微囊藻毒素-LR慢性暴露对菖蒲磷吸收及积累的影响

doi:10.13287/j.1001-9332.202510.033

摘要/Abstract

摘要： 为研究微囊藻毒素-LR(MC-LR)慢性暴露对菖蒲磷吸收及积累的影响,在MC-LR浓度为0(对照)、1、10、30 μg·L^-1的水培体系中,对菖蒲进行25 d的胁迫培养,测定不同处理菖蒲生长状况、根系活力、丙二醛(MDA)含量、2C型丝氨酸/苏氨酸蛋白磷酸酶(PP2C)活性以及菖蒲磷吸收特性和磷积累量的变化。结果表明:1 μg·L^-1 MC-LR暴露后,菖蒲根系生物量增加12.1%,根系活力增强33.6%,植株对磷的亲和力及吸收潜能上升,从而促进了菖蒲对磷的吸收和积累。10 μg·L^-1 MC-LR暴露后,菖蒲根系活力和MDA含量无显著变化,而根系PP2C活性下降25.4%,对菖蒲磷吸收和积累有一定程度的负面影响。30 μg·L^-1 MC-LR暴露后,菖蒲根系活力下降25.8%,MDA含量上升48.9%,PP2C活性降低48.5%,根系生物量减少11.2%,植株对磷的亲和力及吸收潜能下降,抑制了菖蒲对磷的吸收和积累,但磷优先向地上部转移,以满足茎叶对磷的需求。表明在试验设计的浓度范围内,较高浓度(≥10 μg·L^-1) MC-LR慢性暴露对菖蒲磷吸收和积累具有不同程度的抑制作用,会对菖蒲生长产生不利影响。

关键词: 微囊藻毒素, 菖蒲, 磷吸收, 根系活力

Abstract: To investigate the effects of chronic exposure to microcystin-LR (MC-LR) on phosphorus (P) uptake and accumulation in Acorus calamus, we examined the growth status, root vigor, malondialdehyde (MDA) content, type 2C serine/threonine protein phosphatase (PP2C) activity, as well as changes in phosphorus uptake characte-ristics and phosphorus accumulation in A. calamus under MC-LR concentrations of 0 (control), 1, 10, and 30 μg·L^-1 in a hydroponic system for 25 days. The results showed that after exposure to 1 μg·L^-1 MC-LR, root biomass of A. calamus increased by 12.1%, root vigor enhanced by 33.6%, and the plant’s affinity and uptake potential for phosphorus increased, thereby promoting phosphorus uptake and accumulation. After exposure to 10 μg·L^-1 MC-LR, root vigor and MDA content of A. calamus showed no significant change, while root PP2C activity decreased by 25.4%, with negative impact on phosphorus uptake and accumulation. After exposure to 30 μg·L^-1 MC-LR, root vigor of A. calamus decreased by 25.8%, MDA content increased by 48.9%, PP2C activity reduced by 48.5%, root biomass decreased by 11.2%, and the plant’s affinity and uptake potential for phosphorus decreased, inhibiting phosphorus uptake and accumulation. However, phosphorus preferentially transferred to the aboveground part to meet the demand of stems and leaves for phosphorus. Our results indicated that chronic exposure to higher concentrations (≥10 μg·L^-1) of MC-LR had varying degrees of inhibitory effects on phosphorus uptake and accumulation in A. calamus, which would adversely affect its growth.

Key words: microcystin, Acorus calamus, phosphorus uptake, root vigor

陈国元, 叶凡, 姜子涵, 李青松. 微囊藻毒素-LR慢性暴露对菖蒲磷吸收及积累的影响[J]. 应用生态学报, 2025, 36(10): 3187-3192.

CHEN Guoyuan, YE Fan, JIANG Zihan, LI Qingsong. Effects of chronic exposure to microcystin-LR on phosphorus uptake and accumulation of Acorus calamus.[J]. Chinese Journal of Applied Ecology, 2025, 36(10): 3187-3192.

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