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云南六种实蝇的RAPD快速鉴定

张亮;张智英   

  1. 云南大学生态学与地植物学研究所, 昆明 650091
  • 收稿日期:2006-03-28 修回日期:1900-01-01 出版日期:2007-05-15 发布日期:2007-05-15

Random amplified polymorphic DNA identification of six Bactrocera (Diptera:Tephritidae) species in Yunnan Province of Southwest China.

ZHANG Liang; ZHANG Zhi-ying   

  1. Institute of Ecology and Geobotany, Yunnan University, Kunming 650091,China
  • Received:2006-03-28 Revised:1900-01-01 Online:2007-05-15 Published:2007-05-15

摘要: 采用RAPD技术构建了果实蝇属(Bactrocera)的南瓜实蝇(B. tau)、黑漆实蝇(B.scutellaris)、具条实蝇(B. scutellata)、瓜实蝇(B. cucurbitae)、桔小实蝇(B. dorsalis)和番石榴实蝇(B. correcta)6种实蝇的指纹图谱.从131个引物中筛选出5个重复性好、多态性高的引物,共扩增出302条谱带,其中111条谱带具有遗传多态性.引物OPC-01、OPI-17、OPL-07、OPL-08以及OPL-16可以用于6种实蝇的分类鉴定.

关键词: 农林间作, 32P, 分配, 迁移

Abstract: In this paper, the genetic relationships among six fruit fly species of Bactrocera in Yunnan Province of Southwest China, i. e. , B. tau, B. scutellaris, B. scutellata, B. cucurbitae, B. dorsalis and B. correcta, were studied by using the technique of random amplified polymorphic DNA (RAPD). Five primers with good repetition and high polymorphism were screened from 131 primers, and a total of 302 bands were amplified, among which, 111 bands were of genetic polymorphism. The primers OPC-01, OPI-17, OPL-07, OPL-08 and OPL-16 could be used for the identification of the 6 fruit fly species.

Key words: Agroforestry ecosystem, 32P, Distribution, Translocation