生物质调理剂对川西北高寒草地沙化土壤养分和微生物群落特征的影响

doi:10.13287/j.1001-9332.202106.036

应用生态学报 ›› 2021, Vol. 32 ›› Issue (6): 2217-2226.doi: 10.13287/j.1001-9332.202106.036

生物质调理剂对川西北高寒草地沙化土壤养分和微生物群落特征的影响

宋佳珅¹, 张晓丽¹, 孔凡磊², 刘晓林², 安文静³, 李玉义^1*

¹中国农业科学院农业资源与农业区划研究所, 北京 100081;
²四川农业大学农学院/农业部西南作物生理生态与耕作重点实验室, 成都 611130;
³北京京西林场, 北京 102300

收稿日期:2020-09-18 接受日期:2021-03-04 发布日期:2021-12-15
通讯作者: * E-mail: liyuyi@caas.cn
作者简介:宋佳珅, 女, 1997年生, 硕士研究生. 主要从事土壤耕作与土壤改良的研究. E-mail: sjs1119870671@163.com
基金资助:
国家科技支撑计划项目(2015BAC05B05)资助

Effects of biomass conditioner on soil nutrient and microbial community characteristics of alpine desertified grassland in northwest Sichuan, China

SONG Jia-shen¹, ZHANG Xiao-li¹, KONG Fan-lei², LIU-Xiao-lin², AN Wen-jing³, LI Yu-yi^1*

¹Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
²College of Agriculture, Sichuan Agricultural University / Key Laboratory of Crop Eco-physiology and Farming System in Southwest China, Chengdu 611130, China;
³Beijing Jingxi Forestry Farm, Beijing 102300, China

Received:2020-09-18 Accepted:2021-03-04 Published:2021-12-15
Contact: * E-mail: liyuyi@caas.cn
Supported by:
National Science and Technology Support Program of China (2015BAC05B05).

摘要/Abstract

摘要： 将农牧废弃物进行资源化处置制成生物质调理剂,用于沙化土壤改良是目前川西北沙化草地生态治理的有效途径之一。为了阐明不同原料调理剂在川西北高寒沙化草地上的实际应用效果,本研究以不施用调理剂为对照(CK),设置施用量均为12 t·hm^-2的菌渣(JZ)、秸秆(JG)和生物炭(SWT) 3种调理剂,分析了调理剂施用对土壤养分和微生物群落特征及其相互关系的影响。结果表明: 与对照相比,各种生物质调理剂均显著增加了土壤速效养分和活性有机碳含量,增幅在23.0%～521.6%,其中施用秸秆调理剂(JG)的效果最佳,增幅为65.1%～521.6%。因生物质调理剂只在第一年施入,第二年的土壤速效养分和活性有机碳含量均较第一年整体下降了4.5%～92.3%,而土壤有机碳含量和微生物生物量碳均较第一年整体上升了5.6%～458.0%。生物质调理剂改变了微生物属水平优势菌属的相对丰度,其中,JG处理对细菌菌群的影响较大,JG和JZ处理对真菌菌群的影响较大。与CK相比,JG处理显著降低了土壤细菌和真菌的多样性,其中,Shannon指数显著降低了2.9%和31.8%,而Simpson指数显著提高了175.0%和320.9%。冗余分析显示,土壤速效养分和活性有机碳含量是影响微生物群落组成的重要因素,其中,土壤硝态氮含量和微生物生物量碳对细菌群落组成的影响较大,共解释了65.9%的群落变化,土壤速效钾含量和微生物生物量碳对真菌群落组成的影响较大,共解释了83.2%的群落变化。综合比较,秸秆调理剂能显著增加土壤速效养分和活性有机碳含量,有利于有益细菌和真菌微生物菌属的生长,可以作为提升川西北地区高寒草地沙化土壤质量的推广措施。

关键词: 高寒草地, 沙化土壤, 生物质调理剂, 土壤养分, 土壤微生物群落

Abstract: Biomass conditioner made from agricultural and animal husbandry waste for resource disposal could be used to improve desertified soil, which is one of the effective ways of ecological management on desertified grasslands in northwest Sichuan. To clarify the effects of different raw material conditioners on alpine desertified grassland in northwest Sichuan, we analyzed the effects of three conditioners on soil nutrients and microbial community characteristics. With no conditioner as the control (CK), three different biomass conditioners were set up with an application rate of 12 t·hm^-2, including mushroom dregs (JZ), straw (JG) and biochar (SWT). The results showed that all biomass conditioners could significantly increase soil available nutrients and active organic carbon by 23.0%-521.6%. Among the three conditioners, JG had the best effect, with an improvement range for soil nutrient and organic carbon of 65.1%-521.6%. Because biomass conditioner was only applied in the first year, soil available nutrients and active organic carbon in the second year decreased by 4.5%-92.3% compared with that of the first year, while soil organic carbon and microbial biomass carbon content of the second year increased by 5.6%-458.0%. The biomass conditioners changed the relative abundance of the dominant bacteria in the microbial community. JG significantly affected bacterial flora, while JG and JZ affected fungal flora. Compared with CK, JG significantly reduced the diversity of soil bacteria and fungi. The Shannon index was decreased by 2.9% and 31.8%, while the Simpson index was increased by 175.0% and 320.9%, respectively. Results of the redundancy analysis showed that the contents of soil available nutrients and active organic carbon were important factors affecting microbial community composition. The contents of soil nitrate and microbial biomass carbon had greater impacts on bacterial community composition, explaining 65.9% of community variations. The contents of soil available potassium and microbial biomass carbon had a greater impact on fungal community composition, explaining 83.2% of community variation. According to the comprehensive comparison, straw conditioner could significantly increase soil available nutrients and active organic carbon, and benefit the growth of beneficial bacteria and fungi, which could be used as a promotion measure to improve soil quality of alpine desertified grassland in northwest Sichuan.

Key words: alpine grassland, desertified soil, biomass conditioner, soil nutrient, soil microbial community

宋佳珅, 张晓丽, 孔凡磊, 刘晓林, 安文静, 李玉义. 生物质调理剂对川西北高寒草地沙化土壤养分和微生物群落特征的影响[J]. 应用生态学报, 2021, 32(6): 2217-2226.

SONG Jia-shen, ZHANG Xiao-li, KONG Fan-lei, LIU-Xiao-lin, AN Wen-jing, LI Yu-yi. Effects of biomass conditioner on soil nutrient and microbial community characteristics of alpine desertified grassland in northwest Sichuan, China[J]. Chinese Journal of Applied Ecology, 2021, 32(6): 2217-2226.

参考文献

[1] 胡连通, 毛英, 胡进耀, 等. 川西北高寒沙化草地修复过程中植物群落变化特征. 四川林业科技, 2019, 40(5): 11-16 [Hu L-T, Mao Y, Hu J-Y, et al. Chara-cteristics of plant community changes during the restoration of alpine desertified grassland in Northwest Sichuan. Journal of Sichuan Forestry Science and Technology, 2019, 40(5): 11-16]
[2] 舒向阳, 胡玉福, 蒋双龙, 等. 川西北沙化草地植被群落、土壤有机碳及微生物特征. 草业学报, 2016, 25(4): 45-54 [Shu X-Y, Hu Y-F, Jiang S-L, et al. Plant community characteristics, soil organic carbon and soil biological properties of grassland desertification sites in Northwest Sichuan. Acta Prataculturae Sinica, 2016, 25(4): 45-54]
[3] 刘世贵, 葛绍荣, 龙章富. 川西北退化草地土壤微生物数量与区系研究. 草业学报, 1994, 3(4): 70-76 [Liu S-G, Ge S-R, Long Z-F. Studies on soil microorganism numbers and microbiota of degenerated rangelands in Northwest region of Sichuan, China. Acta Prata-culturae Sinica, 1994, 3(4): 70-76]
[4] Zhao HL, He YH, Zhou RL, et al. Effects of desertification on soil organic C and N content in sandy farmland and grassland of Inner Mongolia. Catena, 2008, 77(3): 187-191
[5] 王梦雅, 符云鹏, 黄婷婷, 等. 等碳量添加不同有机物料对土壤有机碳组分及土壤呼吸的影响. 中国烟草学报, 2018, 24(2): 65-73 [Wang M-Y, Fu Y-P, Huang T-T, et al. Effects of organic material application on organic carbon in and respiration of soil. Acta Tabacaria Sinica, 2018, 24(2): 65-73]
[6] 宋君祥, 雷雄, 叶明清, 等. 生物有机肥对川西北退化草地群落结构和生产性能的影响. 草地学报, 2019, 27(5): 1355-1363 [Song J-X, Lei X, Ye Q-M, et al. Effects of bioorganic fertilizer on community diversity and production performance of degraded grassland. Acta Prataculturae Sinica, 2019, 27(5): 1355-1363]
[7] Mary B, Recous S, Darwis D, et al. Interactions between decomposition of plant residues and nitrogen cycling in soil. Plant and Soil, 1996, 181: 71-82
[8] 赵亚丽, 郭海斌, 薛志伟, 等. 耕作方式与秸秆还田对土壤微生物数量、酶活性及作物产量的影响. 应用生态学报, 2015, 26(6): 1785-1792 [Zhao Y-L, Guo H-B, Xue Z-W, et al. Effects of tillage and straw returning on microorganism quantity, enzyme activities in soils and grain yield. Chinese Journal of Applied Ecology, 2015, 26(6): 1785-1792
[9] 车建美, 刘波, 张彦, 等. 菌渣对黑麦草根际微生物群落的影响. 草业科学, 2016, 33(10): 1942-1950 [Che J-M, Liu B, Zhang Y, et al. Effect of edible fungus residue on the ecological characteristics of microorganism in the rhizosphere of ryegrass. Pratacultural Science, 2016, 33(10): 1942-1950
[10] 许云翔, 何莉莉, 陈金媛, 等. 生物炭对农田土壤氨挥发的影响机制研究进展. 应用生态学报, 2020, 31(12): 4312-4320 [Xu Y-X, He L-L, Chen J-Y, et al. Effects of biochar on ammonia volatilization from farmland soil: A review. Chinese Journal of Applied Ecology, 2020, 31(12): 4312-4320]
[11] Zheng JF, Chen JH, Pan GX, et al. Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from Southwest China. Science of the Total Environment, 2016, 571: 206-217
[12] Anderson CR, Hamonts K, Clough TJ, et al. Biochar does not affect soil N-transformations or microbial community structure under ruminant urine patches but does alter relative proportions of nitrogen cycling bacteria. Agriculture, Ecosystems & Environment, 2014, 191: 63-72
[13] 张晓丽, 孔凡磊, 刘晓林, 等. 生物质改良剂对川西北地区高寒草地沙化土壤有机碳特征的影响. 中国生态农业学报, 2019, 27(11): 1732-1743 [Zhang X-L, Kong F-L, Liu X-L, et al. Effects of different biomass amendments on soil organic carbon characteristics in alpine desertification grassland of Northwest Sichuan. Chinese Journal of Eco-Agriculture, 2019, 27(11): 1732-1743
[14] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科学出版社, 2000: 147-195 [Lu-R-K. Soil and Agricultural Chemisry Analysis. Beijing: China Agricultural Science and Technology Press, 2000: 147-195]
[15] Bokulich NA, Subramanian S, Faith JJ, et al. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nature Methods, 2012, 10: 57-59
[16] Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Research, 2013, 41: 590-596
[17] 张晓丽, 张宏媛, 卢闯, 等. 河套灌区不同秋浇年限对土壤细菌群落的影响. 中国农业科学, 2019, 52(19): 3380-3392 [Zhang X-L, Zhang H-Y, Lu C, et al. Effects of the different autumn irrigation years on soil bacterial community in Hetao Irrigation District. Scientia Agricultura Sinica, 2019, 52(19): 3380-3392]
[18] 董亮, 孙泽强, 王学君, 等. 秸秆全量还田下氮肥调控对小麦产量及土壤不同形态钾的影响. 中国农学通报, 2014, 30(36): 37-41 [Dong L, Sun Z-Q, Wang X-J, et al. Effects of nitrogen fertilizer regulation with straw-return on wheat yield and potassium forms. Chinese Agricultural Science Bulletin, 2014, 30(36): 37-41]
[19] 刘晓林, 陈伟, 吴雅薇, 等. 秸秆颗粒改良剂对川西北高寒沙地土壤氮素和黑麦草生长的影响. 水土保持学报, 2018, 32(6): 229-235 [Liu X-L, Chen W, Wu Y-W, et al. Effects of granulated straw amendment on soil nitrogen and the growth of ryegrass in the alpine sandy land of Northwest Sichuan. Journal of Soil and Water Conservation, 2018, 32(6): 229-235]
[20] 张忠学, 李铁成, 齐智娟, 等. 控制灌溉下秸秆还田对稻田土壤氮素组成的影响. 农业机械学报, 2019, 50(11): 229-238 [Zhang Z-X, Li T-C, Qi Z-J, et al. Effects of straw returning on nitrogen composition in paddy soil under controlled irrigation. Transactions of the Chinese Society for Agricultural Machinery, 2019, 50(11): 229-238]
[21] 袁颖红, 黄静, 周际海, 等. 改良剂对旱地红壤水分特征和作物产量的影响. 水土保持学报, 2016, 30(1): 171-177 [Yuan Y-H, Huang J, Zhou J-H, et al. Effects of soil amendments on water characteristics and crop yields in upland red soil. Journal of soil and Water Conservation, 2016, 30(1): 171-177]
[22] He YT, Zhang WJ, Xu MG, et al. Long-term combined chemical and manure fertilizations increase soil organic carbon and total nitrogen in aggregate fractions at three typical cropland soils in China. Science of the Total Environment, 2015, 532: 635-644
[23] Zhang P, Chen XL, Wei T, et al. Effects of straw incorporation on the soil nutrient contents, enzyme activities, and crop yield in a semiarid region of China. Soil and Tillage Research, 2016, 160: 65-72
[24] 王学霞, 张磊, 梁丽娜, 等. 秸秆还田对麦玉系统土壤有机碳稳定性的影响. 农业环境科学学报, 2020, 39(8): 1774-1782 [Wang X-X, Zhang L, Liang L-N, et al. Effects of straw returning on the stability of soil organic carbon in wheat-maize rotation systems. Journal of Agro-Environment Science, 2020, 39(8): 1774-1782]
[25] Blair GJ, Lefroy RDB. Soil carbon fractions based on their degree of oxidation and the development of a carbon management index for agricultural systems. Australian Journal Agricultural Research, 1995, 46: 1459-1466
[26] 龚臣, 王旭东, 倪幸, 等. 长期菌渣化肥配施对稻田土壤活性有机碳组分和有效养分的影响. 浙江农林大学学报, 2018, 35(2): 252-260 [Gong C, Wang X-D, Ni X, et al. Effects of long-term application of edible fungus residue and chemical fertilizers on fractions of labile organic carbon and available nutrients in rice field soils. Journal of Zhejiang A&F University, 2018, 35(2): 252-260]
[27] Xu MG, Lou YL, Sun XL, et al. Soil organic carbon active fractions as early indicators for total carbon change under straw incorporation. Biology & Fertility of Soils, 2011, 47: 745-752
[28] 刘怡萱, 曹鹏熙, 马红梅, 等. 青藏高原土壤微生物多样性及其影响因素研究进展. 环境生态学, 2019, 1(6): 1-7 [Liu Y-X, Cao P-X, Ma H-M, et al. Research progress on soil microbial diversity and its influen-cing factors in Qinghai-Tibet Plateau. Environmental Ecology, 2019, 1(6): 1-7]
[29] Magurran AE. Ecological Diversity and Its Measurement. Princeton, NJ, USA: Princeton University Press, 1988: 7-45
[30] 袁铭章, 刘树堂, 陈延玲, 等. 16S rDNA扩增子测序揭示长期定位秸秆还田对土壤细菌群落的影响. 华北农学报, 2016, 31(6): 157-163 [Yuan M-Z, Liu S-T, Chen Y-L, et al. Illumina amplicon sequencing of 16S rDNA tag reveals bacteria community development of long term straw returning soil. Acta Agriculturae Boreali-Sinica, 2016, 31(6): 157-163]
[31] 丛萍, 王婧, 董建新, 等. 秸秆还田对黑土亚表层微生物群落结构的影响特征及原因分析. 农业工程学报, 2020, 36(1): 109-118 [Cong P, Wang J, Dong J-X, et al. Effects and analysis of straw returning on subsoil microbial community structure in black soil. Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(1): 109-118]
[32] 张红. 植物秸秆腐解特性及微生物多样性的响应研究. 博士论文. 杨凌: 西北农林科技大学, 2017 [Zhang H. The Response of Plant Straws Decomposition Characteristics and Microbial Diversity. PhD Thesis. Yangling: Northeast A&F University, 2017]
[33] 何玉梅, 张仁陟, 张丽华, 等. 不同耕作措施对土壤真菌群落结构与生态特征的影响. 生态学报, 2007, 27(1): 113-119 [He Y-M, Zhang R-D, Zhang L-H, et al. Effects of different tillage practices on fungi community structure and ecologic characteristics in loess soils. Acta Ecological Sinica, 2007, 27(1): 113-119
[34] Zhao SC, Li KJ, Zhou W, et al. Changes in soil microbial community, enzyme activities and organic matter fractions under long-term straw return in north-central China. Agriculture, Ecosystems & Environment, 2016, 216: 82-88
[35] 商丽荣, 万里强, 李向林. 有机肥对羊草草原土壤细菌群落多样性的影响. 中国农业科学, 2020, 53(13): 2614-2624 [Shang L-R, Wan L-Q, Li X-L. Effects of organic fertilizer on soil bacterial community diversity in Leymus chinensis steppe. Scientia Agricultura Sinica, 2020, 53(13): 2614-2624]
[36] Liu JJ, Sui YY, Yu ZH, et al. High throughput sequencing analysis of bio-geographical distribution of bacterial communities in the black soils of Northeast China. Soil Biology & Biochemistry, 2014, 70: 113-122
[37] 侯建伟, 邢存芳, 卢志宏, 等. 不同秸秆生物炭对贵州黄壤细菌群落的影响. 中国农业科学, 2018, 51(23): 4485-4495 [Hou J-W, Xing C-F, Lu Z-H, et al. Effects of the different crop straw biochars on soil bacterial community of yellow soil in Guizhou. Scientia Agricultura Sinica, 2018, 51(23): 4485-4495]
[38] 孙冰洁, 张晓平, 贾淑霞. 农田土壤理化性质对土壤微生物群落的影响. 土壤与作物, 2013, 2(3): 138-144 [Sun B-J, Zhang X-P, Jia S-X. The effect of soil physical and chemical properties on soil microbial community in agro-ecosystem. Soil and Crop, 2013, 2(3): 138-144]
[39] 张瑞平, 苟小梅, 张毅, 等. 生物有机肥与化肥配施对植烟土壤养分和真菌群落特征的影响. 西北农林科技大学学报: 自然科学版, 2020, 48(8): 1-8 [Zhang R-P, Gou X-M, Zhang Y, et al. Effects of bio-organic fertilizer combined with chemical fertilizer nutrients and fungal community characteristics in tobacco-growing soil. Journal of Northwest A&F University: Natural Sciences, 2020, 48(8): 1-8]
[40] 单鹤翔. 长期秸秆与化肥配施条件下土壤微生物群落多样性研究. 硕士论文. 北京: 中国农业科学院, 2012 [Dan H-X. Study of Soil Microbial Community Diversity under Long-term Straw-returning and Fertilization. Master Thesis. Beijing: Chinese Academy of Agricultural Sciences, 2012]

生物质调理剂对川西北高寒草地沙化土壤养分和微生物群落特征的影响

Effects of biomass conditioner on soil nutrient and microbial community characteristics of alpine desertified grassland in northwest Sichuan, China

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