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高铜、高锌猪粪对蚯蚓的急性毒性效应研究

贾秀英1 罗安程2 李喜梅1   

  1. 1杭州师范学院生命科学学院,杭州 310036;2浙江大学环境与资源学院,杭州 310029
  • 收稿日期:2004-07-02 修回日期:2004-11-26 出版日期:2005-08-18

Acute toxicological effects of excessive Cu and Zn-containing in pig manure on earthworm

JIA Xiuying 1,LUO Ancheng 2,LI Ximei 1   

  1. 1School of Life Science,Hangzhou Normal College,Hangzhou 310036,Chin
    a;2Department of Environmental and Resources Science,Zhejiang University, H
    angzhou 310029,China

  • Received:2004-07-02 Revised:2004-11-26 Online:2005-08-18

摘要: 测定了高Cu、高Zn猪粪条件下Cu、Zn单一与复合污染对蚯蚓的急性致死及亚致死效应.结果表明,Cu、Zn浓度与蚯蚓死亡率显著正相关(a=0.05, rCu=0.99, rZn=0.99),与体重增长率显著负相关(a =0.05,rCu=-0.99,rZn=-0.96).蚯蚓个体对Cu、Zn的耐受程度不同,其毒性阈值(引起蚯蚓个体死亡浓度)分别为:Cu 250 mg·kg-1、Zn 400 mg·kg-1.LD50分别为:Cu 646.68 mg·kg-1、Zn 947.38 mg·kg-1.复合污染情况下,Cu浓度为250、500 mg·kg-1时,Cu、Zn复合污染表现为协同效应;Cu浓度为750 mg·kg-1时,Cu、Zn复合污染表现为拮抗效应.可见,猪粪中Cu、Zn复合污染的毒性效应与各组浓度组合密切相关.

关键词: 白三叶, 刈割, 能量分配, 构型

Abstract: Cu and Zn are popularly used as additives in animal feed in China,which could result in their excessive accumulation in manure to a toxic level,and thus,possibly threaten the environment when the manure is applied to farmland.In this paper,the acute and sub?acute lethal effects of pig manure with excessive Cu and Zn on earthworm were studied,and the results indicated that the concentration of Cu and Zn in pig manure had a significantly positive correlation with the mortality of earthworm,while a significantly negative correlation with earthworm growth rate.The individuals of earthworm had different tolerance to excessive Cu and Zn,with the threshold values causing death being 250 mg·kg-1 and 400 mg·kg-1 for Cu and Zn,respectively.The LD50 was 646.68 mg·kg-1 for Cu,and 947.38 mg·kg-1 for Zn.A strong synergistic effect was observed under the combined pollution of 250 mg·kg-1 for Cu and 500 mg·kg-1 for Zn,whereas antagonistic effect happened when 750 mg·kg-1 for Cu was supplied, suggesting that the joint toxic effects of Cu and Zn on earthworm were closely correlated to their concentrations in pig manure.

Key words: Trifolium repens, Cutting, Energy allocation, Architecture