Abstract: Quantitative analysis of vessel characteristics at the cellular scale is of great significance for understan-ding plant adaptation strategies to environment. The direct grinding combined with stereo-microscope imaging is one of the main approaches to examine the anatomical structure of xylem (conifer tracheid and hardwood vessel) wood structure, which inevitably damages xylem cells, hindering the accurate understanding of anatomical structures. In this study, we applied X-ray micro-computed tomography (μCT) and stereo-microscope technology to quantitatively measure the diameter and area of vessels of seven Canadian broadleaved tree species (Acer saccharum, Betula papyrifera, Fraxinus americana, Ostrya virginiana, Populus grandidentata, Quercus rubra, and Carya cordiformis). We fitted the results by linear model and tested the feasibility of μCT technology in quantifying the vessel size of broadleaved species. We found that the results of the two methods for measuring vessel size were highly similar (R²=0.98). The goodness of fit of the vessel diameter results measured by the two methods for the ring-porous wood species (C. cordiformis, R²=0.98; F. americana, R²=0.96; Q. rubra, R²=0.99) was higher than that of the diffuse-porous wood species (B. papyrifera, R²=0.88; O. virginiana, R²=0.73; A. saccharum, R²=0.68; P. grandiden-tata, R²=0.88). The goodness of fit of small vessels (diameter≤200 μm, R²=0.94) measured by the two methods was higher than that of large vessels (diameter>200 μm, R²=0.92). Thus, the μCT technique provided a new non-destructive detection method for quantifying xylem vessels of broadleaved tree species.

Key words: xylem, vessel, Micro-CT, broadleaved tree