薇甘菊挥发油的化学成分及其对昆虫的生物活性

应用生态学报 ›› 2003, Vol. ›› Issue (1): 93-96.

薇甘菊挥发油的化学成分及其对昆虫的生物活性

张茂新, 凌冰, 孔垂华, 庞雄飞, 梁广文

华南农业大学昆虫生态研究室, 广州 510642

收稿日期:2002-03-15 修回日期:2002-07-25 出版日期:2003-01-15
通讯作者: 张茂新,男,1957年生,博士,副教授,主要从事昆虫种群生态学和昆虫与植物协同进化研究.发表论文30篇.E-mail:mxzhang@scau.edu.cn.
基金资助:
国家自然科学基金重点项目(39930120);国家自然科学基金资助项目(39870441).

Chemical components of volatile oil from Mikania micrantha and its biological activity on insects

ZHANG Maoxin, LING Bing, KONG Chuihua, PANG Xiongfei, LIANG Guangwen

Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China

Received:2002-03-15 Revised:2002-07-25 Online:2003-01-15

摘要/Abstract

摘要： 用GC/MS分析了薇甘菊挥发油的化学成分,共鉴定了22个化合物,单萜和倍半萜及其醇和酮的衍生物是其主要成分,并研究了薇甘菊挥发油对昆虫的生物活性.结果表明,在5～10μl·株^-1的用量时,它们对小菜蛾、黄曲条跳甲和猿叶虫有显著的产卵驱避作用.同时也具有一定的触杀毒力,在500、750、1000mg·L^-1浓度时,对萝卜蚜的校正虫口减退率分别为50.0%、59.86%和62.51%.然而,在500mg·L^-1浓度时,对萝卜蚜、小菜蛾和黄曲条跳甲等不同受试虫态却无熏蒸毒杀作用.

Abstract: The chemical components of volatile oil from Mikania micrantha were analyzed by GC/MS. Twenty-two compounds were identified, and monoterpenes and sesquiterpenes as well as alcohol and ketone with their derivatives were major components. Biological activity of volatile oil on insect was investigated. The results showed that the volatile oil had significant deterrent effect on oviposition of Plutella xylostella, Phyllotreta striolata and Phaedon brassicae at dose of 5～10μl·plant^-1.They also possessed established contacting toxicity, and their rectifying reduce rates for Lipaphis erysimi were 50.0%, 59.86%, 62.51% at concentration of 500, 750, and 1000mg·L^-1,respectively.However, they had not fumigating toxicity for virginogeniae, L. erysimi, 2^nd instar larvae of P. xylostella, and adult of P. striolata at concentration of 500mg·L^-1.

中图分类号:

Q949.783.5

张茂新, 凌冰, 孔垂华, 庞雄飞, 梁广文. 薇甘菊挥发油的化学成分及其对昆虫的生物活性[J]. 应用生态学报, 2003, (1): 93-96.

ZHANG Maoxin, LING Bing, KONG Chuihua, PANG Xiongfei, LIANG Guangwen . Chemical components of volatile oil from Mikania micrantha and its biological activity on insects[J]. Chinese Journal of Applied Ecology, 2003, (1): 93-96.

参考文献

[1] Du Y-J(杜永均),Yan F-S(严福顺).1994.The role of plant volatiles in tritrophic interaction among phytophagous insects, their host plants and natural enemies.Acta Ent Sin(昆虫学报),37(2):233～250(in Chinese)
[2] Hu Y-J(胡玉佳),Bi P-X(毕培曦). 1994. A study on life cycle and response to herbicides of Mikania micrantha.Acta Sci Natur Univ Sunyatseni(中山大学学报,自然科学版),33(4):88～95(in Chinese)
[3] Huang Z-L(黄忠良),Cao H-L(曹洪麟),Liang X-D(梁晓东). 2000. The growth and damaging effect of Mikania micrantha in different habitats.J Trop Subtrop Bot(热带亚热带植物学报),8(2):131～138(in Chinese)
[4] Kong C-H(孔垂华) .2002.Frontier fields of plant chemical ecology in the 21st century.Chin J Appl Ecol (应用生态学报),13(3):349～353(in Chinese)
[5] Kong C-H, Hu F, Xu X. 2002. Allelopathic potential and chemical constituent of volatile from Ageratum conyzoides under stress.J Chem Ecol,28(6):1184～1195
[6] Kong C-H(孔垂华), Huang S-S(黄寿山), Hu F(胡飞). 2001. Allelopathy of Ageratum conyzoides V.Biological activities of the volatile oil from ageratum on fungi, insects and plants and its chemical constituents.Acta Ecol Sin(生态学报), 21(4):584～587(in Chinese)
[7] Kong G-H(孔国辉),Wu Q-G(吴七根),Hu Q-M(胡启明),et al. 2000. Further supplementary data on Mikania micrantha H. B. K. (Asteraceae) .J Trop Subtrop Bot(热带亚热带植物学报),8(1):128～130(in Chinese)
[8] Kong C-H(孔垂华), Xu T(徐涛), Hu F(胡飞).1998.Study on interactions among allelochemicals of Ageratum conyzoides. Acta Phytoecol Sin(植物生态学报), 22(5):403～408(in Chinese)
[9] Lungren L. 1975. Natural plant chemicals acting as oviposition deterrents on cabbage butterflies (Pieris brassicae, P. rapae, P. napi). Zool Sci, 4:253～258
[10] Mu L-Y(慕立义). 1994. Research method of plant chemical protection. Beijing: China Agricultural Press . 55～56(in Chinese)
[11] Nottingham SF, Haedie AJ. 1993. Flight behavior of the black bean aphid Aphis fabae and the cabbage aphid Brevicoryne brassicae in host and nonhost plant odor.Physiol Entomol,18:389～394
[12] Nottingham SF, Haedie AJ,Dowson GW,et al. 1991.Behavioral and electrophysiogical responses of aphids to host and nonhost plant volatiles.J Chem Ecol,17:1231～1242
[13] Nicollier G, Thompson AC.1981. Essential oil and terpenoids of Mikania micrantha. Phytochemitry,20:2587～2588
[14] Petrea C, Facey KO, Pascoe RB,et al. 1999. Investigation of plants used in Jamaican folk medicine for anti-bacterial activity.J Pharm Pharmacol,51:1455～1460
[15] Qing J-D(钦俊德).1987.Insect and Plant-The Evolution and Interaction of Insect and Plant. Beijing: Science Press.38～61(in Chinese)
[16] Terrance DH, David FW. 1985. Anti-repellent terpenoides from Mikania micrantha.Phytochemitry, 24:1197～1198
[17] Tingle FC, Michell ER. 1991.Effect of oviposition deterrents from elderberry on behavioral responses by Heliothis virescens to host-plant volatiles in flight tunnel.J Chem Ecol,17(8):1621～1631
[18] Zhang M-X(张茂新),Liang G-W(梁广文). 2000. Design and apply of egg separating device for Phyllotreta striolata(F.)(Coleoptera:Chrysomelidea).Entom Knowle(昆虫知识),37 (3):173～174(in Chinese)
[19] Zhng M-X(张茂新),Ling B(凌冰),Kong C-H(孔垂华),et al.2002.Allelopathic potential of volatile oil from Midania micrantha.Chin J Appl Ecol(应用生态学报),13(10):1300～1302(in Chinese)