胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析

应用生态学报

胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析

韩平¹;郑立¹;崔志松¹;郭秀春^1，2;田黎¹

¹国家海洋局第一海洋研究所海洋生态中心，山东青岛 266061;²中国海洋大学化学化工学院，山东青岛 266100

收稿日期:2008-10-08 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20

Isolation, identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil.

HAN Ping¹;ZHENG Li¹;CUI Zhi-song¹;GUO Xiu-chun^1,2;TIAN Li¹

¹Research Centre for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, Shandong, China;²College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, Shandong, China

Received:2008-10-08 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20

摘要/Abstract

摘要： 基于传统的实验方法，对胜利油田滩涂区土壤中石油降解菌进行了筛选和鉴定，并利用PCR-DGGE (变性梯度凝胶电泳)技术分析了胜利油田滩涂区的菌群多样性.结果表明：由研究区土壤中筛选出13株石油降解菌，其中，6株石油降解菌的石油降解率>90%，能降解大部分C₁₂～C₂₆的石油烷烃，系统发育学鉴定为Alcanivorax、Halomonas和Marinobacter，均属于γ-proteobacteria分支;胜利油田滩涂区未培养优势菌有Sulfurovum、Gillisia和Arcobacter;该区优势菌群中γ-proteobacteria所占比重较大，其次为α-proteobactiria、ε-proteobactiria、Actinobacteria 和Flavobacteria.

关键词: 气候变暖, 被动式夜间增温, 土壤pH值, 土壤速效养分, 根系, 冬小麦

Abstract: The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE (denaturing gradient gel electrophoresis). A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12〖KG-*2〗-〖KG-*7〗C26 petroleum hydrocarbon, with a degradation rate of >90%. These petroleum degraders were phylogeneticly identified as the members of Halomonas, Alcanivorax, and Marinobacter, which were all belonged to γ-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, Gillisia and Arcobacter. Among the predominant bacteria, γ-proteobacteria accounted for a larger proportion, followed by α-proteobactiria, ε-proteobactiria, Actinobacteria, and Flavobacteria.

Key words: climate warming, passive nighttime warming (PNW), soil pH, soil available nutrient, root system, winter wheat.

韩平¹;郑立¹;崔志松¹;郭秀春^1，2;田黎¹. 胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析[J]. 应用生态学报.

HAN Ping¹;ZHENG Li¹;CUI Zhi-song¹;GUO Xiu-chun^1,2;TIAN Li¹. Isolation, identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil.[J]. Chinese Journal of Applied Ecology.

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