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应用生态学报 ›› 1999, Vol. 10 ›› Issue (6): 732-734.

• 研究论文 • 上一篇    下一篇

黑曲霉生产β-葡萄糖苷酶发酵条件的研究产

李平1, 宛晓春1, 陶文沂2, 丁霄霖2   

  1. 1. 安徽农业大学轻工业学院, 合肥230036;
    2. 无锡轻工大学, 无锡214036
  • 收稿日期:1999-02-01 修回日期:1999-05-25 出版日期:1999-11-25 发布日期:1999-11-25
  • 通讯作者: 李 平,女,1968年出生,工学硕士,讲师,主要从事食品与发酵工程学科方面的研究工作,发表论文多篇.E-mail:zhwzhang@mail.hf.ah.cn E-mail:zhwzhang@mail.hf.ah.cn
  • 基金资助:

    国家“八五”科技攻关项目(85-609-02-03).

Fermentation conditions of β-glucosidase production from Asp niger

Li Ping1, Wan Xiaochun1, Tao Wenyi2, Ding Xiaolin2   

  1. 1. Anhui Agricultural University, Hefei 230036;
    2. Wuxi Light Industry University, Wuxi 214036
  • Received:1999-02-01 Revised:1999-05-25 Online:1999-11-25 Published:1999-11-25

摘要: 经多项式回归分析,研究了不同浓度N源、C源、无机盐等对酶产量的影响,确定出最佳培养基配方为:麸皮4.9%,(NH4)2>SO40.4%,KH2PO40.29%,CaCl20.05%,MgSO4·7H2O0.04%,FeSO4·7H2O 5mg·L-1,ZnCl21.4mg·L-1,0.2%油酸钠。并对培养温度、时间、培养基初始pH、通气量、接种量、接种方式等培养条件进行优化,使黑曲霉生产β葡萄糖苷酶的产量由17U·ml-1增至21.3U·ml-1.

关键词: 多项式回归, 发酵条件, &beta, -葡萄糖苷酶酶活

Abstract: Through multinomial regression, the effects of different concentrations of carbon source, nitrogen source and inorganic salts on β glucosidase production were studied. The optimum components of mediums consisted of bran 4.9%, (NH4)2SO4 0.4%, KH2PO4 0.29%, CaCl2 0.05%, MgSO4·7H2O0.04%, FeSO4·7H2O 5mg·L-1, ZnCl 21.4mg·L-1, and sodium oleate 0.2%. The fermentation conditions (including temperature, time, pH, aeration, and inoculation rate and pattern) were also optimized. Under the optimized conditions, the activity of β-glucosidase produced from Asp. niger was increased from 17U·ml-1 to 21.3U·ml-1.

Key words: Multinomial regression, Fermentation condition, β- glucosidase activity