氨氧化微生物在粉葛替代薇甘菊过程中的作用

doi:10.13287/j.1001-9332.202506.006

应用生态学报 ›› 2025, Vol. 36 ›› Issue (6): 1849-1858.doi: 10.13287/j.1001-9332.202506.006

氨氧化微生物在粉葛替代薇甘菊过程中的作用

曾子茵¹, 余涵霞², 周启梦¹, 游俊杰¹, 李伟华^1*

¹华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州 510631;
²广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室, 广州 510640

收稿日期:2025-01-12 接受日期:2025-03-17 出版日期:2025-06-18 发布日期:2025-12-18
通讯作者: *E-mail: whli@scnu.edu.cn
作者简介:曾子茵, 女, 2003年生, 本科生。主要从事土壤微生物研究。E-mail: zzy20221479@163.com
基金资助:
国家自然科学基金面上项目(32172430)

The role of ammonia-oxidizing microorganisms in the replacement of Mikania micrantha by Pueraria lobata

ZENG Ziyin¹, YU Hanxia², ZHOU Qimeng¹, YOU Junjie¹, LI Weihua^1*

¹Guangdong Province Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China;
²Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs/Guangdong Province Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2025-01-12 Accepted:2025-03-17 Online:2025-06-18 Published:2025-12-18

摘要/Abstract

摘要： 本文对氨氧化微生物在粉葛替代控制入侵植物薇甘菊过程中的作用进行了研究,分析了粉葛替代对薇甘菊再生、植物组织氮素吸收、土壤氮素转化及氨氧化微生物群落的影响。结果表明: 在粉葛替代控制1年后,土壤总氮、铵态氮和硝态氮分别显著增加了109.4%、78.1%和20.3%,净硝化速率显著提高了213.0%;氨氧化细菌AOB-amoA基因拷贝数显著增加,并与土壤总氮、硝态氮呈显著正相关。随机森林模型分析表明,完全氨氧化菌comammox clade A.2和氨氧化细菌AOB共同主导了净硝化作用,土壤净硝化速率、AOB-amoA基因拷贝数和植物组织总氮含量是粉葛相对盖度增加的主要影响因子。氨氧化微生物活性的增强提高了土壤氮素转化效率,使粉葛在氮素吸收与积累方面优于薇甘菊,有效抑制了薇甘菊的再生。

关键词: 薇甘菊, 替代控制, 粉葛, 氨氧化微生物, 硝化作用

Abstract: We investigated the role of ammonia-oxidizing microorganisms in the process of Pueraria lobata replacement for controlling the invasive plant, Mikania micrantha. We examined the effects of P. lobata replacement on the regeneration of M. micrantha, plant nitrogen absorption, soil nitrogen transformation, and ammonia-oxidizing microbial communities. The results demonstrated that one year after P. lobata replacement, there were significant increases in soil total nitrogen (109.4%), ammonium (78.1%), and nitrate (20.3%), accompanied by a remarkable 213.0% elevation in the net nitrification rate. The ammonia-oxidizing bacteria AOB-amoA gene copy number was significantly increased, which was positively correlated with soil total nitrogen and nitrate nitrogen. Results of random forest model analysis showed that comammox clade A.2 and AOB jointly dominated the nitrification process. The soil net nitrification rate, AOB-amoA gene copy number, and plant tissue total nitrogen content were identified as the primary factors influencing the relative cover of P. lobata. The enhanced activity of ammonia-oxidizing microorganisms improved soil nitrogen conversion efficiency, giving P. lobata a competitive advantage over M. micrantha in nitrogen uptake and accumulation, thereby effectively inhibiting the regeneration of M. micrantha.

Key words: Mikania micrantha, replacement control, Pueraria lobata, ammonia-oxidizing microorganism, nitrification

曾子茵, 余涵霞, 周启梦, 游俊杰, 李伟华. 氨氧化微生物在粉葛替代薇甘菊过程中的作用[J]. 应用生态学报, 2025, 36(6): 1849-1858.

ZENG Ziyin, YU Hanxia, ZHOU Qimeng, YOU Junjie, LI Weihua. The role of ammonia-oxidizing microorganisms in the replacement of Mikania micrantha by Pueraria lobata[J]. Chinese Journal of Applied Ecology, 2025, 36(6): 1849-1858.

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氨氧化微生物在粉葛替代薇甘菊过程中的作用

The role of ammonia-oxidizing microorganisms in the replacement of Mikania micrantha by Pueraria lobata

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