施磷和接种丛枝菌根真菌对钒胁迫下玉米生长的影响

doi:10.13287/j.1001-9332.202412.030

应用生态学报 ›› 2024, Vol. 35 ›› Issue (12): 3444-3452.doi: 10.13287/j.1001-9332.202412.030

施磷和接种丛枝菌根真菌对钒胁迫下玉米生长的影响

裘浪^1,2, 王志刚³, 俞龙生⁴, 张云云¹, 余浩¹, 张延旭^5*

¹宿州学院环境与测绘工程学院, 安徽宿州 234000;
²广东省科学院生态环境与土壤研究所, 广州 510650;
³中国矿业大学(北京)地球科学与测绘工程学院, 北京 100083;
⁴广州市环境保护科学研究院有限公司, 广州 510620;
⁵中国矿业大学(北京)煤炭精细勘探与智能开发全国重点实验室, 北京 100083

收稿日期:2024-04-06 接受日期:2024-09-18 出版日期:2024-12-18 发布日期:2025-06-18
通讯作者: *E-mail: zhangyanxu911@163.com
作者简介:裘浪, 男, 1988年生, 博士,讲师。主要从事丛枝菌根真菌与土壤重金属修复研究。E-mail: lq88@ahszu.edu.cn
基金资助:
安徽省自然科学基金项目(2308085QD117)、安徽省教育厅高校科学研究项目(2022AH051385)和宿州学院博士科研启动基金项目(2023BSK060)

Effects of arbuscular mycorrhizal fungi inoculation and phosphorus application on maize growth under vanadium stress

QIU Lang^1,2, WANG Zhigang³, YU Longsheng⁴, ZHANG Yunyun¹, YU Hao¹, ZHANG Yanxu^5*

¹School of Environment and Surveying Engineering, Suzhou University, Suzhou 234000, Anhui, China;
²Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China;
³College of Geoscience and Surveying Engineering, China University of Mining and Technology-Beijing, Beijing 100083, China;
⁴Guangzhou Research Institute of Environmental Protection, Guangzhou 510620, China;
⁵State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology-Beijing, Beijing 100083, China

Received:2024-04-06 Accepted:2024-09-18 Online:2024-12-18 Published:2025-06-18

摘要/Abstract

摘要： 钒(V)与磷(P)具有类似的化学结构,而丛枝菌根真菌(AMF)对P元素具有高度的亲和性。为了解钒胁迫下接种AMF和施磷对玉米生长的影响,采用玉米盆栽试验,在钒处理(0、100、200和400 mg·kg^-1)下接种AMF和施P(0、20和50 mg·kg^-1),测定玉米生长、P与V吸收以及植株体内钒形态分布。结果表明: 与对照相比,钒胁迫(100、200和400 mg·kg^-1)显著降低了玉米根系AMF侵染率和生物量,其中200和400 mg·kg^-1 钒水平下的AMF侵染率分别显著降低14.9%～28.0%和29.5%～31.9%,生物量相应降低41.4%～42.2%和54.3%～55.3%;钒胁迫显著抑制玉米地上部对P的吸收,提高了地上部和根系V浓度和吸收量。接种AMF和施磷能够缓解钒胁迫对玉米生长的抑制作用,显著降低玉米V富集系数(23.3%～47.7%)和转运系数(13.1%～27.9%)以及玉米V吸收量,提高玉米地上部和根系生物量以及P吸收量。接种AMF与施磷对玉米根系AMF侵染率、地上部的生物量和P、V吸收量具有显著交互作用。分析玉米植株体内金属V形态发现,接种AMF可促进玉米将吸收的高毒性V还原为低毒性V。接种AMF主要通过促进玉米生长和P吸收、抑制V吸收来缓解钒胁迫,施磷则可进一步起到改善作用。

关键词: 钒, 丛枝菌根真菌, 磷吸收, 侵染率, 迁移转运

Abstract: Vanadium (V) and phosphorus (P) are similar in chemical structure. Arbuscular mycorrhizal fungi (AMF) have high affinity for P. To investigate the effects of AMF inoculation and P application on maize growth under V stress, we investigated the effects of AMF inoculation and P application (0, 20 and 50 mg·kg^-1 P₂O₅) on maize growth, P and V uptake, and V speciation in the soil amended with four levels of V addition (0, 100, 200, and 400 mg·kg^-1V). The results showed that V stress (100, 200 and 400 mg·kg^-1) significantly decreased root mycorrhizal colonization and plant biomass. The 200 and 400 mg·kg^-1 V stress treatments significantly decreased root mycorrhizal colonization by 14.9%-28.0% and 29.5%-31.9%, and reduced plant biomass by 41.4%-42.2% and 54.3%-55.3%, respectively. V stress significantly decreased P concentration and uptake of maize shoot and increased V concentration and uptake of maize shoot and root. AMF inoculation and P application could alleviate the inhibition effect of V on maize growth, which significantly decreased bioconcentration factor of V by 23.3%-47.7% and translocation factor of V by 13.1%-27.9%, as well as V concentration and uptake of maize shoot, but improved plant biomass and P uptake of maize shoot and root. The factor of AMF inoculation and P application had interaction on root mycorrhizal colonization and plant biomass, as well as P and V uptake of maize shoot. AMF inoculation promoted the reduction of V_V into V_IV in plant tissues. AMF inoculation could alleviate V stress by promoting maize growth and P uptake and inhibiting V translocation, with synergistic effects from P application.

Key words: vanadium, arbuscular mycorrhizal fungi, phosphorus uptake, mycorrhizal colonization rate, translocation

裘浪, 王志刚, 俞龙生, 张云云, 余浩, 张延旭. 施磷和接种丛枝菌根真菌对钒胁迫下玉米生长的影响[J]. 应用生态学报, 2024, 35(12): 3444-3452.

QIU Lang, WANG Zhigang, YU Longsheng, ZHANG Yunyun, YU Hao, ZHANG Yanxu. Effects of arbuscular mycorrhizal fungi inoculation and phosphorus application on maize growth under vanadium stress[J]. Chinese Journal of Applied Ecology, 2024, 35(12): 3444-3452.

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施磷和接种丛枝菌根真菌对钒胁迫下玉米生长的影响

Effects of arbuscular mycorrhizal fungi inoculation and phosphorus application on maize growth under vanadium stress

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