杉木林土壤丛枝菌根真菌形态特征及孢子相关细菌多样性对模拟氮沉降和干旱的响应

doi:10.13287/j.1001-9332.202312.017

应用生态学报 ›› 2023, Vol. 34 ›› Issue (12): 3291-3300.doi: 10.13287/j.1001-9332.202312.017

杉木林土壤丛枝菌根真菌形态特征及孢子相关细菌多样性对模拟氮沉降和干旱的响应

史加勉^1,2, 宋鸽^1,2, 刘珊珊^1,2, 郑勇^1,2,3*

¹福建师范大学湿润亚热带生态地理过程教育部重点实验室, 福州 350117;
²福建师范大学地理科学学院, 福州 350117;
³福建三明森林生态系统国家野外科学观测研究站, 福建三明 365002

收稿日期:2023-09-11 修回日期:2023-10-29 出版日期:2023-12-15 发布日期:2024-06-15
通讯作者: *E-mail: zhengy@fjnu.edu.cn
作者简介:史加勉, 女, 1998年生, 硕士研究生。主要从事真菌生态学研究。E-mail: sjm1692413509@163.com
基金资助:
国家自然科学基金项目(31971447)和福建省自然科学基金项目(2022J02025)

Responses of arbuscular mycorrhizal fungal morphological traits and the diversity of spore-associated bacteria to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation soil

SHI Jia-mian^1,2, SONG Ge^1,2, LIU Shanshan^1,2, ZHENG Yong^1,2,3*

¹Key Laboratory for Humid Subtropical Eco-geographi-cal Processes of the Ministry of Education, Fujian Normal University, Fuzhou 350117, China;
²School of Geographi-cal Sciences, Fujian Normal University, Fuzhou 350117, China;
³Sanming Forest Ecosystem National Observation and Research Station, Sanming 365002, Fujian, China

Received:2023-09-11 Revised:2023-10-29 Online:2023-12-15 Published:2024-06-15

摘要/Abstract

摘要： 丛枝菌根(AM)真菌对促进宿主植物的养分吸收和提高抗逆能力具有重要作用。土壤中AM真菌孢子相关细菌能够促进菌根定殖,但其对环境变化的响应知之甚少。本研究以杉木人工林表层土壤为对象,在分析AM真菌形态学指标的基础上,重点探究孢子相关细菌群落结构对模拟氮沉降(40 kg N·hm^-2·a^-1)和干旱(-50%穿透雨)的响应及季节差异。结果表明: 氮添加、隔离降雨分别显著影响AM真菌的孢子密度与根外菌丝长度;季节显著影响AM真菌根系侵染率、根外菌丝长度及孢子密度。与对照(不进行氮添加和不隔离降雨处理)相比,氮添加、隔离降雨在冬季均显著降低了孢子密度;单独隔离降雨、氮添加和隔离降雨同时处理在夏季均显著增加了根外菌丝长度。AM真菌孢子中相对多度占优势的细菌包括变形菌门、酸杆菌门、放线菌门、绿弯菌门、浮霉菌门等。氮添加和隔离降雨单独处理对孢子相关细菌多样性无显著影响;氮添加和隔离降雨同时处理显著改变了细菌群落组成,两个季节间差异显著,且夏季细菌的丰富度显著高于冬季,群落变异度小于冬季。土壤总氮、硝态氮和可溶性有机碳含量显著影响孢子相关细菌群落组成。综上,氮添加、隔离降雨对AM真菌形态特征的影响具有季节依赖性,氮添加和隔离降雨同时处理对孢子相关细菌群落结构有明显影响。

关键词: 模拟氮沉降, 干旱, 丛枝菌根真菌, 孢子相关细菌, 多样性, 群落结构

Abstract: Arbuscular mycorrhizal (AM) fungi play an important role in plant nutrient absorption and stress resistance. AM fungal spore-associated bacteria are essential for mycorrhizal colonization, but their responses to environmental changes remain largely unknown. We collected surface soil samples from a Chinese fir plantation in both summer and winter to investigate the responses of AM fungal morphological traits and spore-associated bacterial communities to simulated nitrogen deposition (40 kg N·hm^-2·a^-1 addition) and drought (-50% precipitation exclusion). Our results showed that nitrogen addition and precipitation exclusion significantly affected AM fungal spore density and extraradical hyphal length, respectively. AM fungal intraradical colonization rate, extraradical hyphal length and spore density were significantly differed between the two seasons. Compared to control (no nitrogen addition and no precipitation exclusion treatment), both nitrogen addition and precipitation exclusion significantly reduced spore density in winter, while precipitation exclusion alone and the combined nitrogen addition and precipitation exclusion significantly increased extraradical hyphal length in summer. The dominant spore-associated bacterial phyla were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, and Planctomycetes. Nitrogen addition and precipitation exclusion did not affect the diversity of spore-associated bacteria. However, the combined nitrogen addition and precipitation exclusion treatment altered the composition of the bacterial community, with significant variations between the two seasons. The spore-associated bacterial diversity was significantly higher and community variability (or turnover) was lower during summer than winter. Soil total nitrogen, nitrate nitrogen and dissolved organic carbon were important factors influencing the bacterial community composition. In all, the effects of nitrogen addition and precipitation exclusion on the morphological traits of AM fungi are seasonally dependent. The combination of nitrogen addition and precipitation exclusion has a significant impact on AM fungal spore-associated bacterial community structure.

Key words: simulated nitrogen deposition, drought, arbuscular mycorrhizal fungi, spore-associated bacteria, diversity, community structure

史加勉, 宋鸽, 刘珊珊, 郑勇. 杉木林土壤丛枝菌根真菌形态特征及孢子相关细菌多样性对模拟氮沉降和干旱的响应[J]. 应用生态学报, 2023, 34(12): 3291-3300.

SHI Jia-mian, SONG Ge, LIU Shanshan, ZHENG Yong. Responses of arbuscular mycorrhizal fungal morphological traits and the diversity of spore-associated bacteria to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation soil[J]. Chinese Journal of Applied Ecology, 2023, 34(12): 3291-3300.

参考文献

[1] Gianinazzi-Pearson V. Plant cell responses to arbuscular mycorrhizal fungi: Getting to the roots of the symbiosis. The Plant Cell, 1996, 8: 1871-1883
[2] Bennett AE, Groten K. The costs and benefits of plant-arbuscular mycorrhizal fungal interactions. Annual Review of Plant Biology, 2022, 73: 649-672
[3] Giovannetti M, Avio L, Sbrana C. Fungal spore germination and pre-symbiotic mycelial growth: Physiological and genetic aspects// Koltai H, Kapulnik Y, ed. Arbuscular Mycorrhizas: Physiology and Function. Dordrecht: Springer, 2010: 3-32
[4] Ujvári G, Turrini A, Avio L, et al. Possible role of arbuscular mycorrhizal fungi and associated bacteria in the recruitment of endophytic bacterial communities by plant roots. Mycorrhiza, 2021, 31: 527-544
[5] Sangwan S, Prasanna R. Mycorrhizae helper bacteria: Unlocking their potential as bioenhancers of plant-arbuscular mycorrhizal fungal associations. Microbial Ecology, 2022, 84: 1-10
[6] Selvakumar G, Krishnamoorthy R, Kim K, et al. Genetic diversity and association characters of bacteria isolated from arbuscular mycorrhizal fungal spore walls. PLoS One, 2016, 11: e0160356
[7] Ackerman D, Millet DB, Chen X. Global estimates of inorganic nitrogen deposition across four decades. Global Biogeochemical Cycles, 2019, 33: 100-107
[8] Liu XC, Zhang ST. Nitrogen addition shapes soil enzyme activity patterns by changing pH rather than the composition of the plant and microbial communities in an alpine meadow soil. Plant and Soil, 2019, 440: 11-24
[9] Pan S, Wang Y, Qiu Y, et al. Nitrogen-induced acidification, not N-nutrient, dominates suppressive N effects on arbuscular mycorrhizal fungi. Global Change Biology, 2020, 26: 6568-6580
[10] Yadav DS, Jaiswal B, Gautam M, et al. Soil acidification and its impact on plants// Singh P, Singh SK, Prasad SM, eds. Plant Responses to Soil Pollution. Singapore: Springer, 2020: 1-26
[11] 史加勉, 王聪, 郑勇, 等. 丛枝菌根真菌形态结构、物种多样性和群落组成对氮沉降响应研究进展. 菌物学报, 2023, 42(1): 118-129
[12] Lilleskov EA, Kuyper TW, Bidartondo MI, et al. Atmospheric nitrogen deposition impacts on the structure and function of forest mycorrhizal communities: A review. Environmental Pollution, 2019, 246: 148-162
[13] Peng SL, Ban MJ, Xing W, et al. Effects of nitrogen addition and seasonal change on arbuscular mycorrhizal fungi community diversity in a poplar plantation. Frontiers in Ecology and Evolution, 2022, 10: 1101698
[14] Han YF, Feng JG, Han MG, et al. Responses of arbuscular mycorrhizal fungi to nitrogen addition: A meta analysis. Global Change Biology, 2020, 26: 7229-7241
[15] Ma XC, Geng QH, Zhang HG, et al. Global negative effects of nutrient enrichment on arbuscular mycorrhizal fungi, plant diversity and ecosystem multifunctionality. New Phytologist, 2021, 229: 2957-2969
[16] Liu MH, Shen YK, Li Q, et al. Arbuscular mycorrhizal fungal colonization and soil pH induced by nitrogen and phosphorus additions affects leaf C:N:P stoichiometry in Chinese fir (Cunninghamia lanceolata) forests. Plant and Soil, 2021, 461: 421-440
[17] Liu YJ, Shi GX, Mao L, et al. Direct and indirect influences of 8 yr of nitrogen and phosphorus fertilization on Glomeromycota in an alpine meadow ecosystem. New Phytologist, 2012, 194: 523-535
[18] Johnson NC, Rowland DL, Corkidi L, et al. Nitrogen enrichment alters mycorrhizal allocation at five mesic to semiarid grasslands. Ecology, 2003, 84: 1895-1908
[19] Zhang T, Yang X, Guo R, et al. Response of AM fungi spore population to elevated temperature and nitrogen addition and their influence on the plant community composition and productivity. Scientific Reports, 2016, 6: 24749
[20] Babalola BJ, Li J, Willing CE, et al. Nitrogen fertilisation disrupts the temporal dynamics of arbuscular mycorrhizal fungal hyphae but not spore density and community composition in a wheat field. New Phytologist, 2022, 234: 2057-2072
[21] Jansson JK, Hofmockel KS. Soil microbiomes and climate change. Nature Reviews Microbiology, 2020, 18: 35-46
[22] Lesk C, Rowhani P, Ramankutty N. Influence of extreme weather disasters on global crop production. Nature, 2016, 529: 84-87
[23] Dong J, Jiang YM, Lyu MK, et al. Drought changes the trade-off strategy of root and arbuscular mycorrhizal fungi growth in a subtropical Chinese fir plantation. Forests, 2023, 14: 114
[24] Gupta A, Rico-Medina A, Caño-Delgado AI. The physiology of plant responses to drought. Science, 2020, 368: 266-269
[25] Ploughe LW, Jacobs EM, Frank GS, et al. Community Response to Extreme Drought (CRED): A framework for drought-induced shifts in plant-plant interactions. New Phytologist, 2019, 222: 52-69
[26] Sardans J, Urbina I, Grau O, et al. Long-term drought decreases ecosystem C and nutrient storage in a Mediterranean holm oak forest. Environmental and Experimental Botany, 2020, 177: 104135
[27] Bahadur A, Batool A, Nasir F, et al. Mechanistic insights into arbuscular mycorrhizal fungi-mediated drought stress tolerance in plants. International Journal of Mole-cular Sciences, 2019, 20: 4199
[28] Sepahvand T, Etemad V, Matinizade M, et al. Symbiosis of AMF with growth modulation and antioxidant capacity of Caucasian Hackberry (Celtis caucasica L.) seedlings under drought stress. Central Asian Journal of Environmental Science and Technology Innovation, 2021, 2: 20-35
[29] Zhang F, Zou YN, Wu QS. Quantitative estimation of water uptake by mycorrhizal extraradical hyphae in citrus under drought stress. Scientia Horticulturae, 2018, 229: 132-136
[30] Wu QS, Zou YN. Arbuscular mycorrhizal fungi and tolerance of drought stress in plants// Wu QS, ed. Arbuscular Mycorrhizas and Stress Tolerance of Plants. Singapore: Springer, 2017: 25-41
[31] Gopal S, Chandrasekaran M, Shagol C, et al. Spore associated bacteria (SAB) of arbuscular mycorrhizal fungi (AMF) and plant growth promoting rhizobacteria (PGPR) increase nutrient uptake and plant growth under stress conditions. Korean Journal of Soil Science and Fertilizer, 2012, 45: 582-592
[32] 余朝晖. 不同林龄杉木人工林生态功能比较研究. 林业勘察设计, 2018, 38(3): 6-14
[33] Cao JL, Lin TC, Yang ZJ, et al. Warming exerts a stronger effect than nitrogen addition on the soil arbuscular mycorrhizal fungal community in a young subtropical Cunninghamia lanceolata plantation. Geoderma, 2020, 367: 114273
[34] 宋鸽, 李晓杰, 王全成, 等. 杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应. 应用生态学报, 2022, 33(9): 2388-2396
[35] 李冬萍, 廖楠, 汪茜, 等. 木薯根系丛枝菌根真菌染色方法. 亚热带农业研究, 2016, 12(1): 50-55
[36] Miller RM, Jastrow JD, Reinhardt DR. External hyphal production of vesicular-arbuscular mycorrhizal fungi in pasture and tallgrass prairie communities. Oecologia, 1995, 103: 17-23
[37] Daniels BA, Skipper HD. Methods for the recovery and quantitative estimation of propagules from soil// Schenck NC, ed. Methods and Principles of Mycorrhizal Research. Saint Paul: American Phytopathological Society, 1982: 29-37
[38] Lin CY, Wang YX, Liu MH, et al. Effects of nitrogen deposition and phosphorus addition on arbuscular mycorrhizal fungi of Chinese fir (Cunninghamia lanceolata). Scientific Reports, 2020, 10: 12260
[39] Johnson NC. Resource stoichiometry elucidates the structure and function of arbuscular mycorrhizas across scales. New Phytologist, 2010, 185: 631-647
[40] Lü PP, Zheng Y, Chen L, et al. Irrigation and fertilization effects on arbuscular mycorrhizal fungi depend on growing season in a dryland maize agroecosystem. Pedobiologia, 2020, 83: 150687
[41] Becerra AG, Cabello M, Zak MR, et al. Arbuscular mycorrhizae of dominant plant species in Yungas forests, Argentina. Mycologia, 2009, 101: 612-621
[42] Maitra P, Zheng Y, Chen L, et al. Effect of drought and season on arbuscular mycorrhizal fungi in a subtropical secondary forest. Fungal Ecology, 2019, 41: 107-115
[43] Zhao CT, Lin QH, Tian D, et al. Nitrogen addition promotes conservative resource-use strategies via aggravating phosphorus limitation of evergreen trees in subtropical forest. Science of the Total Environment, 2023, 889: 164047
[44] Jiang FY, Zhang L, Zhou JC, et al. Arbuscular mycorrhizal fungi enhance mineralisation of organic phosphorus by carrying bacteria along their extraradical hyphae. New Phytologist, 2021, 230: 304-315
[45] Gemma JN, Koske RE. Seasonal variation in spore abundance and dormancy of Gigaspora gigantea and in mycorrhizal inoculum potential of a dune soil. Mycologia, 1988, 80: 211-216
[46] 赵爱花, 刘蕾, 付伟, 等. 施氮对森林生态系统AM真菌群落组成及多样性的影响. 生态学报, 2020, 40(21): 7576-7587
[47] Iffis B, St-Arnaud M, Hijri M. Petroleum hydrocarbon contamination, plant identity and arbuscular mycorrhizal fungal (AMF) community determine assemblages of the AMF spore-associated microbes. Environmental Microbiology, 2016, 18: 2689-2704
[48] Battini F, Cristani C, Giovannetti M, et al. Multifunctionality and diversity of culturable bacterial communities strictly associated with spores of the plant beneficial symbiont Rhizophagus intraradices. Microbiological Research, 2016, 183: 68-79
[49] Rozmoš M, Bukovská P, Hršelová H, et al. Organic nitrogen utilisation by an arbuscular mycorrhizal fungus is mediated by specific soil bacteria and a protist. ISME Journal, 2022, 16: 676-685

杉木林土壤丛枝菌根真菌形态特征及孢子相关细菌多样性对模拟氮沉降和干旱的响应

Responses of arbuscular mycorrhizal fungal morphological traits and the diversity of spore-associated bacteria to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation soil

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张煜林, 刘玲娟, 刘胜龙, 方万力, 骆珍莎, 洪宣生, 成向荣. 不同密度杉木萌生林自然恢复初期群落结构对生态系统碳密度的影响 [J]. 应用生态学报, 2024, 35(2): 289-297.
[2]	梁雪丽, 梁晓霞, 毛晓雅, 柴宝峰, 贾彤. 芦芽山鬼箭锦鸡儿灌丛不同深度土壤细菌群落分布格局及其影响因素 [J]. 应用生态学报, 2024, 35(2): 381-389.
[3]	窦寒梅, 赵锐锋, 陈喜东, 石晶, 王景发, 刘福寿. 西北干旱区国土空间生态修复优先区识别——以黑河流域张掖市为例 [J]. 应用生态学报, 2024, 35(2): 469-479.
[4]	吴德东, 刘志民, 曹宇. 半干旱风沙区“山水林田湖草沙”建设中防护林营建的原理与方法 [J]. 应用生态学报, 2024, 35(1): 17-24.
[5]	陈晴, 王佳啸, 王一凡, 王杨, 王艳. 辽西北荒漠化区植物群落分异 [J]. 应用生态学报, 2024, 35(1): 41-48.
[6]	张悦, 马巍格, 刘谷娥, 周全来, 郭佳, 曹伟. 东北半干旱区外来植物入侵现状评估 [J]. 应用生态学报, 2024, 35(1): 73-79.
[7]	李菁, 张小飞, 张惠雯, 文嘉庆, 张焱, 徐玲玲. 盐胁迫对白及根际细菌群落组成及多样性的影响 [J]. 应用生态学报, 2024, 35(1): 219-228.
[8]	尹必然, 向涌旗, 吕倩, 张妍, 陈雨芩, 陈刚, 赖家明, 李贤伟. 目标树经营对马尾松人工林林下更新的影响 [J]. 应用生态学报, 2023, 34(8): 2047-2054.
[9]	黄睿智, 王奇, 孙婧依, 杨绍微, 赵倚霈, 刘建锋, 肖文发. 太白山南北坡栎类林物种组成与群落特征比较 [J]. 应用生态学报, 2023, 34(8): 2055-2064.
[10]	解萍萍, 张博奕, 董一博, 吕鹏程, 杜明超, 张先亮. 华北落叶松和白杄径向生长对干旱的生态弹性差异 [J]. 应用生态学报, 2023, 34(7): 1779-1786.
[11]	卢子欣, 杨漫, 李彬, 胡俊杰, 于海彬. 喜马拉雅山脉种子植物海拔梯度分布格局及其影响因素 [J]. 应用生态学报, 2023, 34(7): 1787-1796.
[12]	黄庆阳, 谢立红, 曹宏杰, 王立民, 杨帆, 王继丰, 刘赢男, 倪红伟. 细菌对五大连池火山森林凋落物早期分解的影响 [J]. 应用生态学报, 2023, 34(7): 1941-1948.
[13]	熊涵, 刘彦伶, 李渝, 张雅蓉, 黄兴成, 杨叶华, 朱华清, 蒋太明. 长期不同施肥模式对黄壤旱地土壤细菌群落结构和土壤养分的影响 [J]. 应用生态学报, 2023, 34(7): 1949-1956.
[14]	俞锦丽, 陈旭, 张颖, 朱颖婷, 张文慧, 罗思琦, 刘淑丽. 鄱阳湖湿地水体、沉积物及微塑料表面细菌群落结构特征 [J]. 应用生态学报, 2023, 34(7): 1968-1974.
[15]	郭蓉, 吴旭东, 王占军, 蒋齐, 俞鸿千, 贺婧, 刘文娟, 马琨. 荒漠草原土壤细菌和真菌群落对降水变化的响应 [J]. 应用生态学报, 2023, 34(6): 1500-1508.