应用生态学报 ›› 2021, Vol. 32 ›› Issue (11): 4085-4094.doi: 10.13287/j.1001-9332.202111.031
元晓春1,2,3,崔琚琰1,3,林开淼2,周嘉聪1,3,曾泉鑫1,3,谢欢1,3,刘苑苑1,3,徐建国4,陈岳民1,3*
出版日期:
2021-11-15
发布日期:
2022-05-15
通讯作者:
*E-mail: ymchen@ fjnu.edu.cn
作者简介:
元晓春, 女, 1990年生, 硕士研究生.主要从事森林生态研究.E-mail:1075559162@qq.com
基金资助:
YUAN Xiao-chun1,2,3, CUI Ju-yan1,3, LIN Kai-miao2, ZHOU Jia-cong1,3, ZENG Quan-xin1,3, XIE Huan1,3, LIU Yuan-yuan1,3, XU Jian-guo4, CHEN Yue-min1,3*
Online:
2021-11-15
Published:
2022-05-15
Supported by:
摘要: 为探究黄山松土壤可溶性有机质(DOM)数量和质量对短期氮(N)添加的响应及其与细菌群落的关联,在福建戴云山自然保护区设置不同N添加水平(0、40和80 kg N·hm-2·a-1)试验,采用三维荧光与平行因子联用法,并结合高通量测序手段分别对土壤DOM和细菌群落进行分析。结果表明: 与对照相比,N添加整体降低了0~10和10~20 cm土层可溶性有机碳(DOC)含量和DOM腐殖化指数(HIX),其中,高氮(80 kg N·hm-2·a-1)添加下均显著降低。平行因子分析法进一步表明N添加下DOM中类腐殖质组分(C1、C2)的相对含量降低。此外,N添加减少了富营养细菌(变形菌门、酸微菌纲)的相对丰度,而增加了贫营养细菌(斯巴达杆菌纲)的相对丰度。富营养细菌的相对丰度与HIX、C1、C2呈显著正相关,与相对易分解的类富里酸组分(C3)呈显著负相关;而贫营养细菌的情况则相反。说明N添加下不同生活策略的细菌类群对DOM中难分解和易分解组分存在明显的偏好性。我们推测N沉降加剧背景下土壤微生物生活策略的转变可能有助于DOM组分的塑造。
元晓春,崔琚琰,林开淼,周嘉聪,曾泉鑫,谢欢,刘苑苑,徐建国,陈岳民. 黄山松土壤可溶性有机质对氮添加的响应及其与细菌群落的关联[J]. 应用生态学报, 2021, 32(11): 4085-4094.
YUAN Xiao-chun, CUI Ju-yan, LIN Kai-miao, ZHOU Jia-cong, ZENG Quan-xin, XIE Huan, LIU Yuan-yuan, XU Jian-guo, CHEN Yue-min. Responses of soil dissolved organic matter to nitrogen addition and its correlation with bacterial communities in Pinus taiwanensis forest[J]. Chinese Journal of Applied Ecology, 2021, 32(11): 4085-4094.
[1] | Richter A, Burrows JP, Nüss H, et al. Increase in tropospheric nitrogen dioxide over China observed from spruce. Nature, 2005, 437: 129-132 |
[2] | Tian D, Du E, Jiang L, et al. Responses of forest ecosystems to increasing N deposition in China: A critical review. Environmental Pollution, 2018, 243: 75-86 |
[3] | Wang R, Goll D, Balkanski Y, et al. Global forest carbon uptake due to nitrogen and phosphorus deposition from 1850 to 2100. Global Change Biology, 2017, 23: 4854-4872 |
[4] | Du E, Vries WD. Nitrogen-induced new net primary production and carbon sequestration in global forests. Environmental Pollution, 2018, 242: 1476-1487 |
[5] | Wu JP, Liu WF, Zhang WX, et al. Long-term nitrogen addition changes soil microbial community and litter decomposition rate in a subtropical forest. Applied Soil Ecology, 2019, 142: 43-51 |
[6] | Cheng Y, Wang J, Wang JY, et al. Nitrogen deposition differentially affects soil gross nitrogen transformations in organic and mineral horizons. Earth-Science Reviews, 2019, 242, doi: 10.1016/j.earscirev.2019.103033 |
[7] | Wang H, Liu SR, Zhang X, et al. Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China. Soil Biology and Biochemistry, 2018, 127: 22-30 |
[8] | Forsmark B, Nordin A, Maaroufi NI, et al. Low and high nitrogen deposition rates in northern coniferous forests have different impacts on aboveground litter production, soil respiration, and soil carbon stocks. Ecosystems, 2020, 23: 1423-1436 |
[9] | Kalbitz K, Kaiser K. Ecological aspects of dissolved organic matter in soils. Geoderma, 2003, 113: 177-178 |
[10] | Wang JJ, Bowden RD, Lajtha K, et al. Long-term nitrogen addition suppresses microbial degradation, enhances soil carbon storage, and alters the molecular composition of soil organic matter. Biogeochemistry, 2019, 142: 299-313 |
[11] | Sinsabaugh RL, Zak DR, Gallo M, et al. Nitrogen deposition and dissolved organic carbon production in northern temperate forests. Soil Biology and Biochemistry, 2004, 36: 1509-1515 |
[12] | Fang HJ, Cheng SL, Yu GR, et al., Experimental nitrogen deposition alters the quantity and quality of soil dissolved organic carbon in an alpine meadow on the Qinghai-Tibetan Plateau. Applied Soil Ecology, 2014, 81: 1-11 |
[13] | Chang RY, Li N, Sun XY, et al. Nitrogen addition reduces dissolved organic carbon leaching in a montane forest. Soil Biology and Biochemistry, 2018, 127: 31-38 |
[14] | Zhou ZH, Wang CK, Zheng MH, et al. Patterns and mechanisms of responses by soil microbial communities to nitrogen addition. Soil Biology and Biochemistry, 2017, 115: 433-441 |
[15] | Lu X, Gilliam FS, Yu G, et al. Long-term nitrogen addition decreases carbon leaching in nitrogen-rich forest ecosystems. Biogeosciences Discussions, 2013, 10: 1451-1481 |
[16] | Lei ZF, Sun HF, Li Q, et al. Effects of nitrogen deposition on soil dissolved organic carbon and nitrogen in Moso bamboo plantations strongly depend on management practices. Forests, 2017, 8: 452 |
[17] | 庞学勇, 包维楷, 吴宁. 森林生态系统土壤可溶性有机质(碳)影响因素研究进展. 应用与环境生物学报, 2009, 15(3): 390-398 [Pang X-Y, Bao W-K, Wu N. Research progress on influencing factors of soil soluble organic matter (carbon) in forest ecosystem. Chinese Journal of Applied and Environmental Biology, 2009, 15(3): 390-398] |
[18] | Roth VN, Lange M, Simon C, et al. Persistence of dissolved organic matter explained by molecular changes during its passage through soil. Nature Geoscience, 2019, 12: 755-761 |
[19] | Michel K, Matzner E, Dignac MF, et al. Properties of dissolved organic matter related to soil organic matter quality and nitrogen additions in Norway spruce forest floors. Geoderma, 2006, 130: 250-264 |
[20] | Yuan XC, Si YT, Lin WS, et al. Effects of short-term warming and nitrogen addition on the quantity and quality of dissolved organic matter in a subtropical Cunninghamia lanceolata plantation. PLoS One, 2018, 13(1): e0191403 |
[21] | Li X, Chen QL, He C, et al. Organic carbon amendments affect the chemodiversity of soil dissolved organic matter and its associations with soil microbial communities. Environmental Science and Technology, 2019, 53: 50-59 |
[22] | Pang HL, Chen YW, He JG, et al. Cation exchange resin-induced hydrolysis for improving biodegradability of waste activated sludge: Characterization of dissolved organic matters and microbial community. Bioresource Technology, 2020, 302: 122870 |
[23] | Ho A, Lonardo DPD, Bodelier PLE. Revisiting life strategy concepts in environmental microbial ecology. FEMS Microbiology Ecology, 2017, 93, doi: 10.1093/femsec/fix006 |
[24] | Fierer N, Bradford MA, Jackson RB. Toward an ecological classification of soil bacteria. Ecology, 2007, 88: 1354-1364 |
[25] | Shao PS, Lynch L, Xie HT, et al. Tradeoffs among microbial life history strategies influence the fate of microbial residues in subtropical forest soils. Soil Biology and Biochemistry, 2021, 153: 108112 |
[26] | Zhang KP, Ni YY, Liu XJ, et al. Microbes changed their carbon use strategy to regulate the priming effect in an 11-year nitrogen addition experiment in grassland. Science of the Total Environment, 2020, 727: 138645 |
[27] | Fierer N, Lauber CL, Ramirez KS, et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. The ISME Journal, 2012, 6: 1007-1017 |
[28] | Ramirez KS, Craine JM, Fierer N. Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes. Global Change Biology, 2012, 18: 1918-1927 |
[29] | 郑勇, 贺纪正. 森林土壤微生物对干旱和氮沉降的响应. 应用生态学报, 2020, 31(7): 2464-2472 [Zheng Y, He J-Z. Responses of forest soil microbial communities to drought and nitrogen deposition. Chinese Journal of Applied Ecology, 2020, 31(7): 2464-2472] |
[30] | Xu S, Lu WJ, Liu YT, et al. Structure and diversity of bacterial communities in two large sanitary landfills in China as revealed by high-throughput sequencing (MiSeq). Waste Management, 2017, 63: 41-48 |
[31] | 郝亚群, 谢麟, 陈岳民, 等. 中亚热带地区N沉降对杉木幼林土壤细菌群落多样性及组成的影响. 应用生态学报, 2018, 29(1): 53-58 [Hao Y-Q, Xie L, Chen Y-M, et al. Effects of nitrogen deposition on diversity and composition of soil bacterial community in a subtropical Cunninghamia lanceolata plantation. Chinese Journal of Applied Ecology, 2018, 29(1): 53-58] |
[32] | 苏松锦, 刘金福, 兰思仁, 等. 黄山松研究综述(1960-2014)及其知识图谱分析. 福建农林大学学报: 自然科学版, 2015, 44(5): 478-486 [Su S-M, Liu J-F, Lan S-R, et al. A review of Pinus taiwanensis studies (1960-2014) and the knowledge domain analysis. Journal of Fujian Agriculture and Forestry University: Natural Science, 2015, 44(5): 478-486] |
[33] | 程蕾, 林开淼, 陈岳民, 等, 氮沉降对毛竹林土壤可溶性有机质数量与光谱学特征的影响.应用生态学报, 2019, 30(5): 1754-1762 [Cheng L, Lin K-M, Chen Y-M, et al. Effects of nitrogen deposition on the concentration and spectral characteristics of dissolved organic matter in soil in Moso bamboo plantations. Chinese Journal of Applied Ecology, 2019, 30(5): 1754-1762] |
[34] | Tian D, Jiang L, Ma SH, et al. Effects of nitrogen depo-sition on soil microbial communities in temperate and subtropical forests in China. Science of the Total Environment, 2017, 607: 1367-1375 |
[35] | 曾晓敏, 范跃新, 陈岳民, 等.亚热带不同植被类型土壤磷组分特征及其影响因素. 应用生态学报, 2018, 29(7): 2156-2162 [Zeng X-M, Fan Y-X, Chen Y-M, et al. Characteristics of soil phosphorus components and their influencing factors in different subtropical vegetation types. Chinese Journal of Applied Ecology, 2018, 29(7): 2156-2162] |
[36] | Brookes PC, Powlson DS, Jenkinson DS. Measurement of microbial biomass phosphorus in soil. Soil Biology and Biochemistry, 1982, 14: 319-329 |
[37] | Bu XL, Wang LM, Ma WB, et al. Spectroscopic chara-cterization of hot-water extractable organic matter from soils under four different vegetation types along an elevation gradient in the Wuyi Mountains. Geoderma, 2010, 159: 139-146 |
[38] | 元晓春, 袁硕, 陈岳民, 等. 氮沉降对杉木人工幼林土壤溶液可溶性有机物质浓度及光谱学特征的影响. 应用生态学报, 2017, 28(1): 1-11 [Yuan X-C, Yuan S, Chen Y-M, et al. Effects of nitrogen deposition on the concentration and spectral characteristics of dissolved organic matter in soil solution in a young Cunninghamia lanceolata plantation. Chinese Journal of Applied Ecology, 2017, 28(1): 1-11] |
[39] | Munyaka PM, Eissa N, Bernstein CN, et al. Antepartum antibiotic treatment increases offspring susceptibility to experimental colitis: A role of the gut microbiota. PLoS One, 2015, 10(11): e0142536 |
[40] | Cui JY, Yuan XC, Zhang QF, et al. Nutrient availability is a dominant predictor of soil bacterial and fungal community composition after nitrogen addition in subtropical acidic forests. PLoS One, 2021, 16(2): e0246263 |
[41] | Fierer N, Bradford MA, Jackson RB. Toward an ecologi-cal classification of soil bacteria. Ecology, 2007, 88: 1354-1364 |
[42] | Pascault N, Ranjard L, Kaisermann A, et al. Stimulation of different functional groups of bacteria by various plant residues as a driver of soil priming effect. Ecosystems, 2013, 16: 810-822 |
[43] | Bergmann GT, Bates ST, Eilers KG, et al. The under-recognized dominance of Verrucomicrobia in soil bacterial communities. Soil Biology and Biochemistry, 2011, 43: 1450-1455 |
[44] | Ling N, Chen D, Guo H, et al. Differential responses of soil bacterial communities to long-term N and P inputs in a semi-arid steppe. Geoderma, 2017, 292: 25-33 |
[45] | Ma JY, Kang FF, Cheng XQ, et al. Response of soil organic carbon and nitrogen to nitrogen deposition in a Larix principis-rupprechtii plantation. Scientific Reports, 2018, 8: 8638 |
[46] | Wang C, Lu XK, Mori TK, et al. Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest. Soil Biology and Biochemistry, 2018, 121: 103-112 |
[47] | Liu XC, Zhang S. Nitrogen addition shapes soil enzyme activity patterns by changing pH rather than the composition of the plant and microbial communities in an alpine meadow soil. Plant and Soil, 2019, 440: 11-24 |
[48] | Bolan N, Adriano DC, Kunhikrishnan A, et al. Dissolved organic matter: Biogeochemistry, dynamics, and environmental significance in soils. Advances in Agronomy, 2011, 110: 1-75 |
[49] | Xiao W, Chen X, Jing X, et al. A meta-analysis of soil extracellular enzyme activities in response to global change. Soil Biology and Biochemistry, 2018, 123: 21-32 |
[50] | Mulvaney RL, Khan SA, Ellsworth TR. Synthetic nitrogen fertilizers deplete soil nitrogen: A global dilemma for sustainable cereal production. Journal of environmental quality, 2009, 38: 2295-2314 |
[51] | Wei W, Yang M, Liu YX, et al. Fertilizer N application rate impacts plant-soil feedback in a sanqi production system. Science of the Total Environment, 2018, 633: 796-807 |
[52] | Chen LY, Liu L, Mao C, et al. Nitrogen availability regulates topsoil carbon dynamics after permafrost thaw by altering microbial metabolic efficiency. Nature Communications, 2018, 9: 1-11 |
[53] | Zhang W, Zhou YQ, Jeppesen E, et al. Linking heterotrophic bacterioplankton community composition to the optical dynamics of dissolved organic matter in a large eutrophic Chinese lake. Science of the Total Environment, 2019, 679: 136-147 |
[54] | Zhang L, Liu H, Peng Y, et al. Characteristics and significance of dissolved organic matter in river sediments of extremely water-deficient basins: A Beiyun River case study. Journal of Cleaner Production, 2020, 277: 123063 |
[55] | Yang CM, Sun JL, Chen YZ, et al. Linkage between water soluble organic matter and bacterial community in sediment from a shallow, eutrophic lake, Lake Chaohu, China. Journal of Environmental Sciences, 2020, 98: 39-46 |
[56] | Thiel V, Fukushima SI, Kanno N, et al. Chloroflexi. Encyclopedia of Microbiology, 2019, doi:10.1016/B978-0-12-809633-8.20771-1 |
[57] | Liu SJ, Xi BD, Qiu ZP, et al. Succession and diversity of microbial communities in landfills with depths and ages and its association with dissolved organic matter and heavy metals. Science of the Total Environment, 2019, 651: 909-916 |
[58] | Traving SJ, Rowe O, Jakobsen NM, et al. The effect of increased loads of dissolved organic matter on estuarine microbial community composition and function. Frontiers in Microbiology, 2017, 8: 351 doi:10.3389/fmicb.2017.00351 |
[59] | Nie YX, Wang MG, Zhang W, et al. Ammonium nitrogen content is a dominant predictor of bacterial community composition in an acidic forest soil with exogenous nitrogen enrichment. Science of the Total Environment, 2018, 624: 407-415 |
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