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• 研究简报 • 上一篇    

外源抗坏血酸对镉胁迫下黑藻抗氧化系统的保护作用

许丙军;施国新;徐勤松;王学;赵娟;胡金朝   

  1. 南京师范大学生命科学学院,南京 210097
  • 收稿日期:2005-08-31 修回日期:2006-06-08 出版日期:2006-09-18 发布日期:2006-09-18

Protective effects of exogenous ascorbic acid on antioxidant system in Hydrilla verticillata under Cd2+ stress

XU Bingjun;SHI Guoxin;XU Qinsong;WANG Xue;ZHAO Juan;HU Jinzhao   

  1. College of Life Science, Nanjing Normal University, Nanjing 210097, China
  • Received:2005-08-31 Revised:2006-06-08 Online:2006-09-18 Published:2006-09-18

摘要: 研究了不同浓度抗坏血酸(ascorbic acid,AsA)对5 mg·L-1Cd2+胁迫下黑藻(Hydrilla verticillata)体内超氧阴离子(O2-)产生速率、过氧化氢(H2O2)含量、抗坏血酸和谷胱甘肽(GSH)含量,以及超氧化物歧化酶(SOD)、超氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的影响.结果表明,与单一Cd2+胁迫相比,随着外源AsA浓度的升高,黑藻体内活性氧生成速率逐步降低;抗氧化物质AsA先升后降,GSH缓慢升高;抗氧化酶APX和CAT活性先升后降,POD活性逐步下降,并接近正常状态,对SOD活性影响不大.可见AsA能够有效缓解Cd2+对黑藻的毒害,且60 mg·L-1浓度下的缓解效果最好.

关键词: 鄂南棕红壤, 农田生态系统, B平衡

Abstract: In this paper, Hydrilla verticillata was cultured in the hoagland solution containing 5 mg·L-1 Cd2+ and different concentration ascorbic acid (AsA) to investigate the protective action of AsA to aquatic plant suffered from heavy metal′s stress. The O2-generation rate, H2O2 content, antioxidants (AsA and GSH) contents, and antioxidant enzymes (SOD, POD, CAT and APX) activities were analyzed. The results showed that compared with single Cd2+ treatment, AsA addition lessened the reactive oxygen species (O2- and H2O2) generation rates, endogenous AsA content, and antioxidant enzyme activities. It was concluded that exogenous AsA could relieve the Cd2+ poison to H. verticillata, and its optimum concentration was 60 mg·L-1.

Key words: Brown-red soil of south Hubei Province, Agroecosystem, Boron balance