关键词: 碱菀, 盐碱和干旱胁迫, 种子发芽, Na⁺/H⁺逆向转运蛋白基因, 非结构性碳水化合物, 枝条, 叶, 胡桃楸, 春榆, 尺度上推

Abstract: By applying 40-400 mmol·L^-1 NaCl, 20-200 mmol·L^-1 NaHCO_3, and 5%-30% PEG, this paper studied the effects of saline-alkali and drought stress on the seed germination and Na⁺/H⁺ antiporter gene (TvNHX1) expression of Tripolium vulgare.40-160 mmol·L^-1NaCl, 20 mmol·L^-1NaHCO_3, and 5%-10% PEG-6000 had less effects on the seed germination; while ≥240 mmol·L^-1 NaCl decreased the seed germination rate,  root length, and shoot length (P<0.05), ≥50 mmol·L^-1NaHCO₃  decreased the seed germination rate (P<0.05), 130 mmol·L^-1NaHCO₃ decreased the seed germination potential, root length, and shoot length (P<0.05), and ≥15% PEG delayed the seed germination. TvNHX1transcripts were basically constitutive at germination stage, but their expression increased distinctly at 160 mmol·L ^-1 NaCl, 100 mmol·L^-1 NaHCO₃, and 10% PEG. Under salt and drought stress, the expression of TvNHX1changed inphase with the phenotype change of germinated seeds, suggesting that  TvNHX1 play important roles in the tolerance of  T. vulgare against adversity stress.

Key words: Tripolium vulgare, saline-alkali and drought stress, seed germination, TvNHX1, nonstructural carbohydrates, branch, leaf, Juglans mandshurica, Ulmus japonica, up-scaling.