温州蜜柑叶片光合作用光抑制的保护机理

应用生态学报 ›› 2003, Vol. ›› Issue (1): 47-50.

温州蜜柑叶片光合作用光抑制的保护机理

宋丽丽¹, 郭延平², 徐凯², 张良诚²

1. 安徽农业大学园艺系, 合肥 230036;
2. 浙江大学园艺系, 杭州 310029

收稿日期:2001-11-05 修回日期:2002-05-15 出版日期:2003-01-15
通讯作者: 郭延平
基金资助:
国家自然科学基金(39970525);浙江省自然科学基金资助项目(399470).

Protective mechanism in photoinhibition of photosynthesis in Citrus unshiu leaves

SONG Lili¹, GUO Yanping², XU Kai², ZHANG Liangcheng²

1. Department of Horticulture, Anhui Agricultural University, Hefei 230036, China;
2. Department of Horticulture, Zhejiang University, Hangzhou 310029, China

Received:2001-11-05 Revised:2002-05-15 Online:2003-01-15

摘要/Abstract

摘要： 晴天条件下,使用便携式调制荧光仪和分光光度计观察了温州蜜柑叶片光合作用光抑制发生过程中几个主要荧光参数(初始荧光F₀、最大荧光Fm、PSÒ的光化学效率Fv/Fm、非光化学猝灭qN及其快相qN_f和慢相qN_s)、电子传递速率(ETR)和玉米黄素相对含量的日变化.结果表明,随着光强的增强,ETR、qN及其qN_f与qN_s以及玉米黄素相对含量升高,Fv/Fm、Fm和F₀下降.用DTT处理后,qN_s较对照明显下降,F0较对照明显上升.可以认为,柑橘在光合作用日变化中存在依赖于叶黄素循环和类囊体膜质子梯度两种非辐射能量耗散方式,而且它们在防御光破坏方面起着重要作用.

Abstract: By using a portable PAM-2000 fluorometer and a spectrophotometer (722 Model), the diurnal variation in main chlorophyll fluorescence parameters such as initial fluorescence (F₀), maximal fluorescence (Fm), ratio of variable fluorescence to maximal fluorescence (Fv/Fm), non-photochemical quenching (qN), fast-phase (qN_f) and slow-phase (qNs) of non-photochemical quenching, electron transport rate (ETR), and relative content of xeaxanthin in Citrus unshiu leaves were observed on fine days. The results showed that ETR, qN, qN_f, qN_s and relative content of xeaxanthin increased with increasing light intensity, but Fm, F₀, photo-chemical efficiency of PSÒ(Fv/Fm) decreased. After treated by DTT, qN_s was much lower, but F₀ was much higher than control. These results indicated that xanthophyll cycle and △pH existed,and played an important protective role against photo-damage to the photosynthetic apparatus in Citrus unshiu leaves during diurnal variation of photosynthetic efficiency.

中图分类号:

Q945.11

宋丽丽, 郭延平, 徐凯, 张良诚. 温州蜜柑叶片光合作用光抑制的保护机理[J]. 应用生态学报, 2003, (1): 47-50.

SONG Lili, GUO Yanping, XU Kai, ZHANG Liangcheng. Protective mechanism in photoinhibition of photosynthesis in Citrus unshiu leaves[J]. Chinese Journal of Applied Ecology, 2003, (1): 47-50.

参考文献

[1] Adams Ó WW ＆ Demmig-Adams B.1992.Operation of the xanthophyll cycle in higher plants in response to diurnal changes in incident sunlight.Planta,186:390～398
[2] Baker NR.1991.A possible role for photosystem Ò.Environmental pertubations of photosynthesis.Physiol Plant, 81:563～570
[3] Bilger W ＆ Bjrkman O.1990.Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis.Photosynth Res,25:173～185
[4] Bilger W, Bjrkman O ＆ Thayer SS.1989.Light-induced spectral absorbance changes in relation to photosynthesis and the epoxidation state of xanthophyll cycle components in cotton leaves.Plant Physiol,91:542～551
[5] Chen Z-H (陈志辉), Zhang L-C(张良诚).1994.Diurnal variation in photosynthetic efficiency of leaves in Satsuma Mandarin.Acta Phytophysiol Sin(植物生理学报),20(3):263～271(in Chinese)
[6] Demmig B, Winter K, Krüger A,et al.1987.Photoinhibition and zeaxanthin formation in intact leaves:A possible role of the xanthophyll cycle in the dissipation of excess light energy.Plant Physiol, 84:218～224
[7] Demmig-Adams B ＆ Adams Ó WW.1996.Xanthophyll cycle and light stress in nature:uniform response to excess direct sunlight among higher plant species.Planta,198:460～470
[8] Demmig-Adams B ＆ Adams Ó WW.1992.Photoprotection and other responses of plants to high light stress.Ann Rev Plant Physiol Plant Mol Biol,43:599～626
[9] Demmig-Adams B ＆ Winter K.1988.Characterization of three components of non-photochemical fluorescence quenching and their response to photoinhibition.Aust J Plant Physiol, 15:163～177
[10] Demmig-Adams B, Adams Ó WW, Baker DH,et al.1996.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.Physiol Plant,98:253～264
[11] Dodd IC, Critchley C, Woodall GS,et al.1998.Photoinhibition in differently colored juvenile leaves of Syzygium species.J Exper Bot,49(325):1437～1445
[12] Genty B, Briantais JM, Baker NR.1989.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.Biochem Biophys Acta, 990:87～92
[13] Guo Y-P(郭延平), Zhan L-C(张良诚), Hong S-S(洪双松),et al.1999.Photoinhibition of photosynthesis in Satsuma Mandarin (Citrus unshiu Marc.).Acta Hortic Sin(园艺学报), 26(5):281～286.(in Chinese)
[14] Hong Shuang-Song, Xu Da-Quan.1999.Light-induced increase in initial chlorophyll fluorescence F₀ level and the reversible inactivation of PSⅡreactivation centers in soybean leaves.Photosynth Res,61:269～280
[15] Hong T(洪涛), Xu D-Q (许大全).1999.Non-photochemical quenching of chlorophyll fluorescence in soybean and sweet viburnum leaves.Acta Phytophysiol Sin(植物生理学报), 25(1):15～21 (in Chinese)
[16] Horton P, Hague A.1988.Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts Ô.Resolution of non-photochemical quenching.Biochem Biophys Acta, 932:107～115
[17] Horton P, Ruban A, Walters RG.1994.Regulation of light havesting in green plants:indication by non-photochemical quenching of chlorophyll fluorescence.Plant Physiol, 106:415～423
[18] Powles SB.1984.Photoinhibition of photosynthesis induced by visible light.Annu Rev Plant Physiol, 35:15～44
[19] Quick WP, Stitt M.1989.An examination of factors contributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves.Biochem Biophys Acta,977:287～296
[20] Xu D-Q(许大全), Xu B-J(徐宝基), Shen Y-G(沈允钢).1990.Diurnal variation of photosynthetic efficiency in C3 plants.Acta Phytophysiol Sin(植物生理学报),16:1～5 (in Chinese)