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应用生态学报 ›› 2003, Vol. ›› Issue (8): 1287-1290.

• 研究论文 • 上一篇    下一篇

日光温室光温因子对黄瓜叶绿体超微结构及其功能的影响

张振贤1, 郭延奎2, 艾希珍2, 张福墁1, 何启伟3, 孙小镭3, 焦志高3   

  1. 1. 中国农业大学园艺学院, 北京 100094;
    2. 山东农业大学, 泰安 271018;
    3. 山东省农业科学院蔬菜研究所, 济南 250100
  • 收稿日期:2001-03-09 修回日期:2001-08-29
  • 通讯作者: 张振贤,男,1956年生,博士,教授,主要从事蔬菜分子生物学生理与分子生物学研究,已发表学术论文60余篇.E-mail:Zhangzx@cau.edu.cn.
  • 基金资助:
    山东省“三O”工程;国家自然科学基金重点资助项目(39830230)

Effects of sunlight and temperature on ultrastructure and functions of chloroplast of cucumber in solar greenhouse

ZHANG Zhenxian1, GUO Yankui2, AI Xizhen2, ZHANG Fuman1, HE Qiwei3, SUN Xiaolei3, JIAO Zhigao 3   

  1. 1. College of Horticulture, China Agricultural University, Beijing 100094, China;
    2. Shandong Agricultural University, Taian 271018, China;
    3. Institute of Vegetable, Shandong Academy of Agricultural Science, Jinan 250100, China
  • Received:2001-03-09 Revised:2001-08-29

摘要: 在日光温室内,研究了光温因子对黄瓜叶绿体超微结构及其功能的影响.结果表明,因季节之间光、温条件不同,日光温室黄瓜叶片显微结构和叶绿体超微结构有一定差异,1月份光照弱叶肉细胞较大,而5月份光照强叶绿体数较多.在该试验条件下,未发现叶片光合速率与叶绿体超微结构之间有直接或密切的相关性.在各生长季节其光合速率均为第4叶>初展叶>基部叶,与叶龄及各叶位的受光量有关.如果将不同叶位叶放在相同的光照下,则差异明显减少.黄瓜叶片的叶肉细胞、叶绿体和淀粉粒的大小以及叶绿体数、基粒数、基粒厚度、基粒片层数都随叶位的下降而呈增加趋势,不同品种、同品种不同生长时期的叶片显微结构和叶绿体超微结构及其功能也有一定的差异.限制日光温室冬季黄瓜光合作用的主要因素是光照弱、有效光照时数少,而在晴天温度的限制作用相对较小,阴天因光照弱而导致的室内低温则是限制黄瓜生长的关键因素.

关键词: 黄瓜, 温光因子, 日光温室, 叶绿体超微结构, 基粒

Abstract: Studies on the effects of sunlight and temperature on the ultrastructure and functions of leaf chloroplasts of cucumber (Cucumis sativus L.) in solar greenhouse showed that the size of cells, chloroplasts, and starch grains and the number of chloroplast, grana, grana lamella and grana thickness of leaf chloroplast of cucumber increased with lowering leaf position. The microstructure of leaves and the ultrastructure of chloroplast of cucumber leaves were different because of the difference of temperature and sunlight among different months. In January, the light intensity was lower,and the cell size of cucumber leaves was larger than that in May, but the number of chloroplasts was less than that in May. The relationships between photosynthetic rate (Pn) and ultrastructure of chloroplast of cucumber leaves were not direct and very close. The Pn of the fourth leaf of cucumber was the highest, that of the first spreading out leaf was the second, and the near ground leaves had the lowest Pn.The primary reasons of the difference of Pn among different position leaves were that the leaf age and accepted light intensity were different. If different position leaves of cucumber accepted the same sunlight intensity, the difference of Pn among leaves of different positions would be smaller. Less efficient light hours and lower light intensity were the main factors influencing cucumber growth and yield in solar greenhouse. Comparatively, under sunny circumstance,the restriction of temperature on cucumber growth was very small, but in cloudy days, lower temperature induced by low light intensity became a key factor that restricted cucumber growth.

Key words: Cucumber, Temperature and sunlight, Solar greenhouse, Ultrastructure of chloroplast, Grana

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