利用隆线溞趋光行为评价铬的生物毒性

应用生态学报 ›› 2005, Vol. 16 ›› Issue (1): 171-174.

利用隆线溞趋光行为评价铬的生物毒性

吴永贵^1,2, 黄建国¹, 袁玲¹

1. 华南农业大学资源环境学院, 广州, 510642;
2. 西南农业大学资源环境学院, 重庆, 400716

收稿日期:2003-11-18 修回日期:2004-02-15 出版日期:2005-01-15 发布日期:2005-01-15
通讯作者: 吴永贵,男,1972年生,博士,讲师,现为华南农业大学资源环境学院在站博士后,主要从事植物营养与环境生态方面的教学研究工作,发表论文多篇.
基金资助:
重庆市科委应用基础重点资助项目(2002-7301).

Phototactic behavior of Daphnia carinata as an indicator of chromium biotoxicity

WU Yonggui^1,2, HUANG Jianguo¹, YUAN Ling ¹

1. College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;
2. College of Resources and Environment, Southwest Agricultural University, Chongqing 400716, China

Received:2003-11-18 Revised:2004-02-15 Online:2005-01-15 Published:2005-01-15

摘要/Abstract

摘要： 报道了以隆线溞单克隆Dc2为生物监测器,利用其趋光行为变化评价铬生物毒性的方法.结果表明,隆线溞趋光行为抑制率能较好地反映水中铬的污染程度.在重铬酸钾标准毒物溶液中,溞趋光指数与Cr⁶⁺的浓度呈极显著负相关(R²=0.8089,P＜0.001),Cr⁶⁺浓度的检测下限为0.056 mg·L^-1,远低于LC₅₀和EC₅₀,平均精度达到5.6%,说明溞趋光指数法用于监测化学物质生物毒性灵敏度高、精确可靠.

关键词: 铬, 隆线溞, 趋光行为, 生物监测

Abstract: The study on the phototactic behavior of Daphnia carinata clone Dc42 as an indicator of chromium biotoxicity showed that the phototaxis inhibition ratio (Rpi) of D.carinata could better indicate the chromium status in water.There was a negative linear correlation between phototactic index (I_p) and Cr⁶⁺ concentration in standardized K₂Cr₂O₇ solutions (R²=0.8089,P<0.001).The detection limit of Cr⁶⁺ by I_p was 0.056 mg稬^-1,much lower than that of LC₅₀ or EC₅₀,and the mean precision index for detection reached up to 5.46%,which suggested the sensitivity,precision and reliability of this method in monitoring chemicals biotoxicity.

Key words: Chromium, Daphnia carinata, Phototactic behavior, Biomonitor

中图分类号:

Q948.3

吴永贵, 黄建国, 袁玲. 利用隆线溞趋光行为评价铬的生物毒性[J]. 应用生态学报, 2005, 16(1): 171-174.

WU Yonggui, HUANG Jianguo, YUAN Ling . Phototactic behavior of Daphnia carinata as an indicator of chromium biotoxicity [J]. Chinese Journal of Applied Ecology, 2005, 16(1): 171-174.

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