Soil extracellular enzyme activities and stoichiometric characteristics in a warm-temperate-subtropical mixed coniferous and broad-leaved forest under nitrogen addition and drought conditions

LIN Yudie^1,2,3, CHEN Zhijie^1,2,3, WANG Shizhe^1,2,3, XU Minhui^1,2,3, JIANG Ziyi^1,2,3, SHI Xiuzhen^1,2,3, SU Lei⁴, WANG Jianqing^1,2,3*

¹Institute of Geography, Fujian Normal University, Fuzhou 350117, China;
²School of Geographical Sciences/School of Carbon Neutrality Future Technology, Fujian Normal University, Fuzhou 350117, China;
³Key Laboratory for Humid Subtropical Eco-Geographical Processes of Ministry of Education, Fujian Normal University, Fuzhou 350117, China;
⁴International Joint Research Laboratory for Global Change Ecology, School of Life Sciences, Henan University, Kaifeng 475004, Henan, China

Received:2026-05-14 Revised:2026-07-11 Online:2026-08-18 Published:2027-02-18

摘要/Abstract

摘要： 本研究以暖温带-亚热带气候过渡区针阔混交林土壤为对象,依托持续9年的野外控制试验,以自然降水且不施氮为对照,设置氮添加(50 kg N·hm^-2·a^-1)、干旱(降水减少50%)及氮添加+干旱处理,探究不同处理下土壤碳、氮、磷相关胞外酶活性及其化学计量特征。结果表明:与对照相比,氮添加使土壤β-葡萄糖苷酶(βG)活性、土壤酶化学计量碳氮比(E_C∶N)和土壤酶化学计量碳磷比(E_C∶P)分别显著提高113.1%、158.5%和126.8%;干旱使土壤酸性磷酸酶(AP)活性显著降低69.3%,而E_C∶N和E_C∶P分别显著提高125.9%和133.0%;氮添加+干旱使土壤βG活性和E_C∶P分别显著提高106.5%和129.8%。此外,氮添加+干旱处理显著提高了土壤AP和β-N-乙酰氨基葡萄糖苷酶(NAG)活性,但干旱处理削弱了氮添加对土壤βG活性、E_C∶N和E_C:P的促进作用。相关性分析表明,土壤纤维素水解酶(CBH)活性与土壤含水量呈显著正相关,土壤βG活性与可溶性有机碳、硝态氮和铵态氮呈显著正相关,土壤AP活性与铵态氮呈显著正相关,土壤NAG活性与可溶性有机碳和铵态氮呈显著正相关。综上,氮添加和干旱交互影响暖温带-亚热带针阔混交林土壤胞外酶活性及其化学计量特征,表明干旱潜在改变氮添加对土壤微生物养分获取酶投资策略的影响。

关键词: 氮沉降, 干旱, 土壤胞外酶, 化学计量特征, 森林土壤

Abstract: We focused on soils from coniferous-broadleaf mixed forests in the transition zone between warm tempe-rate and subtropical climates. Based on a 9-year field manipulative experiment with factorial treatments of nitrogen addition (50 kg N·hm^-2·a^-1) and drought (50% precipitation reduction). Soil extracellular enzyme activities involved in carbon, nitrogen, and phosphorus cycling and their stoichiometric characteristics were determined. Results showed that nitrogen addition significantly increased the activity of soil β-glucosidase (βG), soil enzyme stoichiometric carbon-nitrogen ratio (E_C:N) and soil enzyme stoichiometric carbon-phosphorus ratio (E_C:P) by 113.1%, 158.5%, and 126.8%, respectively. Drought significantly reduced the activity of soil acid phosphatase (AP) by 69.3%, while E_C:N and E_C:P increased significantly by 125.9% and 133.0%. Nitrogen addition combined with drought treatment significantly increased soil βG activity and E_C:P by 106.5% and 129.8%, respectively. Additionally, nitrogen addition combined with drought treatment significantly increased soil AP and β-N-acetylglucosaminidase (NAG) activities, whereas drought attenuated the positive effects of nitrogen addition on soil βG, E_C:N, and E_C:P. Soil cellulose hydrolase (CBH) activity was significantly positively correlated with soil water content. Soil βG activity was significantly positively correlated with dissolved organic carbon, nitrate, and ammonium. Soil AP activity was significantly positively correlated with ammonium. Soil NAG activity was significantly positively correlated with dissolved organic carbon and ammonium. In conclusion, nitrogen addition and drought interactively affected soil extracellular enzyme activities and their stoichiometric characteristics in a warm-temperate to subtropical mixed coniferous and broad-leaved forest, indicating that drought may alter the influence of nitrogen addition on soil microbial enzymatic strategies for nutrient acquisition.

Key words: nitrogen deposition, drought, soil extracellular enzyme, stoichiometric characteristics, forest soil

林雨蝶, 陈志杰, 王世喆, 许敏慧, 江子怡, 施秀珍, 苏磊, 王建青. 氮添加和干旱条件下暖温带-亚热带针阔混交林土壤胞外酶活性及化学计量特征[J]. 应用生态学报, 2026, 37(8): 2491-2498.

LIN Yudie, CHEN Zhijie, WANG Shizhe, XU Minhui, JIANG Ziyi, SHI Xiuzhen, SU Lei, WANG Jianqing. Soil extracellular enzyme activities and stoichiometric characteristics in a warm-temperate-subtropical mixed coniferous and broad-leaved forest under nitrogen addition and drought conditions[J]. Chinese Journal of Applied Ecology, 2026, 37(8): 2491-2498.

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氮添加和干旱条件下暖温带-亚热带针阔混交林土壤胞外酶活性及化学计量特征

Soil extracellular enzyme activities and stoichiometric characteristics in a warm-temperate-subtropical mixed coniferous and broad-leaved forest under nitrogen addition and drought conditions

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