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高温胁迫下外源钙对菊花叶片光合机构与活性氧清除酶系统的影响

孙宪芝1;郭先锋2;郑成淑1;王文莉1;梁芳1   

  1. 1山东农业大学园艺科学与工程学院作物生物学国家重点实验室, 山东泰安 271018; 2山东农业大学林学院, 山东泰安 271018
  • 收稿日期:2007-12-28 修回日期:1900-01-01 出版日期:2008-09-20 发布日期:2008-09-20

Effects of exogenous Ca2+ on leaf photosynthetic apparatus and active oxygen scavenging enzyme system of chrysanthemum under high temperature stress.

SUN Xian-zhi1;GUO Xian-feng2;ZHENG Cheng-shu1;WANG Wen-li1;LIANG Fang1   

  1. 1State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong China;2College of Forestry, Shandong Agricultural University, Tai’〖KG-*3〗an 271018, Shandong, China
  • Received:2007-12-28 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

摘要: 以切花菊品种‘神马’为材料,研究了高温胁迫下外源Ca2+对菊花光合系统及活性氧清除酶系统的影响,探讨了Ca2+可能的作用机制.结果表明:高温胁迫下外源Ca2+抑制了净光合速率(Pn)与实际量子效率(ΦPSⅡ)的大幅度降低,24 h后,二者分别比对照高出31.11%与21.88%,而初始荧光(Fo)比对照降低了13.19%;Ca2+明显激活了高温胁迫下叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,活性氧得到及时清除,24 h后,膜脂过氧化产物丙二醛(MDA)的积累量与相对电导率(REC)分别比对照降低29.20%与35.81%,缓解了短期高温胁迫对菊花光合系统的破坏.

关键词: 溶酶体, 污染, 生物标志物, 溶酶体膜稳定性, 中性红保留时间

Abstract: Taking cut flower chrysanthemum ‘Jinba’ as test material, the effects of exogenous Ca2+ on its photosynthetic system and antioxidant enzyme activities under high temperature stress were investigated, with the possible action mechanisms of Ca2+ discussed. The results showed that under high temperature stress, Ca2+ addition greatly inhibited the net photosynthesis rate (Pn) and quantum yield of PSⅡ electron transport (ΦPSⅡ). After 24 h treatment, the Pn and ΦPSⅡ were increased by 31.11% and 21.88%, respectively, and the initial fluorescence (Fo) decreased by 13.19%, compare with the control. Ca2+ addition also greatly enhanced the activities of SOD, POD and CAT, and thus, the active oxygen was scavenged timely. After 24 h treatment, the MDA accumulation and REC were 29.20% and 35.81% lower than the control, respectively. In conclusion, Ca2+ addition could efficiently protect chrysanthemum leaves from the damage in photosynthetic apparatus under shortterm high temperature stress.

Key words: lysosome, pollution, biomarker, lysosomal membrane stability, neutral red retention time.