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Chinese Journal of Applied Ecology ›› 1994, Vol. 5 ›› Issue (2): 156-158.

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Construction of Sporocytophaga B29 gene library and clouiug of CMCase gene

ZhangLixin1, Liu Chunqiang2, Kuai Jun2, Gao Peiji2   

  1. 1. Institute of Applied Ecology, Academia Sinica, Shenyang 110015;
    2. Institute of Microbiology, Shandong University, Jinan 250100
  • Received:1993-08-12 Revised:1994-01-26 Online:1994-04-25 Published:1994-04-25

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Abstract: An endoglucanase(CMCase)gene from Sporocytophaga B29 was cloned in Escherichia coli JM83. The chromosomal DNA of B29 was partially digested with restriction enzyme PstI and ligated into the plasmid pUC8 that had been linearized with the same enzyme.After transformation of JM83,the gene library of B29 was constructed and an endoglucanase-positive clone was detected in situ.All transformants with Apr had the activity of CMCase,proving that what we cloned was a CMCase gene.

Key words: Genetic engineering ecology, Sporocytophaga B29 gene library, CMCase gene clone, runoff., biological soil crust, surface roughness, revegetated grassland, infiltration