杭州西湖水体中异养细菌生长的限制因子

应用生态学报 ›› 2003, Vol. ›› Issue (8): 1343-1346.

杭州西湖水体中异养细菌生长的限制因子

吴根福¹, 杨志坚¹, 周雪平¹, 吴洁²

1. 浙江大学, 杭州 310028;
2. 杭州市环境保护监测站, 杭州 310007

收稿日期:2001-06-20 修回日期:2001-10-08
通讯作者: 吴根福,男,1965年生,硕士,副教授,主要从事环境微生物方面的研究工作,发表论文近30篇.E-mail:wu-genfu@zju.edu.cn.
基金资助:
浙江省自然科学基金(9515106);中日合作基金资助项目

Factors restricting growth of heterotrophic bacteria in the water body of West Lake, Hangzhou

WU Genfu¹, YANG Zhijian¹, ZHOU Xueping¹, WU Jie²

1. Zhejiang University, Hangzhou 310028, China;
2. Hangzhou Manicipal Environmental Monitoring Center, Hangzhou 310007, China

Received:2001-06-20 Revised:2001-10-08

摘要/Abstract

摘要： 通过添加不同营养盐类的纯种和自然培养试验,对异养细菌生长的限制因子进行了研究.结果表明,生物可利用的有机碳是主要的限制性营养因子,而氮源和磷源的影响相对较小;湖水的高pH、丰富的藻类和浮游动物生物量也制约了异养细菌的生长.此外还发现,在自然水体中添加0.5%葡萄糖后,一些自生固氮细菌得到富集;添加C+N+P后,有大量霉菌生长,添加0.01%牛肉膏后,假单胞菌属(Pseudomonas)细菌由原来的30%提高到57%;湖水进行实验室培养时细菌的最大生长量可达10⁵个·ml^-1.

关键词: 杭州, 西湖, 水体, 异养细菌, 限制因子

Abstract: Restricting factors of bacteria growth were studied by pure culture and natural culture test. The results showed that organic carbon source available for bacteria was more important than (NH₄)₂SO₄ and KH₂PO₄, while higher pH, and rich biomass of phytoplanktons and zooplanktons in the water restrained the growth of heterotrophic bacteria. Under natural culture experiment, Azotobacter increased after 0.5% glucose was added, and a lot of mildew grew after adding0.5% glucose with 0.1%(NH₄)₂SO₄ and 0.1% KH₂PO₄, while Pseudomonas enriched 30～57% after adding0.01% beef extract. It was also showed that bacteria growth potentiality in natural water could reach to 10⁵cfu·ml^-1.

Key words: Hangzhou, West Lake, Water body, Heterotrophic bacteria, Restricting factors

中图分类号:

Q938.8

吴根福, 杨志坚, 周雪平, 吴洁. 杭州西湖水体中异养细菌生长的限制因子[J]. 应用生态学报, 2003, (8): 1343-1346.

WU Genfu, YANG Zhijian, ZHOU Xueping, WU Jie. Factors restricting growth of heterotrophic bacteria in the water body of West Lake, Hangzhou[J]. Chinese Journal of Applied Ecology, 2003, (8): 1343-1346.

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