高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响

应用生态学报 ›› 2012, Vol. 23 ›› Issue (11): 3016-3020.

高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响

张祥,王桂霞,顾超,韩勇,许映飞,陈源,陈德华^**

(扬州大学江苏省作物遗传生理重点实验室/农业部长江中下游作物生理生态与栽培重点开放实验室, 江苏扬州 225009)

出版日期:2012-11-18 发布日期:2012-11-18

Effects of high temperature and humidity on leaf Bt protein expression of transgenic Bt cotton.

ZHANG Xiang, WANG Gui-xia, GU Chao, HAN Yong, XU Ying-fei, CHEN Yuan, CHEN De-hua

(Jiangsu Province Key Laboratory of Crop Gentics and Physiology/Ministry of Agriculture Key Laboratory of Crop Physiology, Ecology and Cultivation in Middle and Lower Reaches of Yangtze River, Yangzhou University, Yangzhou 225009, Jiangsu, China)

Online:2012-11-18 Published:2012-11-18

摘要/Abstract

摘要： 以来源于美国的转Bt基因棉DP410B（常规种）和岱杂1号（杂交种）以及来源于中国的转Bt基因棉泗抗1号（常规种）和泗抗3号（杂交种）为材料，研究高温(37 ℃)条件下，大气湿度(50%、70%、90%)变化对Bt蛋白表达的影响.结果表明：高温条件下，盛蕾期，温湿度对4个供试品种Bt蛋白表达均无显著影响；盛花期，与对照(温度25~30 ℃，湿度60%～70%)相比，常规种在50%湿度时叶片Bt蛋白含量显著降低2.6%～3.0%；盛铃期，DP410B、泗抗1号和泗抗3号的Bt蛋白含量在50%湿度处理下比对照显著降低3.3%～5.8%.4个转Bt基因棉品种中，DP410B和岱杂1号的Bt蛋白含量最高，而泗抗1号最低.

Abstract: Different origins Bt cotton cultivars, including DP410B (conventional cultivar) and Daiza No.1 (hybridized cultivar) from US and Sikang No.1 (conventional cultivar) and Sikang No.3 (hybridized cultivar) from China, were taken as the test materials to investigate the effects of high temperature (37 ℃) and different humidity (50%, 70%, and 90%) on the leaf Bt protein expression of Bt cotton. At high temperature, temperature and humidity had no significant effects on the leaf Bt protein expression of the cultivars at peak squaring stage. At peak flowering stage, as compared with the control (25-30 ℃ and 60%-70% humidity), 37 ℃ and 50% humidity decreased the leaf Bt protein content of conventional cultivars significantly by 2.6%-3.0%. At peak bolling stage, compared with the control, 37 ℃ and 50% humidity decreased the leaf Bt protein content of DP410B, Sikang No.1, and Sikang No.3 significantly by 3.3%-5.8%. Among the four cultivars, DP410B and Daiza No.1 had the highest leaf Bt protein content, while Sikang No.1 had the lowest one.

张祥,王桂霞,顾超,韩勇,许映飞,陈源,陈德华**. 高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响[J]. 应用生态学报, 2012, 23(11): 3016-3020.

ZHANG Xiang, WANG Gui-xia, GU Chao, HAN Yong, XU Ying-fei, CHEN Yuan, CHEN De-hua. Effects of high temperature and humidity on leaf Bt protein expression of transgenic Bt cotton.[J]. Chinese Journal of Applied Ecology, 2012, 23(11): 3016-3020.