Frankia菌的遗传多样性的RAPD研究

应用生态学报 ›› 1998, Vol. 9 ›› Issue (1): 59-63.

Frankia菌的遗传多样性的RAPD研究

彭源东, 张忠泽

中国科学院沈阳应用生态研究所, 沈阳 110015

收稿日期:1997-09-24 修回日期:1997-12-04 出版日期:1998-01-25 发布日期:1998-01-25
基金资助:
国家自然科学基金(39470005);中国科学院“九五”重大B项目

RAPD study on genetic diversity of Frankia

Peng Yuandong, Zhang Zhongze

Instituteof Applied Ecology, Academia Sinica, Shenyang 110015

Received:1997-09-24 Revised:1997-12-04 Online:1998-01-25 Published:1998-01-25

摘要/Abstract

摘要： 选用20个随机引物,对分自2个分类接种群的8株Frandia纯培养的总DNA进行随机扩增,其中引物OPW15和OPW16能扩增得到较为稳定的R_APD图谱.扩增产物分子量大都分布在0.5～4Kb之间.从稳定的R_APD扩增图谱看,Frankia菌间存在有丰富的遗传多样性；在选定适当引物情况下,能依据共同带将Frankia菌化归为同一分类接种群.

关键词: Frankia, RAPD, 多样性, 抗寒性, 解剖结构, 低温半致死温度

Abstract: Eight Frankia strains from 2 taxonomic inoculation groups were analysed for their genetic diversity with Random Amplified Polymorphic DNA(RAPD) technique. Twenty random primerswere used, and the stable RAPDpatterns could be detected with primer OPW15 and OPW16.The molecular weight of amplification products was between 0.5～4Kb, indicating a great genetic diversity withim Frankia strains. Therefore, with proper primer in RAPDanalysis, different Frankia strains could be allocated to the same inoculation group, based on commonbands.

Key words: Frankia, Random Amplified Polymorphic DNA(RAPD), Diversity, cold resistance, anatomical structure, semilethal low temperature (LT₅₀)

彭源东, 张忠泽. Frankia菌的遗传多样性的RAPD研究[J]. 应用生态学报, 1998, 9(1): 59-63.

Peng Yuandong, Zhang Zhongze . RAPD study on genetic diversity of Frankia[J]. Chinese Journal of Applied Ecology, 1998, 9(1): 59-63.

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