Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species

doi:10.13287/j.1001-9332.202501.016

Abstract

Abstract: Soil dissolved organic matter (DOM) is an important component of soil carbon pool, and its interaction with soil fungal communities is crucial for soil carbon cycle. We set up three nitrogen (N) addition levels, including control (0 kg N·hm^-2·a^-1), low N (40 kg N·hm^-2·a^-1), and high N (80 kg N·hm^-2·a^-1), in Pinus taiwanensis and Castanopsis faberi forests to explore the effect of N addition on the DOM quantity and optical properties, as well as the relationship between soil fungal communities and DOM by using three-dimensional fluorescence spectroscopy coupled with parallel factor analysis and high-throughput sequencing. The results showed that N addition did not significantly change the quantity or optical properties of soil DOM and Chao1 index in P. taiwanensis forest. In contrast, N addition, especially the high addition rate, significantly reduced the content of soil dissolved organic carbon, humification index, and relative content of humic-like components of DOM in C. faberi forest. Additionally, the relative abundance of Mortierellomycota in the fungal community significantly increased after N addition. Redundancy analysis indicated that soil DOM content and optical properties in P. taiwanensis forest were mainly influenced by Chao1 index after N addition. In contrast, soil fungal community structure, β-glucosidase activity, and pH were the main factors influencing DOM content and optical properties in C. faberi forests. This suggested that the key factors affecting soil DOM quantity and optical properties after short-term N addition depended on tree species. Furthermore, network analysis demonstrated a close relationship between soil fungal communities and DOM components. Fewer associative edges were observed, suggesting that the association between soil fungal communities and DOM components was weakened by N addition. Overall, this study provided novel insights into the relationship between fungal communities and dissolved organic carbon pools in forest soils with N addition.

Key words: nitrogen deposition, tree species, dissolved organic matter, fungal community, co-occurrence network

BAI Xinyu, YUAN Xiaochun, ZENG Quanxin, ZHANG Xiaoqing, SUN Hao, ZHANG Qiufang, LU Jiaohong, CUI Juyan, CHEN Yuemin. Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species[J]. Chinese Journal of Applied Ecology, 2025, 36(1): 67-76.

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