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应用生态学报 ›› 1998, Vol. 9 ›› Issue (6): 627-630.

• 研究论文 • 上一篇    下一篇

久效磷对扁藻的损伤作用 Ⅰ扁藻细胞的活性氧伤害作用

唐学玺1, 颜挺进2, 李永祺1   

  1. 1. 青岛海洋大学生命学院, 青岛266003;
    2. 山东省农业科学院作物研究所
  • 收稿日期:1997-04-02 修回日期:1997-10-16 出版日期:1998-11-25 发布日期:1998-11-25
  • 基金资助:

    国家科委PD B6(PD B6-7-1);山东省自然科学基金(Y94D0335)资助项目

Damage effect of monocrotophos on Platymonas spⅠ. Active oxygen in Platymonas spcells

Tang Xuexi1, Yan Tingjin2, Li Yongqi1   

  1. Qingdao Oceanology University, Qingdao 266003
  • Received:1997-04-02 Revised:1997-10-16 Online:1998-11-25 Published:1998-11-25

摘要: 以扁藻为材料,研究久效磷对扁藻细胞的毒性效应。结果表明,随着培养液中久效磷浓度的加大,扁藻细胞内的活性氧含量增加,膜脂过氧化产物丙二醛的含量与膜的通透性也相应地上升;同时,扁藻细胞内清除活性氧的2种关键性酶(过氧化物酶和超氧化物歧化酶)的活性逐渐降低,表明在久效磷的胁迫下,两种酶活性的降低打破了扁藻细胞内活性氧产生与清除间的正常平衡状态,使活性氧过量产生并积累。过量活性氧引起扁藻膜脂过氧化程度的加重和膜通透性的增加,从而对扁藻形成伤害。

关键词: 扁藻, 活性氧, 久效磷, 膜脂过氧化, 水分有效性, 克隆整合, 干旱区, 胡杨, 稳定同位素, 光合活性

Abstract: Studies on the toxicity effect of monocrotophos on Platymonas sp.cells show that with increasing concentration of monocrotophos culture medium, the content of active oxygen in Platymonas sp.cells increased obviously, and correspondingly, that of malondialdehyde(MDA), peroxidation product of membrane lipid, and membrane permeability also increased.At the meanwhile, the activity of peroxidase(POD)and superoxide dismutase(SOD), two key enzymes for eliminating active oxygen, decreased gradually.These results indicate that under monocrotophos stress, the decline of PODand SODactivities broke the normal balance between production and eliminating of active oxygen, making active oxygen excessive and accumulated.The excessive active oxygen resulted in the aggravation of lipid peroxidation and the increase of membrane permeability, and therefore, the damage of Platymonas sp.appeared.

Key words: Platymonas sp, Active oxygen, Monocrotophos, Membrane lipid peroxidation, water availability, clonal integration, arid region, Populus euphratica, stable isotope, photosynthetic activity