秸秆预处理对土壤微生物量及呼吸活性的影响

应用生态学报 ›› 2003, Vol. ›› Issue (12): 2225-2228.

秸秆预处理对土壤微生物量及呼吸活性的影响

李贵桐, 张宝贵, 李保国

中国农业大学资源与环境学院, 北京 100094

收稿日期:2001-03-29 修回日期:2001-07-02 出版日期:2003-12-15
通讯作者: 李贵桐,男,1968年生,博士,副教授.主要从事土壤生物化学、土壤生物多样性与生态、土壤养分循环等方面的研究,发表论文近20余篇.Tel:010-62892963,E-mail:lgtong@mail.cau.edu.cn.李贵桐,通讯联系人.
基金资助:
国家重点基础研究发展规划资助项目(G1999011709)

Effect of straw pretreatment on soil microbial biomass and respiration activity

LI Guitong, ZHANG Baogui, LI Baoguo

College of Resource and Environment, China Agricultural University, Beijing 100094, China

Received:2001-03-29 Revised:2001-07-02 Online:2003-12-15

摘要/Abstract

摘要： 冬小麦秸秆经8.0g·L^-1H2O₂(pH11.0)溶液、12.5g·L^-1NaOH溶液或H2SO4溶液浸泡8h并80℃烘干后,与无机N一起加入土壤,进行室内25℃恒温培养试验,在不同时间测定土壤微生物量C、N和CO₂释放速率.结果表明,培养前期,秸秆预处理使土壤微生物量C数量增加了1.0～1.4倍,但降低了土壤微生物的呼吸活性;培养后期,NaOH和H2SO4处理使土壤微生物量C分别下降了28%和42%,但增加了土壤微生物的呼吸活性;H2O₂处理则使土壤微生物量N增加90%;土壤微生物区系中的真菌比例在不同时刻有所增加.表明将秸秆预处理后施入土壤,将对土壤中微生物数量和呼吸活性产生一定影响.

Abstract: Winter wheat straw particles (0.5～2.0 mm) were soaked with 8.0 g·L^-1 H2O₂ (pH11.0),12.5 g·L^-1 NaOHor H2SO4 solution for 8 h and dried at 80 ℃. Soils amended with the pretreated straw and inorganic Nwere incubated aerobically at 25 ℃ for 60 days. The CO₂ emission rate and soil microbial biomass Cand Nwere measured at different time. The results showed that during the earlier stage of incubation,the pretreatments of straw increased soil microbial biomass Cby 1.0～1.4 folds,but decreased soil microbial respiration activity. During the later stage of incubation,the NaOHand H2SO4 pretreated straw decreased soil microbial biomass carbon by 28% and 42%,respectively,while increased the soil microbial respiration activity. The straw pretreated by H2O₂ increased soil microbial biomass nitrogen by 90% after the 15th day of incubation. The pretreatments of straw increased the fungi/bacteria ratio at different special time. It could be concluded that soil microbial biomass and respiration activity could be changed after the pretreated straw was added into the soil.

中图分类号:

S15.36

李贵桐, 张宝贵, 李保国. 秸秆预处理对土壤微生物量及呼吸活性的影响[J]. 应用生态学报, 2003, (12): 2225-2228.

LI Guitong, ZHANG Baogui, LI Baoguo . Effect of straw pretreatment on soil microbial biomass and respiration activity[J]. Chinese Journal of Applied Ecology, 2003, (12): 2225-2228.

参考文献

[1] Allison MF, Killham K. 1988. Response of soil microbial biomass to straw incorporation. J Soil Sci, 39(2): 237～242
[2] Amato M, Ladd JN. 1988. Assay for microbial biomass based on ninhydrin- Reactive nitrogen in extracts of fumigated soils. Soil Biol Biochem, 20(1): 107～114
[3] Anderson TH, Domsch KH. 1990. Application of eco-physiological quotients (qCO₂ and qD) on microbial biomass from soils of different cropping histories. Soil Biol Biochem, 22(2) :251～255
[4] Bonde TA, Rosswall T. 1987. Seasonal variation of potentially mineralizable nitrogen in cropping systems. Soil Sci SociAmer J, 51(6): 1508～1514
[5] Brookes PC, Landman A, Pruden G, et al. 1985. Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem, 17(6): 837～842
[6] Brookes PC, Newcombe AD, Jenkinson DS. 1987. Adenylate energy charge measurement in soils. Soil Biol Biochem, 19(2) :211～217
[7] Cheshire MV, Chapman SJ. 1996. Influence of the N and P status of plant material and of added N and P on the mineraliazation of C fron 14C-labelled ryegrass in soil. BiolFertilSoil, 21(3):166～170
[8] Chotte JL, Ladd JN, Amato M. 1998. Sites of microbial assimilation, and turnover of soluble and particulate 14C-labeled substrates decomposing in a clay soil. Soil Biol Biochem, 30(2) :205～218
[9] GonzalezG,Lopez-SantinJ,CaminalG, et al.1986.Dilute acidhydrolysis of wheat straw hemicellulose at moderate temperature: A simplified kinetic model. Biotechnol Bioengin, 28 (2): 288～293
[10] Gould JM. 1985a. Studies on the mechanism of alkaline peroxide delignification of agricultural residues. Biotechnol Bioengineer, 27(3) :225～231
[11] Gould JM. 1985b. Enhanced polysaccharide recovery from agricultural residues and perennial grasses treated with alkaline hydrogen peroxide. Biotechnol Bioengin, 27(6):893～896
[12] Knapp EB, Elliott LF, Campbell GS. 1983. Microbial respiration and growth during the decomposition of wheat straw. Soil Biol Biochem, 15(3) :319～324
[13] Ocio JA, Brookes PC. 1990. An evaluation of methods for measuring the microbial biomass in soils following recent additions of wheat straw, and the characterization of the biomass that develops.Soil Biol Biochem , 22(5): 685～694
[14] Patra DD, Bhandari SC, Misra A. 1992. Effect of plant residues on the size of microbial biomass and nitrogen mineralization in soil in corporation of cowpea and wheat straw. Soil Sci Plant Nutr, 38(1):1～6
[15] Paul EA, Clark FE. 1996. Soil Microbiology and Biochemistry. 2nd.New York: Academic Press. 184
[16] Pavlostathis SG, Gossett JM. 1985. Alkaline treatment of wheat straw for increasing anaerobic biodegradability. Biotechnol Bioengin, 27(3): 334～344
[17] Redl. G, Hubner C, Wurst F. 1990. Changes in hot water soil extracts brought about by nitrogen immobilization and mineralization processes during incubation of amended soils. Biol Fertil Soil, 10(1) :45～49
[18] Reinertsen SA, Elliott LF, Cochran VL, et al. 1984. The role of available C and N in determining the rate of wheat straw decomposition. Soil Biol Biochem, 16(5) :459～464
[19] Santruckova H, Straskaba M. 1990. On the relationship between specific respiration, activity and microbial biomass in soils. Soil Biol Biochem, 23(6): 525～531
[20] Vaccarino C, Tripodo MM, Curto RB, et al. 1993. The effects of NaOH treatments of grape-marc, vinasse, and wheat-straw mixtures on their degradability in vitro. Bioresour Technol, 41(2): 197～202